Cat. No.: HY-113341

7β-Hydroxycholesterol

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

7β-Hydroxycholesterol is an oxysterol that derived by the oxidation of cholesterol. 7β-hydroxycholesterol can induce cellular oxidative stress, apoptosis, and necrosis, resulting in cytotoxicity. 7β-hydroxycholesterol has antitumor activity.

For research use only. We do not sell to patients.

Purity: 99.72%
CAS No.: 566-27-8
Formula: C₂₇H₄₆O₂
Molecular Weight:402.65

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

7β-Hydroxycholesterol Chemical Structure

CAS No.: 566-27-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

IL-1β

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	13.6 μM Compound: 6	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
A549	IC₅₀	13.6 μM Compound: 8	Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
ARPE-19	IC₅₀	21.6 μM Compound: 6	Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
ARPE-19	IC₅₀	21.6 μM Compound: 8	Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
BJ	IC₅₀	20 μM Compound: 8	Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
BJ	IC₅₀	20.1 μM Compound: 3	Cytotoxicity against human BJ cells after 48 hrs by Alamar Blue assay Cytotoxicity against human BJ cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
BJ	IC₅₀	20.1 μM Compound: 6	Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
C6	IC₅₀	15 μM Compound: 7beta-OHC	Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method	[PMID: 24211631]
HEK293	EC₅₀	10 μM Compound: 5	Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis	[PMID: 23830695]
HEK293	EC₅₀	3.4 μM Compound: 10	Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy	[PMID: 24928400]
HepG2	IC₅₀	14.6 μM Compound: 6	Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HepG2	IC₅₀	14.6 μM Compound: 8	Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HMEC-1	IC₅₀	19.1 μM Compound: 8	Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HT-29	IC₅₀	6.4 μM Compound: 3	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
HT-29	IC₅₀	6.9 μM Compound: 6	Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
HT-29	IC₅₀	6.9 μM Compound: 8	Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
HT-29	IC₅₀	7.2 μM Compound: 8	Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay	[PMID: 20931970]
LAMA-84	IC₅₀	4.3 μM Compound: 6	Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
LAMA-84	IC₅₀	4.3 μM Compound: 8	Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
LAMA-84	IC₅₀	4.8 μM Compound: 3	Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar Blue assay Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar Blue assay	[PMID: 19473028]
MCF7	IC₅₀	8.7 μM Compound: 6	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
MCF7	IC₅₀	8.7 μM Compound: 8	Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
PC-3	IC₅₀	21.9 μM Compound: 8	Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay	[PMID: 20931970]
PC-3	IC₅₀	22 μM Compound: 6	Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]
SH-SY5Y	IC₅₀	16.4 μM Compound: 6	Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay	[PMID: 21797280]

In Vitro

7β-Hydroxycholesterol (20-40 μg/mL; 20-48 h) induces apoptosis, adhesion molecule expression and stimulates IL-1β secretion in human umbilical vein endothelial cells (HUVECs)^[1].
7β-Hydroxycholesterol (5-80 μg/mL; 48 h) has a double cytotoxic effect on vascular wall cells by inducing apoptosis of endothelial and smooth muscle cells and fibroblast necrosis^[2].
7β-Hydroxycholesterol (30-60 μM; 12-96 h) inhibits the growth and induces cell apoptosis in human colon cancer cell Caco-2^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

Caco-2 cell
Concentration:

30 and 60 μM
Incubation Time:

24, 48, 72 and 96 h
Result:

Inhibited cell growth in a dose-dependent manner.

Chemical Information

CAS No. 566-27-8
Appearance Solid
Molecular Weight 402.65
Formula C₂₇H₄₆O₂
Color White to off-white
SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])[C@@H](O)C=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

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Journal Impact Factor

ACS Nano

Immune Modulatory Oxysterols Produced from Cholesterol-Containing Lipid Nanoparticles Regulate Tumor Growth. [Abstract]

2026 Mar 13. PMID: 41825961
Cell Chem Biol

Transient hydroxycholesterol treatment restrains TCR signaling to promote long-term immunity. [Abstract]

2024 May 16;31(5):920-931.e6. PMID: 38759618

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (248.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4835 mL

12.4177 mL

24.8355 mL

5 mM

0.4967 mL

2.4835 mL

4.9671 mL

10 mM

0.2484 mL

1.2418 mL

2.4835 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lemaire S, et al. Different patterns of IL-1beta secretion, adhesion molecule expression and apoptosis induction in human endothelial cells treated with 7alpha-, 7beta-hydroxycholesterol, or 7-ketocholesterol. FEBS Lett. 1998 Dec 4;440(3):434-9. [Content Brief]

[2]. Lizard G, et al. Characterization and comparison of the mode of cell death, apoptosis versus necrosis, induced by 7beta-hydroxycholesterol and 7-ketocholesterol in the cells of the vascular wall. Arterioscler Thromb Vasc Biol. 1999 May;19(5):1190-200. [Content Brief]

[3]. Roussi S, et al. Different apoptotic mechanisms are involved in the antiproliferative effects of 7beta-hydroxysitosterol and 7beta-hydroxycholesterol in human colon cancer cells. Cell Death Differ. 2005 Feb;12(2):128-35. [Content Brief]

[4]. Anne Vejux, et al. 7-Ketocholesterol and 7β-hydroxycholesterol: In vitro and animal models used to characterize their activities and to identify molecules preventing their toxicity. Biochem Pharmacol. 2020 Mar;173:113648. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4835 mL	12.4177 mL	24.8355 mL	62.0887 mL
	5 mM	0.4967 mL	2.4835 mL	4.9671 mL	12.4177 mL
	10 mM	0.2484 mL	1.2418 mL	2.4835 mL	6.2089 mL
	15 mM	0.1656 mL	0.8278 mL	1.6557 mL	4.1392 mL
	20 mM	0.1242 mL	0.6209 mL	1.2418 mL	3.1044 mL
	25 mM	0.0993 mL	0.4967 mL	0.9934 mL	2.4835 mL
	30 mM	0.0828 mL	0.4139 mL	0.8278 mL	2.0696 mL
	40 mM	0.0621 mL	0.3104 mL	0.6209 mL	1.5522 mL
	50 mM	0.0497 mL	0.2484 mL	0.4967 mL	1.2418 mL
	60 mM	0.0414 mL	0.2070 mL	0.4139 mL	1.0348 mL
	80 mM	0.0310 mL	0.1552 mL	0.3104 mL	0.7761 mL
	100 mM	0.0248 mL	0.1242 mL	0.2484 mL	0.6209 mL

Keywords

7β-Hydroxycholesterol

2 Cited Publications

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7β-Hydroxycholesterol

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Lipid Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Pyroptosis Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Food-Sourced Compound Library

Human Metabolite Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) 7β-Hydroxycholesterol

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

7β-Hydroxycholesterol

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Lipid Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Pyroptosis Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Angiogenesis-Related Compound Library

Food-Sourced Compound Library

Human Metabolite Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) 7β-Hydroxycholesterol

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

7β-Hydroxycholesterol Related Classifications

Cat. No.: HY-113341