1. Neuronal Signaling Metabolic Enzyme/Protease
  2. Monoamine Oxidase Endogenous Metabolite
  3. β-Aminopropionitrile

β-Aminopropionitrile  (Synonyms: BAPN)

Cat. No.: HY-Y1750 Purity: 99.94%
COA Handling Instructions

β-Aminopropionitrile (BAPN) is a specific, irreversible and orally active lysyl oxidase (LOX) inhibitor. β-Aminopropionitrile targets the active site of LOX or LOXL isoenzymes.

For research use only. We do not sell to patients.

β-Aminopropionitrile Chemical Structure

β-Aminopropionitrile Chemical Structure

CAS No. : 151-18-8

Size Price Stock Quantity
Liquid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Liquid
500 mg USD 55 In-stock
1 g   Get quote  
5 g   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

β-Aminopropionitrile (BAPN) is a specific, irreversible and orally active lysyl oxidase (LOX) inhibitor. β-Aminopropionitrile targets the active site of LOX or LOXL isoenzymes[1][2].

IC50 & Target

Lysyl Oxidase

 

Human Endogenous Metabolite

 

In Vitro

β-Aminopropionitrile (BAPN) normalizes the expression of GLUT4 and adiponectin, and improves glucose uptake in an in vitro model of insulin resistance[1].
β-Aminopropionitrile (500 μM; 72 h) blocks the hypoxia-induced EMT morphological and marker protein changes, and inhibits invasion and migration capacities of cervical carcinoma cells in vitro[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: 3T3-L1 adipocytes
Concentration: 200 μM with 1.15 nM and 2.87 nM TNFα
Incubation Time: 72 h
Result: TNFα reduced expression of GLUT4 and adiponectin, and increased SOCS3 protein levels in these cells. And these effects were prevented.

Cell Invasion Assay[2]

Cell Line: HeLa and SiHa cells
Concentration: 500 μM
Incubation Time: 72 h
Result: Significantly reduced hypoxia-elicited cell invasion in both cell models.

Cell Migration Assay [2]

Cell Line: HeLa and SiHa cells
Concentration: 500 μM
Incubation Time: 72 h
Result: Decreased hypoxia-induced migration from 180 and 240% to 60 and 70% in HeLa and SiHa cells, respectively.

Western Blot Analysis[2]

Cell Line: HeLa and SiHa cells
Concentration: 500 μM
Incubation Time: 72 h
Result: Effectively prevented hypoxia-induced downregulation of E-cadherin and strongly inhibited hypoxia-induced upregulation of α-SMA and vimentin.
In Vivo

β-Aminopropionitrile (BAPN) (100 mg/kg/day; p.o.; 6 weeks) reduces body weight gain and improves the metabolic profile in diet-induced obesity in rats[1].
β-Aminopropionitrile monofumarate (1 g/kg/day; p.o.; 4 weeks) induces thoracic aortic dissection in C57BL/6 mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats of 150 g, high-fat diet (HFD) model[1]
Dosage: 100 mg/kg/day
Administration: In the drinking water, 6 weeks
Result: Significantly prevented the rise in body weight in HFD rats, but not in animals that were fed a standard diet. Reduced the increase in the weight of white adipose tissue (both epididymal and lumbar) in obese animals and attenuated their enhanced adiposity. Improved fasted glucose and insulin levels and consequently reduced HOMA index in the HFD group. Improved insulin signalling in adipose tissue from obese animals.
Animal Model: C57BL/6 mice[3]
Dosage: 1 g/kg/day
Administration: In the drinking water, 4 weeks
Result: Induce thoracic aortic dissection (TAD) in all mice with 24 h of Ang II infusion. Caused 87% of C57BL/6 mice to develop TAD without Ang II.
Molecular Weight

70.09

Formula

C3H6N2

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

N#CCCN

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (1426.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (713.37 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 14.2674 mL 71.3369 mL 142.6737 mL
5 mM 2.8535 mL 14.2674 mL 28.5347 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 3.25 mg/mL (46.37 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 3.25 mg/mL (46.37 mM); Clear solution

    This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (1426.74 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.99%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 14.2674 mL 71.3369 mL 142.6737 mL 356.6843 mL
5 mM 2.8535 mL 14.2674 mL 28.5347 mL 71.3369 mL
10 mM 1.4267 mL 7.1337 mL 14.2674 mL 35.6684 mL
15 mM 0.9512 mL 4.7558 mL 9.5116 mL 23.7790 mL
20 mM 0.7134 mL 3.5668 mL 7.1337 mL 17.8342 mL
25 mM 0.5707 mL 2.8535 mL 5.7069 mL 14.2674 mL
30 mM 0.4756 mL 2.3779 mL 4.7558 mL 11.8895 mL
40 mM 0.3567 mL 1.7834 mL 3.5668 mL 8.9171 mL
50 mM 0.2853 mL 1.4267 mL 2.8535 mL 7.1337 mL
60 mM 0.2378 mL 1.1889 mL 2.3779 mL 5.9447 mL
80 mM 0.1783 mL 0.8917 mL 1.7834 mL 4.4586 mL
100 mM 0.1427 mL 0.7134 mL 1.4267 mL 3.5668 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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β-Aminopropionitrile
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