2. Apoptosis Autophagy Metabolic Enzyme/Protease
  3. Apoptosis Autophagy Endogenous Metabolite
  4. Bilobalide

Bilobalide, a sesquiterpene trilactone constituent of Ginkgo biloba, inhibits the NMDA-induced efflux of choline with an IC50 value of 2.3 μM. Bilobalide prevents apoptosis through activation of the PI3K/Akt pathway in SH-SY5Y cells. Exerts protective and trophic effects on neurons.

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No. CAS : 33570-04-6

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10 mM * 1 mL in DMSO
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Based on 2 publication(s) in Google Scholar

Other Forms of Bilobalide:

Bilobalide Related Antibodies

Top Publications Citing Use of Products
RT-PCR
WB
IF
Cell Proliferation/Viability Assay
ELISA

    Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

    Bilobalide (1, 3, 10 μM) signifcantly reduced IL-10 and Arg-1 expression in a dose-dependent manner. Similar to the RT-qPCR analysis.

    Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

    Bilobalide (1, 3, 10 μM) signifcantly decreased the levels of M2 markers by Western blotting.

    Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

    Bilobalide (1, 3, 10 μM) signifcantly decreased the levels of M2 markers by immunofuorescence.

    Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

    The viability of the CRC organoids was signifcantly reduced in the Bilobalide (10 μM)-treated group compared to the blank or M2 groups.

    Bilobalide purchased from MedChemExpress. Usage Cited in: Eur J Cancer Care (Engl). 2024 Apr 4.

    Bilobalide (10 μM) treatment (Bil + M2 group) caused a decrease and increase in IL-10 and TNF-α concentration, respectively by ELISA.

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    Descripciòn

    Bilobalide, a sesquiterpene trilactone constituent of Ginkgo biloba, inhibits the NMDA-induced efflux of choline with an IC50 value of 2.3 μM. Bilobalide prevents apoptosis through activation of the PI3K/Akt pathway in SH-SY5Y cells. Exerts protective and trophic effects on neurons[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Bilobalide (1-100 μM) completely suppresses the NMDA-evoked release of choline in a concentration-dependent manner with IC50 value of 2.3 μM[1].
    Bilobalide (1, 5 and 10 μM) alone for 24 h does not affect cell viability of SH-SY5Y cells. Pre-treatment of cells with Bilobalide concentration-dependently prevents Aβ 1-42-, H2O2- and serum deprivation-induced decrease of cell viability, with the best protective effect obtained at 10 μM[2].
    Bilobalide (5 and 10 μM; 24 h) treatment dose-dependently increases levels of p-Akt (Ser473 and Thr308) in SH-SY5Y cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Bilobalide Related Antibodies

    Western Blot Analysis

    Cell Line: SH-SY5Y cells
    Concentration: 5 and 10 μM
    Incubation Time: 24 hours
    Result: Induced a significant increase in levels of p-Akt (Ser473 and Thr308).
    In Vivo

    Bilobalide (20 mg/kg) completely suppresses the NMDA-induced release of choline in vivo while basal choline levels were not significantly affected. NMDA causes a release of choline in vivo when infused into the hippocampus of freely moving rats by retrograde dialysis. Bilobalide (20 mg/kg i.p.) completely inhibits the effect induced by NMDA[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats (250-350 g)[1]
    Dosage: 20 mg/kg
    Administration: I.p. injection 60 min before NMDA infusion
    Result: Lowered basal choline efflux only slightly (by 7%) but fully antagonized the NMDA-induced increase of choline release. The convulsive effect of NMDA was almost completely suppressed.
    Peso molecular

    326.30

    Fòrmula

    C15H18O8
    No. CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O1)C[C@@]2([C@]1([H])C[C@]3(C(C)(C)C)O)[C@@]34[C@](OC([C@@H]4O)=O)([H])OC2=O
    Structure Classification
    Initial Source
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvente y solubilidad
    In Vitro: 

    DMSO : ≥ 100 mg/mL (306.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0647 mL 15.3233 mL 30.6466 mL
    5 mM 0.6129 mL 3.0647 mL 6.1293 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
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    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.37 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureza y Documentación

    Purity: 99.92%

    SDS (393 KB)

    COA (261 KB) HNMR (269 KB) LCMS (89 KB)

    Instrucciones de manejo (2659 KB)
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.0647 mL 15.3233 mL 30.6466 mL 76.6166 mL
    5 mM 0.6129 mL 3.0647 mL 6.1293 mL 15.3233 mL
    10 mM 0.3065 mL 1.5323 mL 3.0647 mL 7.6617 mL
    15 mM 0.2043 mL 1.0216 mL 2.0431 mL 5.1078 mL
    20 mM 0.1532 mL 0.7662 mL 1.5323 mL 3.8308 mL
    25 mM 0.1226 mL 0.6129 mL 1.2259 mL 3.0647 mL
    30 mM 0.1022 mL 0.5108 mL 1.0216 mL 2.5539 mL
    40 mM 0.0766 mL 0.3831 mL 0.7662 mL 1.9154 mL
    50 mM 0.0613 mL 0.3065 mL 0.6129 mL 1.5323 mL
    60 mM 0.0511 mL 0.2554 mL 0.5108 mL 1.2769 mL
    80 mM 0.0383 mL 0.1915 mL 0.3831 mL 0.9577 mL
    100 mM 0.0306 mL 0.1532 mL 0.3065 mL 0.7662 mL
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    Bilobalide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Bilobalide33570-04-6(-)-BilobalideApoptosisAutophagyEndogenous MetaboliteSesquiterpenetrilactoneGinkgobilobaNMDAcholine apoptosisSH-SY5Yprotectivetrophic neuronsInhibitorinhibitorinhibit

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