2. PI3K/Akt/mTOR
  3. Akt
  4. CTX-0294885 hydrochloride

CTX-0294885 hydrochloride 

Cat. No.: HY-15985A Purity: 99.60%
COA Handling Instructions

CTX-0294885 hydrochloride is a broad spectrum kinase inhibitor that can capture 235 kinases from MDA-MB-231 cells, and can capture all members of the AKT family. CTX-0294885 hydrochloride is a powerful reagent for analysis of kinome signaling networks that can be used for the research of diseases like inflammation, diabetes, and cancer.

For research use only. We do not sell to patients.

CTX-0294885 hydrochloride Chemical Structure

CTX-0294885 hydrochloride Chemical Structure

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 113 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 113 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 108 In-stock
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Estimated Time of Arrival: December 31
50 mg USD 540 In-stock
Estimated Time of Arrival: December 31
100 mg USD 780 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of CTX-0294885 hydrochloride:

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

CTX-0294885 hydrochloride is a broad spectrum kinase inhibitor that can capture 235 kinases from MDA-MB-231 cells, and can capture all members of the AKT family. CTX-0294885 hydrochloride is a powerful reagent for analysis of kinome signaling networks that can be used for the research of diseases like inflammation, diabetes, and cancer[1].

In Vitro

CTX-0294885 hydrochloride is a kinase capture tool in large-scale kinome profiling experiments, with 185 kinases identified as total protein, and 179 identified from TiO2 enrichment for phosphopeptides in MDA-MB-231 cells (by quantitative MS)[1].
CTX-0294885 hydrochloridecaptures all members of the AKT family that's not identified from previous studies using other kinase capture reagents[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

474.39

Appearance

Solid

Formula

C22H25Cl2N7O
SMILES

[H]Cl.O=C(NC)C1=CC=CC=C1NC2=NC(NC3=CC=C(N4CCNCC4)C=C3)=NC=C2Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

H2O : 55 mg/mL (115.94 mM; Need ultrasonic)

DMSO : 9.17 mg/mL (19.33 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1080 mL 10.5399 mL 21.0797 mL
5 mM 0.4216 mL 2.1080 mL 4.2159 mL
10 mM 0.2108 mL 1.0540 mL 2.1080 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (105.40 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
Kinase Assay
[1]

Reactions contains assay buffer, a Nterminally biotinylated peptide substrate, and ATP at a concentration equaling the KM of the kinase. The compounds (CTX-0294885) are added in a volume of 100 nL from an 11-point-dilution series prepared in DMSO, positive and negative control reactions receiving the same volume of DMSO without compound. The kinases are added at redetermined concentrations, generally ranging between 0.2 nd 8 nM, with the enzyme being omitted from negative ontrol reactions. The reactions are incubated for 90 min at 0 °C and stopped by adding 5 μL of Stop buffer containing streptavidin-coated donor and antiphosphotyrosine acceptor beads. Plates are incubated for 4-6 h before being read on a plate reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • Dilution Calculator

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

CTX-0294885CTX0294885CTX 0294885AktPKBProtein kinase BAKTcancerkinome profilingMDA-MB-231inflammationdiabetesInhibitorinhibitorinhibit

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