1. Autophagy
  2. Autophagy
  3. AS1842856

AS1842856 

Cat. No.: HY-100596 Purity: 98.89%
Handling Instructions

AS1842856, un inhibiteur spécifique de Foxo1 (IC50=30 nM), supprime puissamment l'autophagie[1 ]. AS1842856 inhibe l'activité de FoxO1 en supprimant l'expression de SIRT1. AS1842856 ne réduit l'activité de FoxO1 qu'en se liant à lui, sans affecter sa transcription et son expression protéique.

AS1842856, a specific Foxo1 inhibitor (IC50=30 nM), potently suppresses autophagy. AS1842856 inhibits FoxO1 activity by suppressing the expression of SIRT1. AS1842856 only reduces the activity of FoxO1 by binding with it, without affecting its transcription and protein expression.

For research use only. We do not sell to patients.

AS1842856 Chemical Structure

AS1842856 Chemical Structure

CAS No. : 836620-48-5

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10 mM * 1  mL in DMSO USD 66 In-stock
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50 mg USD 190 In-stock
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100 mg USD 290 In-stock
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Based on 7 publication(s) in Google Scholar

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Description

AS1842856, a specific Foxo1 inhibitor (IC50=30 nM), potently suppresses autophagy[1]. AS1842856 inhibits FoxO1 activity by suppressing the expression of SIRT1. AS1842856 only reduces the activity of FoxO1 by binding with it, without affecting its transcription and protein expression[2].

IC50 & Target

IC50: 30 nM (Foxo1)[1]

In Vitro

AS1842856 potently inhibits human Foxo1 transactivation and reduces glucose production through the inhibition of glucose-6 phosphatase and phosphoenolpyruvate carboxykinase mRNA levels in a rat hepatic cell line[1]. After AS1842856 treatment, there is no significant difference in the protein expression of p-FoxO1 and FoxO1 compared with the control group, but the expression of p-Akt is decreased compared with the control group[2].

In Vivo

Oral administration of AS1842856 to diabetic db/db mice leads to a drastic decrease in fasting plasma glucose level via the inhibition of hepatic gluconeogenic genes, whereas administration to normal mice has no effect on the fasting plasma glucose level. Treatment with AS1842856 also suppresses an increase in plasma glucose level caused by pyruvate injection in both normal and db/db mice[1].

Molecular Weight

347.38

Formula

C₁₈H₂₂FN₃O₃

CAS No.

836620-48-5

SMILES

O=C(C1=CN(CC)C2=C(C(N)=C(F)C(NC3CCCCC3)=C2)C1=O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (14.39 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8787 mL 14.3935 mL 28.7869 mL
5 mM 0.5757 mL 2.8787 mL 5.7574 mL
10 mM 0.2879 mL 1.4393 mL 2.8787 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Rat hepatoma Fao cells are cultured in DMEM with 5.5 mM glucose and 10% FBS. Glucose production rate is measured using glucose CII-test reagent. In brief, after 18 h of treatment with AS1842856 at the indicated concentrations, the cells are ished three times with PBS. The cells are then incubated for 3 h at 37°C in 5% CO2 in a glucose production buffer (glucose-free DMEM, pH 7.4, containing 20 mM sodium pyruvate, without phenol red)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

AS1842856 is dissolved in 6% cyclodextrin for oral administration. Pyruvate or glucose tolerance tests are performed in male mice aged 7 to 9 weeks. Mice are orally administered either AS1842856 dissolved in 6% cyclodextrin or vehicle (6% cyclodextrin only) at three time points (8 AM, 6 PM, and 8 AM on the second day). Food is removed after initial dosing and withheld throughout the study (26-h fasting)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.89%

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Keywords:

AS1842856AS 1842856AS-1842856AutophagyInhibitorinhibitorinhibit

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AS1842856
Cat. No.:
HY-100596
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