1. Autophagy
  2. Autophagy
  3. AS1842856

AS1842856 

Cat. No.: HY-100596 Purity: 99.83%
COA Handling Instructions

AS1842856, un inhibiteur spécifique de Foxo1 (IC50=30 nM), supprime puissamment l'autophagie[1 ]. AS1842856 inhibe l'activité de FoxO1 en supprimant l'expression de SIRT1. AS1842856 ne réduit l'activité de FoxO1 qu'en se liant à lui, sans affecter sa transcription et son expression protéique.

AS1842856, a specific Foxo1 inhibitor (IC50=30 nM), potently suppresses autophagy. AS1842856 only reduces the activity of FoxO1 by binding with it, without affecting its transcription and protein expression.

For research use only. We do not sell to patients.

AS1842856 Chemical Structure

AS1842856 Chemical Structure

CAS No. : 836620-48-5

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 190 In-stock
Estimated Time of Arrival: December 31
100 mg USD 290 In-stock
Estimated Time of Arrival: December 31
200 mg USD 490 In-stock
Estimated Time of Arrival: December 31
500 mg USD 980 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 35 publication(s) in Google Scholar

Top Publications Citing Use of Products

34 Publications Citing Use of MCE AS1842856

WB

    AS1842856 purchased from MCE. Usage Cited in: Mol Cell Biochem. 2022 Nov 24.  [Abstract]

    AS1842856 (1 μM; 48 h) reverses CEP55 downregulation-mediated inhibition of PCNA and CDK2 levels and elevation of cl-caspase-3 expression in Ishikawa and RL95-2 cells.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    AS1842856, a specific Foxo1 inhibitor (IC50=30 nM), potently suppresses autophagy[1]. AS1842856 only reduces the activity of FoxO1 by binding with it, without affecting its transcription and protein expression[2].

    IC50 & Target

    IC50: 30 nM (Foxo1)[1]

    In Vitro

    AS1842856 potently inhibits human Foxo1 transactivation and reduces glucose production through the inhibition of glucose-6 phosphatase and phosphoenolpyruvate carboxykinase mRNA levels in a rat hepatic cell line[1]. After AS1842856 treatment, there is no significant difference in the protein expression of p-FoxO1 and FoxO1 compared with the control group, but the expression of p-Akt is decreased compared with the control group[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Oral administration of AS1842856 to diabetic db/db mice leads to a drastic decrease in fasting plasma glucose level via the inhibition of hepatic gluconeogenic genes, whereas administration to normal mice has no effect on the fasting plasma glucose level. Treatment with AS1842856 also suppresses an increase in plasma glucose level caused by pyruvate injection in both normal and db/db mice[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    347.38

    Formula

    C18H22FN3O3

    CAS No.
    SMILES

    O=C(C1=CN(CC)C2=C(C(N)=C(F)C(NC3CCCCC3)=C2)C1=O)O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 5 mg/mL (14.39 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8787 mL 14.3935 mL 28.7869 mL
    5 mM 0.5757 mL 2.8787 mL 5.7574 mL
    10 mM 0.2879 mL 1.4393 mL 2.8787 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  6% HP-β-CD in saline

      Solubility: 10 mg/mL (28.79 mM); Suspension solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 1.67 mg/mL (4.81 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 1 mg/mL (2.88 mM); Suspended solution; Need ultrasonic

    • 4.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 1 mg/mL (2.88 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.83%

    References
    Cell Assay
    [1]

    Rat hepatoma Fao cells are cultured in DMEM with 5.5 mM glucose and 10% FBS. Glucose production rate is measured using glucose CII-test reagent. In brief, after 18 h of treatment with AS1842856 at the indicated concentrations, the cells are ished three times with PBS. The cells are then incubated for 3 h at 37°C in 5% CO2 in a glucose production buffer (glucose-free DMEM, pH 7.4, containing 20 mM sodium pyruvate, without phenol red)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    AS1842856 is dissolved in 6% cyclodextrin for oral administration. Pyruvate or glucose tolerance tests are performed in male mice aged 7 to 9 weeks. Mice are orally administered either AS1842856 dissolved in 6% cyclodextrin or vehicle (6% cyclodextrin only) at three time points (8 AM, 6 PM, and 8 AM on the second day). Food is removed after initial dosing and withheld throughout the study (26-h fasting)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    AS1842856
    Cat. No.:
    HY-100596
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