2. Neuronal Signaling Metabolic Enzyme/Protease
  3. Monoamine Oxidase Endogenous Metabolite
  4. β-Aminopropionitrile hydrochloride

β-Aminopropionitrile hydrochloride  (Synonyms: BAPN hydrochloride)

Cat. No.: HY-Y1750A Purity: 99.76%
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β-Aminopropionitrile (BAPN) hydrochloride is a specific, irreversible and orally active lysyl oxidase (LOX) inhibitor. β-Aminopropionitrile hydrochloride targets the active site of LOX or LOXL isoenzymes.

For research use only. We do not sell to patients.

CAS No. : 646-03-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
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100 mg In-stock
250 mg In-stock
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Based on 13 publication(s) in Google Scholar

Other Forms of β-Aminopropionitrile hydrochloride:

β-Aminopropionitrile hydrochloride Related Antibodies

Top Publications Citing Use of Products

    β-Aminopropionitrile hydrochloride purchased from MedChemExpress. Usage Cited in: Exp Hematol Oncol. 2025 Aug 27;14(1):111.  [Abstract]

    The BAPN (50 mg/kg; once every two days for four weeks; ip) treatment process for orthotopic xenograft mice model.

    β-Aminopropionitrile hydrochloride purchased from MedChemExpress. Usage Cited in: Exp Hematol Oncol. 2025 Aug 27;14(1):111.  [Abstract]

    Western blot of vimentin, E-cadherin, CDKN1A in PBS-treated xenografts and BAPN (50 mg/kg; once every two days for four weeks; ip) -treated xenografts, using GAPDH as a control.

    β-Aminopropionitrile hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 7:76:103346.  [Abstract]

    Morphology of cerebral edema in sham, HIBD and BAPN intervention groups (100 mg/kg/ip/day) at 72 h post-surgery.

    β-Aminopropionitrile hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 7:76:103346.  [Abstract]

    Cell viability of BAPN (500 nM)-treated OGD/R-induced HT22 cells at 0 h, 12 h and 24 h after reoxygenation.

    β-Aminopropionitrile hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Sep 7:76:103346.  [Abstract]

    Representative TEM images of mitochondrial morphology in BAPN (500 nM) -incubated HT22 cells under OGD/R-induced conditions. Green arrows indicate normally shaped mitochondria, black arrows indicate damaged mitochondria.

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    • Biological Activity

    • Purity & Documentation

    • References

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    Description

    β-Aminopropionitrile (BAPN) hydrochloride is a specific, irreversible and orally active lysyl oxidase (LOX) inhibitor. β-Aminopropionitrile hydrochloride targets the active site of LOX or LOXL isoenzymes[1][2].

    IC50 & Target

    Lysyl Oxidase

     

    In Vitro

    β-Aminopropionitrile (BAPN) normalizes the expression of GLUT4 and adiponectin, and improves glucose uptake in an in vitro model of insulin resistance[1].
    β-Aminopropionitrile (500 μM; 72 h) blocks the hypoxia-induced EMT morphological and marker protein changes, and inhibits invasion and migration capacities of cervical carcinoma cells in vitro[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    β-Aminopropionitrile hydrochloride Related Antibodies

    Western Blot Analysis[1]

    Cell Line: 3T3-L1 adipocytes
    Concentration: 200 μM with 1.15 nM and 2.87 nM TNFα
    Incubation Time: 72 h
    Result: TNFα reduced expression of GLUT4 and adiponectin, and increased SOCS3 protein levels in these cells. And these effects were prevented.

    Cell Invasion Assay[2]

    Cell Line: HeLa and SiHa cells
    Concentration: 500 μM
    Incubation Time: 72 h
    Result: Significantly reduced hypoxia-elicited cell invasion in both cell models.

    Cell Migration Assay [2]

    Cell Line: HeLa and SiHa cells
    Concentration: 500 μM
    Incubation Time: 72 h
    Result: Decreased hypoxia-induced migration from 180 and 240% to 60 and 70% in HeLa and SiHa cells, respectively.

    Western Blot Analysis[2]

    Cell Line: HeLa and SiHa cells
    Concentration: 500 μM
    Incubation Time: 72 h
    Result: Effectively prevented hypoxia-induced downregulation of E-cadherin and strongly inhibited hypoxia-induced upregulation of α-SMA and vimentin.
    In Vivo

    β-Aminopropionitrile (BAPN) (100 mg/kg/day; p.o.; 6 weeks) reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats[1].
    β-Aminopropionitrile monofumarate (1 g/kg/day; p.o.; 4 weeks) induces thoracic aortic dissection in C57BL/6 mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats of 150 g, high-fat diet (HFD) model[1]
    Dosage: 100 mg/kg/day
    Administration: In the drinking water, 6 weeks
    Result: Significantly prevented the rise in body weight in HFD rats, but not in animals that were fed a standard diet. Reduced the increase in the weight of white adipose tissue (both epididymal and lumbar) in obese animals and attenuated their enhanced adiposity. Improved fasted glucose and insulin levels and consequently reduced HOMA index in the HFD group. Improved insulin signalling in adipose tissue from obese animals.
    Animal Model: C57BL/6 mice[3]
    Dosage: 1 g/kg/day
    Administration: In the drinking water, 4 weeks
    Result: Induce thoracic aortic dissection (TAD) in all mice with 24 h of Ang II infusion. Caused 87% of C57BL/6 mice to develop TAD without Ang II.
    Molecular Weight

    106.55

    Formula

    C3H7ClN2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    NCCC#N.Cl
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (938.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 9.3853 mL 46.9263 mL 93.8527 mL
    5 mM 1.8771 mL 9.3853 mL 18.7705 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (23.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (23.46 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 9.3853 mL 46.9263 mL 93.8526 mL 234.6316 mL
    5 mM 1.8771 mL 9.3853 mL 18.7705 mL 46.9263 mL
    10 mM 0.9385 mL 4.6926 mL 9.3853 mL 23.4632 mL
    15 mM 0.6257 mL 3.1284 mL 6.2568 mL 15.6421 mL
    20 mM 0.4693 mL 2.3463 mL 4.6926 mL 11.7316 mL
    25 mM 0.3754 mL 1.8771 mL 3.7541 mL 9.3853 mL
    30 mM 0.3128 mL 1.5642 mL 3.1284 mL 7.8211 mL
    40 mM 0.2346 mL 1.1732 mL 2.3463 mL 5.8658 mL
    50 mM 0.1877 mL 0.9385 mL 1.8771 mL 4.6926 mL
    60 mM 0.1564 mL 0.7821 mL 1.5642 mL 3.9105 mL
    80 mM 0.1173 mL 0.5866 mL 1.1732 mL 2.9329 mL
    100 mM 0.0939 mL 0.4693 mL 0.9385 mL 2.3463 mL
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    β-Aminopropionitrile hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    β-Aminopropionitrile646-03-7BAPNMonoamine OxidaseEndogenous MetaboliteMAOthoracic aortic dissectionGLUT4insulincervical carcinoma cellsHeLaSiHaobesitylysyl oxidaseInhibitorinhibitorinhibit

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