Cat. No.: HY-N1166

Tephrosin

(Synonyms: Deguelinol I; Hydroxydeguelin)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Tephrosin is a natural rotenoid which has potent antitumor activities. Tephrosin induces degradation of of EGFR and ErbB2 by inducing internalization of the receptors.

For research use only. We do not sell to patients.

Purity: 98.02%
CAS No.: 76-80-2
Formula: C₂₃H₂₂O₇
Molecular Weight:410.42

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Tephrosin Chemical Structure

CAS No.: 76-80-2

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Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Tephrosin is a natural rotenoid which has potent antitumor activities. Tephrosin induces degradation of of EGFR and ErbB2 by inducing internalization of the receptors^[1].

All EGFR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
697	IC₅₀	5.4 μM Compound: 9	Cytotoxicity against human 697 cells after 48 hrs by MTS assay Cytotoxicity against human 697 cells after 48 hrs by MTS assay	[PMID: 23895019]
697	IC₅₀	9 μM Compound: 9	Cytotoxicity against human 697 cells after 72 hrs by MTS assay Cytotoxicity against human 697 cells after 72 hrs by MTS assay	[PMID: 23895019]
A2780	IC₅₀	9.1 μg/mL Compound: 8	Cytotoxicity against human A2780 cells Cytotoxicity against human A2780 cells	[PMID: 15043430]
A549	ED₅₀	<0.001 μg/mL Compound: 9	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 8326318]
B16	IC₅₀	1.35 μM Compound: 9	Cytotoxicity against mouse B16 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse B16 cells assessed as reduction in cell viability by MTT assay	[PMID: 28665590]
HCT-116	IC₅₀	0.04 ug Compound: 7	Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting	[PMID: 21452840]
HCT-116	IC₅₀	0.04 μg Compound: 7	Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting	[PMID: 21452840]
HCT-8	ED₅₀	0.09 μg/mL Compound: 9	Cytotoxicity against human HCT8 cells Cytotoxicity against human HCT8 cells	[PMID: 8326318]
HeLa	IC₅₀	0.11 μM Compound: 1	Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells by SEAP reporter gene assay Inhibition of TNF-alpha-induced NF-kappaB activation in human HeLa cells by SEAP reporter gene assay	[PMID: 18841906]
HEp-2	CC₅₀	12.5 μM Compound: 9	Cytotoxicity against human HEp-2 cells measured after 3 days by CellTiter 96 aqueous one solution assay (Rvb >100 microM) Cytotoxicity against human HEp-2 cells measured after 3 days by CellTiter 96 aqueous one solution assay (Rvb >100 microM)	[PMID: 38579352]
HEp-2	IC₅₀	0.8 μM Compound: 9	Antiviral activity against Human respiratory syncytial virus infected in human HEp-2 cells assessed as plaque reduction preincubated with cells 15 mins followed by viral infection for 2.5 hrs and measured after 3 days by crystal violet staining based micr Antiviral activity against Human respiratory syncytial virus infected in human HEp-2 cells assessed as plaque reduction preincubated with cells 15 mins followed by viral infection for 2.5 hrs and measured after 3 days by crystal violet staining based micr	[PMID: 38579352]
HepG2	IC₅₀	1.35 μM Compound: 9	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTT assay	[PMID: 28665590]
HT-29	IC₅₀	>10 μM Compound: 9	Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay	[PMID: 23895019]
KB	ED₅₀	0.36 μg/mL Compound: 9	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 8326318]
Lewis lung carcinoma cell line	IC₅₀	1.35 μM Compound: 9	Cytotoxicity against mouse LL/2 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse LL/2 cells assessed as reduction in cell viability by MTT assay	[PMID: 28665590]
LNCaP	ED₅₀	0.3 μM Compound: 8	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 32459967]
P388	ED₅₀	0.06 μg/mL Compound: 9	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 8326318]
Raji	IC₅₀	>10 μM Compound: 9	Cytotoxicity against human Raji cells after 48 hrs by MTS assay Cytotoxicity against human Raji cells after 48 hrs by MTS assay	[PMID: 23895019]
Raji	IC₅₀	>10 μM Compound: 9	Cytotoxicity against human Raji cells after 72 hrs by MTS assay Cytotoxicity against human Raji cells after 72 hrs by MTS assay	[PMID: 23895019]
RAW264.7	IC₅₀	2 μM Compound: 1	Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells by SEAP reporter gene assay Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells by SEAP reporter gene assay	[PMID: 18841906]
RPMI-7951	ED₅₀	0.07 μg/mL Compound: 9	Cytotoxicity against human RPMI7951 cells Cytotoxicity against human RPMI7951 cells	[PMID: 8326318]
TE-671	ED₅₀	0.05 μg/mL Compound: 9	Cytotoxicity against human TE671 cells Cytotoxicity against human TE671 cells	[PMID: 8326318]
U-937	IC₅₀	23 μM Compound: 2, tephrosin	Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	30 μM Compound: 2, tephrosin	Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	7.3 μM Compound: 2, tephrosin	Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay	[PMID: 17158054]

