1. Others
  2. Others
  3. CTX-0294885 hydrochloride

CTX-0294885 hydrochloride 

Cat. No.: HY-15985A Purity: 99.93%
Handling Instructions

CTX-0294885 hydrochloride is a sepharose-supported kinase capture reagent.

For research use only. We do not sell to patients.

CTX-0294885 hydrochloride Chemical Structure

CTX-0294885 hydrochloride Chemical Structure

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 113 In-stock
Estimated Time of Arrival: December 31
5 mg USD 108 In-stock
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Estimated Time of Arrival: December 31
50 mg USD 540 In-stock
Estimated Time of Arrival: December 31
100 mg USD 780 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of CTX-0294885 hydrochloride:

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

CTX-0294885 hydrochloride is a sepharose-supported kinase capture reagent.

In Vitro

CTX-0294885 is a novel bisanilino pyrimidine exhibiting inhibitory activity against a broad range of kinases. CTx-0294885 represents a powerful new reagent for analysis of kinome signaling networks that may facilitate development of targeted therapeutic strategies. Large-scale CTx-0294885-based affinity purification followed by LC−MS/MS leads to the identification of 235 protein kinases from MDA-MB-231 cells, including all members of the AKT family that has not been previously detected by other broad-spectrum kinase inhibitors. Addition of CTx-0294885 to a mixture of three kinase inhibitors commonly used for kinase-enrichment increases the number of kinase identifications to 261. Combining CTx-0294885 with three other broad-specificity inhibitors for kinase enabled the identification of 799 highconfidence phosphosites on 183 kinases, 10% of which are localized to the activation loop, and included previously unreported phosphosites on BMP2K, MELK, HIPK2, and PRKDC[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

474.39

Formula

C₂₂H₂₅Cl₂N₇O

SMILES

[H]Cl.O=C(NC)C1=CC=CC=C1NC2=NC(NC3=CC=C(N4CCNCC4)C=C3)=NC=C2Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 55 mg/mL (115.94 mM; Need ultrasonic)

DMSO : 9.17 mg/mL (19.33 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1080 mL 10.5399 mL 21.0797 mL
5 mM 0.4216 mL 2.1080 mL 4.2159 mL
10 mM 0.2108 mL 1.0540 mL 2.1080 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.92 mg/mL (1.94 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

Reactions contains assay buffer, a Nterminally biotinylated peptide substrate, and ATP at a concentration equaling the KM of the kinase. The compounds (CTX-0294885) are added in a volume of 100 nL from an 11-point-dilution series prepared in DMSO, positive and negative control reactions receiving the same volume of DMSO without compound. The kinases are added at redetermined concentrations, generally ranging between 0.2 nd 8 nM, with the enzyme being omitted from negative ontrol reactions. The reactions are incubated for 90 min at 0 °C and stopped by adding 5 μL of Stop buffer containing streptavidin-coated donor and antiphosphotyrosine acceptor beads. Plates are incubated for 4-6 h before being read on a plate reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

CTX-0294885CTX0294885CTX 0294885OthersInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CTX-0294885 hydrochloride
Cat. No.:
HY-15985A
Quantity:
MCE Japan Authorized Agent: