20:4 Lyso PI

20:4 Lyso PI (2-arachidonoyl (20:4) LPI) (10 nM-100 µM; 0-30 min) induces rounding of GPR55-expressing HEK293 cells via the RhoA-ROCK pathway (EC₅₀ = 10 nM), while it has no effect on HEK293 cells transfected with empty vector^[1].
20:4 Lyso PI (1 µM; 0-20 min) activates RhoA in a time-dependent manner in HEK293 cells expressing GPR55, but exerts no effect on HEK293 cells transfected with empty vector^[1].
20:4 Lyso PI (1 µM; 5 min) induces stress fiber formation in HEK293 cells expressing GPR55, and this effect is inhibited by the ROCK inhibitor Y-27632 (HY-10071)^[1].
20:4 Lyso PI (1 µM; 5 min) activates ERK in HEK293 cells expressing GPR55, in which the MEK pathway plays a major role, while the ROCK and PKC pathways exert partial effects^[1].
1 µM 20:4 Lyso PI increases intracellular calcium levels in HEK293 cells expressing GPR55. This effect is partially mediated by the tyrosine kinase, MEK and PLC pathways, and does not involve the RhoA-ROCK pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.