In Vitro

Treatment of HT-29 cells with tephrosin (0-10μM) inhibits both the ligand-induced and constitutive phosphorylation of EGFR, ErbB2 and ErbB3, and concomitantly suppresses the activation of the downstream signaling molecules such as Akt and Erk1/2 mitogen-activated protein kinase (MAPK) ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 76-80-2
Appearance Solid
Molecular Weight 410.42
Formula C₂₃H₂₂O₇
Color White to off-white
SMILES

O=C1[C@]2(O)[C@](COC3=CC(OC)=C(OC)C=C32)([H])OC4=C5C=CC(C)(C)OC5=CC=C14
Synonyms

Deguelinol I; Hydroxydeguelin
Structure Classification
- Flavonoids
- Isoflavanones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

Biomedicines

Tephrosin Suppresses the Chemoresistance of Paclitaxel-Resistant Ovarian Cancer via Inhibition of FGFR1 Signaling Pathway. [Abstract]

2023 Nov 27;11(12):3155. PMID: 38137377
J Cancer

Tephrosin induces apoptosis of human pancreatic cancer cells through the generation of reactive oxygen species. [Abstract]

2021 Jan 1;12(1):270-280. PMID: 33391424
Turk J Biochem

2025 Oct 9.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (243.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4365 mL

12.1826 mL

24.3653 mL

5 mM

0.4873 mL

2.4365 mL

4.8731 mL

10 mM

0.2437 mL

1.2183 mL

2.4365 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (459 KB)
- English - EN (459 KB)
- Français - FR (459 KB)
- Deutsch - DE (459 KB)
- Norwegian - NO (459 KB)
- Español - ES (459 KB)
- Swedish - SV (459 KB)
- Italian - IT (459 KB)
- Portuguese - PT (459 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Choi S, et al. Tephrosin induces internalization and degradation of EGFR and ErbB2 in HT-29 human colon cancer cells. Cancer Lett. 2010 Jul 1;293(1):23-30. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4365 mL	12.1826 mL	24.3653 mL	60.9132 mL
	5 mM	0.4873 mL	2.4365 mL	4.8731 mL	12.1826 mL
	10 mM	0.2437 mL	1.2183 mL	2.4365 mL	6.0913 mL
	15 mM	0.1624 mL	0.8122 mL	1.6244 mL	4.0609 mL
	20 mM	0.1218 mL	0.6091 mL	1.2183 mL	3.0457 mL
	25 mM	0.0975 mL	0.4873 mL	0.9746 mL	2.4365 mL
	30 mM	0.0812 mL	0.4061 mL	0.8122 mL	2.0304 mL
	40 mM	0.0609 mL	0.3046 mL	0.6091 mL	1.5228 mL
	50 mM	0.0487 mL	0.2437 mL	0.4873 mL	1.2183 mL
	60 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL
	80 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7614 mL
	100 mM	0.0244 mL	0.1218 mL	0.2437 mL	0.6091 mL

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Tephrosin

MCE Bioactive Compound Library

Natural Product Library Plus

Publications Citing Use of MedChemExpress (MCE) Tephrosin

All EGFR Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tephrosin

MCE Bioactive Compound Library

Natural Product Library Plus

Publications Citing Use of MedChemExpress (MCE) Tephrosin

All EGFR Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tephrosin Related Classifications

Cat. No.: HY-N1166