Cat. No.: HY-172458

Z-3578

1 Cited Publications

Customer Review

Based on 1 publication(s) in Google Scholar

Z-3578 is an orally active small-molecule antagonist of MrgX2 with potent antipseudoallergic activity, exhibiting a K_D value of 729 nM. Z-3578 effectively inhibits mast cell degranulation induced by substance P (SP) and C48/80, suppresses the release of β-hexosaminidase, significantly reduces the release of histamine and TNF-α, and decreases intracellular calcium flux. In a mouse pseudoallergy model, Z-3578 significantly alleviates paw swelling and dye extravasation, and reduces serum histamine levels. Z-3578 can be used for the study of pseudoallergic reactions.

For research use only. We do not sell to patients.

Purity: 98.72%
CAS No.: 473687-20-6
Formula: C₂₃H₁₆Cl₂N₄OS
Molecular Weight:467.37

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Z-3578 Chemical Structure

CAS No.: 473687-20-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All TNF Receptor Isoforms :

In Vitro

Z-3578 potently inhibits β-hexosaminidase release from human mast cell line LAD2 induced by SP (HY-P0201) and C48/80 (HY-130592), with IC₅₀ values of 4.90 μM and 6.18 μM^[1].
Z-3578 (1-10 μM) concentration-dependently inhibits SP-induced histamine release from LAD2 human mast cells^[1].
Z-3578 (1-10 μM; 6 h) concentration-dependently inhibits SP-induced TNF-α release from human mast cell line LAD2^[1].
Z-3578 (10 μM; 30 min) inhibits intracellular calcium mobilization in MrgX2-His-tag-HEK293 cells and blocks MrgX2-mediated signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

LAD2 human mast cell
Concentration:

1, 5 and 10 μM
Incubation Time:

6 h
Result:

Reduced SP-induced TNF-α release in a concentration-dependent manner.
Showed significant inhibition at all tested concentrations.

In Vivo

Z-3578 (0.1-1 mg/mL, 1-10 mg/kg; i.g.; single dose) dose-dependently inhibits C48/80-induced pseudoallergic reactions in male C57BL/6 mice, with significant reductions in paw thickness, Evans blue extravasation, serum histamine release, and mast cell degranulation observed at doses of 0.5 mg/mL, 1 mg/mL, 5 mg/kg, and 10 mg/kg^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 (male, 6-8 weeks old)^[1]
Dosage:

0.1 mg/mL; 0.5 mg/mL; 1 mg/mL; 1 mg/kg; 5 mg/kg; 10 mg/kg
Administration:

i.g.; single dose
Result:

Significantly reduced paw edema induced by C48/80 in mice at concentrations of 0.5 and 1.0 mg/mL.
Significantly decreased Evans blue extravasation at a concentration of 1.0 mg/mL.
Markedly lowered serum histamine release induced by C48/80.
Attenuated mast cell degranulation in the mouse paw skin induced by C48/80.

Chemical Information

CAS No. 473687-20-6
Appearance Solid
Molecular Weight 467.37
Formula C₂₃H₁₆Cl₂N₄OS
Color White to light yellow
SMILES

O=C(CSC1=NC(C2=CC=CC=C2)=C(N=N1)C3=CC=CC=C3)NC4=C(C=C(C=C4)Cl)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (1)

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Journal Impact Factor

Cells

Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]

2026 Feb 3;15(3):287. PMID: 41677650

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (53.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1396 mL

10.6982 mL

21.3963 mL

5 mM

0.4279 mL

2.1396 mL

4.2793 mL

10 mM

0.2140 mL

1.0698 mL

2.1396 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xia Z, et al. MrgX2-Targeting Ligand Screen for Antipseudoallergic Agents by Immobilized His-Tag-Fused Protein Technology. J Med Chem. 2025;68(5):5942-5953. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1396 mL	10.6982 mL	21.3963 mL	53.4908 mL
	5 mM	0.4279 mL	2.1396 mL	4.2793 mL	10.6982 mL
	10 mM	0.2140 mL	1.0698 mL	2.1396 mL	5.3491 mL
	15 mM	0.1426 mL	0.7132 mL	1.4264 mL	3.5661 mL
	20 mM	0.1070 mL	0.5349 mL	1.0698 mL	2.6745 mL
	25 mM	0.0856 mL	0.4279 mL	0.8559 mL	2.1396 mL
	30 mM	0.0713 mL	0.3566 mL	0.7132 mL	1.7830 mL
	40 mM	0.0535 mL	0.2675 mL	0.5349 mL	1.3373 mL
	50 mM	0.0428 mL	0.2140 mL	0.4279 mL	1.0698 mL

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Z-3578

Apoptosis Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Differentiation Inducing Compound Library

Orally Active Compound Library

Angiogenesis-Related Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Cytokine Inhibitors Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Z-3578

All TNF Receptor Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Z-3578

Apoptosis Compound Library

GPCR/G Protein Compound Library

Immunology/Inflammation Compound Library

Differentiation Inducing Compound Library

Orally Active Compound Library

Angiogenesis-Related Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Cytokine Inhibitors Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Anti-Rheumatic Arthritis Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Z-3578

All TNF Receptor Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Z-3578 Related Classifications

HY-172458 Related Classifications