20:4 Lyso PI 

20:4 Lyso PI acts as an activator of GPR55 and RhoA. 20:4 Lyso PI activates the GPR55-RhoA-ROCK pathway, thereby inducing morphological changes, cytoskeleton assembly, cell rounding and stress fiber formation. 20:4 Lyso PI can be used in research related to diseases such as those of the nervous system^[1].

20:4 Lyso PI (2-arachidonoyl (20:4) LPI) (10 nM-100 µM; 0-30 min) induces rounding of GPR55-expressing HEK293 cells via the RhoA-ROCK pathway (EC₅₀ = 10 nM), while it has no effect on HEK293 cells transfected with empty vector^[1].
20:4 Lyso PI (1 µM; 0-20 min) activates RhoA in a time-dependent manner in HEK293 cells expressing GPR55, but exerts no effect on HEK293 cells transfected with empty vector^[1].
20:4 Lyso PI (1 µM; 5 min) induces stress fiber formation in HEK293 cells expressing GPR55, and this effect is inhibited by the ROCK inhibitor Y-27632 (HY-10071)^[1].
20:4 Lyso PI (1 µM; 5 min) activates ERK in HEK293 cells expressing GPR55, in which the MEK pathway plays a major role, while the ROCK and PKC pathways exert partial effects^[1].
1 µM 20:4 Lyso PI increases intracellular calcium levels in HEK293 cells expressing GPR55. This effect is partially mediated by the tyrosine kinase, MEK and PLC pathways, and does not involve the RhoA-ROCK pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

620.67

C₂₉H₄₉O₁₂P

1246430-04-5

O[C@H]1[C@@H](OP(O)(OC[C@@H](COC(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)=O)O)=O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nakajima K, et al. Lysophosphatidylinositol Induced Morphological Changes and Stress Fiber Formation through the GPR55-RhoA-ROCK Pathway. Int J Mol Sci. 2022;23(18):10932. Published 2022 Sep 18.  [Content Brief]