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AS1842856 

Cat. No.: HY-100596 Purity: 98.09%
Data Sheet SDS Handling Instructions

AS1842856 is a potent and cell-permeable Foxo1 inhibitor with an IC50 of 30 nM.

For research use only. We do not sell to patients.
AS1842856 Chemical Structure

AS1842856 Chemical Structure

CAS No. : 836620-48-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
5 mg $50 In-stock
10 mg $70 In-stock
50 mg $240 In-stock
100 mg $440 In-stock
200 mg $740 In-stock
500 mg $1640 In-stock
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

AS1842856 is a potent and cell-permeable Foxo1 inhibitor with an IC50 of 30 nM.

IC50 & Target

IC50: 30 nM (Foxo1)[1]

In Vitro

AS1842856 potently inhibits human Foxo1 transactivation and reduces glucose production through the inhibition of glucose-6 phosphatase and phosphoenolpyruvate carboxykinase mRNA levels in a rat hepatic cell line[1].

In Vivo

Oral administration of AS1842856 to diabetic db/db mice leads to a drastic decrease in fasting plasma glucose level via the inhibition of hepatic gluconeogenic genes, whereas administration to normal mice has no effect on the fasting plasma glucose level. Treatment with AS1842856 also suppresses an increase in plasma glucose level caused by pyruvate injection in both normal and db/db mice[1].

References
Preparing Stock Solutions
Concentration Volume (DMSO) Mass 1 mg 5 mg 10 mg
1 mM 2.8787 mL 14.3935 mL 28.7869 mL
5 mM 0.5757 mL 2.8787 mL 5.7574 mL
10 mM 0.2879 mL 1.4393 mL 2.8787 mL
Cell Assay
[1]

Rat hepatoma Fao cells are cultured in DMEM with 5.5 mM glucose and 10% FBS. Glucose production rate is measured using glucose CII-test reagent. In brief, after 18 h of treatment with either insulin or AS1842856 at the indicated concentrations, the cells are ished three times with PBS. The cells are then incubated for 3 h at 37°C in 5% CO2 in a glucose production buffer (glucose-free DMEM, pH 7.4, containing 20 mM sodium pyruvate, without phenol red)[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

AS1842856 is dissolved in 6% cyclodextrin for oral administration. Pyruvate or glucose tolerance tests are performed in male mice aged 7 to 9 weeks. Mice are orally administered either AS1842856 dissolved in 6% cyclodextrin or vehicle (6% cyclodextrin only) at three time points (8 AM, 6 PM, and 8 AM on the second day). Food is removed after initial dosing and withheld throughout the study (26-h fasting)[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

347.38

Formula

C₁₈H₂₂FN₃O₃

CAS No.

836620-48-5

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 7.6 mg/mL (Need ultrasonic and warming)

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
AS1842856
Cat. No.:
HY-100596
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