Cat. No.: HY-N0076

Bilobalide

(Synonyms: (-)-Bilobalide)

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Bilobalide, a sesquiterpene trilactone constituent of Ginkgo biloba, inhibits the NMDA-induced efflux of choline with an IC₅₀ value of 2.3 μM. Bilobalide prevents apoptosis through activation of the PI3K/Akt pathway in SH-SY5Y cells. Exerts protective and trophic effects on neurons.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 33570-04-6
Formula: C₁₅H₁₈O₈
Molecular Weight:326.30

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Bilobalide Chemical Structure

CAS No.: 33570-04-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Bilobalide (1-100 μM) completely suppresses the NMDA-evoked release of choline in a concentration-dependent manner with IC₅₀ value of 2.3 μM^[1].
Bilobalide (1, 5 and 10 μM) alone for 24 h does not affect cell viability of SH-SY5Y cells. Pre-treatment of cells with Bilobalide concentration-dependently prevents Aβ 1-42-, H₂O₂- and serum deprivation-induced decrease of cell viability, with the best protective effect obtained at 10 μM^[2].
Bilobalide (5 and 10 μM; 24 h) treatment dose-dependently increases levels of p-Akt (Ser473 and Thr308) in SH-SY5Y cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:

SH-SY5Y cells
Concentration:

5 and 10 μM
Incubation Time:

24 hours
Result:

Induced a significant increase in levels of p-Akt (Ser473 and Thr308).

In Vivo

Bilobalide (20 mg/kg) completely suppresses the NMDA-induced release of choline in vivo while basal choline levels were not significantly affected. NMDA causes a release of choline in vivo when infused into the hippocampus of freely moving rats by retrograde dialysis. Bilobalide (20 mg/kg i.p.) completely inhibits the effect induced by NMDA^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar rats (250-350 g)^[1]
Dosage:

20 mg/kg
Administration:

I.p. injection 60 min before NMDA infusion
Result:

Lowered basal choline efflux only slightly (by 7%) but fully antagonized the NMDA-induced increase of choline release. The convulsive effect of NMDA was almost completely suppressed.

Chemical Information

CAS No. 33570-04-6
Appearance Solid
Molecular Weight 326.30
Formula C₁₅H₁₈O₈
Color White to off-white
SMILES

O=C(O1)C[C@@]2([C@]1([H])C[C@]3(C(C)(C)C)O)[C@@]34[C@](OC([C@@H]4O)=O)([H])OC2=O
Synonyms

(-)-Bilobalide
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

All RT-PCRWBIFCell Proliferation/Viability AssayELISA

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Journal Impact Factor

Eur J Cancer Care (Engl)

2024 Apr 4.

Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

Bilobalide (1, 3, 10 μM) signifcantly reduced IL-10 and Arg-1 expression in a dose-dependent manner. Similar to the RT-qPCR analysis.

Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

Bilobalide (1, 3, 10 μM) signifcantly decreased the levels of M2 markers by Western blotting.

Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

Bilobalide (1, 3, 10 μM) signifcantly decreased the levels of M2 markers by immunofuorescence.

Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

The viability of the CRC organoids was signifcantly reduced in the Bilobalide (10 μM)-treated group compared to the blank or M2 groups.

Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

Bilobalide (10 μM) treatment (Bil + M2 group) caused a decrease and increase in IL-10 and TNF-α concentration, respectively by ELISA.
Research Square Preprint

2021 Aug.

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (306.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0647 mL

15.3233 mL

30.6466 mL

5 mM

0.6129 mL

3.0647 mL

6.1293 mL

10 mM

0.3065 mL

1.5323 mL

3.0647 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. O Weichel, et al. Bilobalide, a constituent of Ginkgo biloba, inhibits NMDA-induced phospholipase A2 activation and phospholipid breakdown in rat hippocampus. Naunyn Schmiedebergs Arch Pharmacol. 1999 Dec;360(6):609-15. [Content Brief]

[2]. Chun Shi, et al. Bilobalide prevents apoptosis through activation of the PI3K/Akt pathway in SH-SY5Y cells. Apoptosis. 2010 Jun;15(6):715-27. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0647 mL	15.3233 mL	30.6466 mL	76.6166 mL
	5 mM	0.6129 mL	3.0647 mL	6.1293 mL	15.3233 mL
	10 mM	0.3065 mL	1.5323 mL	3.0647 mL	7.6617 mL
	15 mM	0.2043 mL	1.0216 mL	2.0431 mL	5.1078 mL
	20 mM	0.1532 mL	0.7662 mL	1.5323 mL	3.8308 mL
	25 mM	0.1226 mL	0.6129 mL	1.2259 mL	3.0647 mL
	30 mM	0.1022 mL	0.5108 mL	1.0216 mL	2.5539 mL
	40 mM	0.0766 mL	0.3831 mL	0.7662 mL	1.9154 mL
	50 mM	0.0613 mL	0.3065 mL	0.6129 mL	1.5323 mL
	60 mM	0.0511 mL	0.2554 mL	0.5108 mL	1.2769 mL
	80 mM	0.0383 mL	0.1915 mL	0.3831 mL	0.9577 mL
	100 mM	0.0306 mL	0.1532 mL	0.3065 mL	0.7662 mL

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Bilobalide

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Publications Citing Use of MedChemExpress (MCE) Bilobalide

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Bilobalide Related Classifications

Cat. No.: HY-N0076