Cat. No.: HY-32351

Calcifediol

(Synonyms: 25-Hydroxy Vitamin D3)

12 Cited Publications

Customer Review

Based on 12 publication(s) in Google Scholar

Calcifediol (25-hydroxy Vitamin D3) is an effective VDR ligand and VD supplement. Calcifediol is a prohormone of the vitamin D endocrine system (VDES) and is hydroxylated in the liver to produce the active form, calcitriol. Calcifediol can rapidly increase serum VD levels.

For research use only. We do not sell to patients.

Purity: 99.94%
CAS No.: 19356-17-3
Formula: C₂₇H₄₄O₂
Molecular Weight:400.64

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 1 year , -20°C, 6 months

Calcifediol Chemical Structure

CAS No.: 19356-17-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (12)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Calcifediol in either liposomes or ethanolic solution has no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells. Treatment of infected, human bronchial 16-HBE cells with Calcifediol liposomes results in a significant reduction in bacterial survival^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 19356-17-3
Appearance Solid
Molecular Weight 400.64
Formula C₂₇H₄₄O₂
Color White to off-white
SMILES

C=C(CC1)/C(C[C@H]1O)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CCCC(C)(C)O)CC3
Synonyms

25-Hydroxy Vitamin D3
Structure Classification
- Others
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (12)

All OthersWBELISACell Imaging/Staining

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Journal Impact Factor

Nat Chem Biol

Structures of the ADGRG2-Gs complex in apo and ligand-bound forms. [Abstract]

2022 Nov;18(11):1196-1203. PMID: 35982227
Proc Natl Acad Sci U S A

Progesterone activates GPR126 to promote breast cancer development via the Gi pathway. [Abstract]

2022 Apr 12;119(15):e2117004119. PMID: 35394864
J Clin Endocrinol Metab

Leveraging menstrual effluent (ME) to investigate vitamin D and human decidualization, a potential link with fertility. [Abstract]

2026 Jan 5:dgaf708. PMID: 41492807

Calcifediol purchased from MedChemExpress. Usage Cited in: J Clin Endocrinol Metab. 2026 Jan 5:dgaf708. [Abstract]

Effects of cholecalciferol and calcifediol treatment on ME-eSC decidualization. ME-eSCs were pre-treated with vehicle (Veh) vs. cholecalciferol at Veh vs. Calcifediol at 0.1 μM 4 hrs prior to decidualization (stimulated with cAMP+MPA).
Int J Mol Sci

DKS26 Alleviates Ischemia-Reperfusion Injury-Induced Acute Kidney Injury by Stabilizing Vitamin D Receptors to Inhibit the Inflammatory Pathway of NF-κB P65. [Abstract]

2025 Mar 25;26(7):2985. PMID: 40243616

Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]

Western blot detection of p-P65Ser311, P65, TNFα, MCP-1, KIM1, and NGAL expression in cells after administration of the VDR agonist Calcifediol and/or DKS26-treated mRETCs; n = 3. Calcifediol: 300 nM, DKS26: 100 μg/mL.

Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]

Effects of Calcifediol or DKS26 on the nuclear translocation of p-P65Ser311 (red) observed by laser confocal microscopy.

Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Mar 25;26(7):2985. [Abstract]

Expression of p-IKKβ, IKKβ, p-IKBα, and IKBα after treatment with the VDR agonist calcifediol or DKS26 in mRTECs; n = 3. Calcifediol: 300 nM, DKS26: 100 μg/mL.
Int J Mol Sci

Vitamin D and Its Analogues Decrease Amyloid-β (Aβ) Formation and Increase Aβ-Degradation. [Abstract]

2017 Dec 19;18(12). pii: E2764. PMID: 29257109

Calcifediol purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2017 Dec 19;18(12). pii: E2764. [Abstract]

Effect of vitamin D and analogues on Interleukin-1β level. Interleukin-1β (IL-1β) level was determined by enzyme-linked immunosorbent assay (ELISA) technique (n ≥ 3). IL-1β was analyzed in SH-SY5Y wt cells incubated with 100 nM Calcifediol or analogues compared to cells treated with the solvent control.
Front Pharmacol

Calcipotriol Inhibits NLRP3 Signal Through YAP1 Activation to Alleviate Cholestatic Liver Injury and Fibrosis. [Abstract]

2020 Mar 31;11:200. PMID: 32296329
Int Immunopharmacol

Vitamin D attenuates DNCB-induced atopic dermatitis-like skin lesions by inhibiting immune response and restoring skin barrier function. [Abstract]

2023 Sep:122:110558. PMID: 37393836
Ther Adv Endocrinol Metab

The protective effect of iodide intake adjustment and 1,25(OH)2D3 supplementation in rat offspring following excess iodide intake. [Abstract]

2020 Oct 20:11:2042018820958295. PMID: 35154633
Virol Sin

Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]

2024 Aug 19:S1995-820X(24)00134-2. PMID: 39168248
Sci Rep

Quantitative NMR (qNMR) spectroscopy based investigation of the absolute content, stability and isomerization of 25-hydroxyvitamin D2/D3 and 24(R),25-dihydroxyvitamin D2 in solution phase. [Abstract]

2022 Feb 22;12(1):3014. PMID: 35194108
J Sep Sci

Rapid Determination of 25-Hydroxyvitamin D3 and 24,25-Dihydroxyvitamin D3 in Human Urine Based on Polystyrene/Graphene Oxide Packed-Fibers Solid-Phase Extraction Coupled With High-Performance Liquid Chromatography and Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry. [Abstract]

2025 Apr;48(4):e70130. PMID: 40219819
Biochem Biophys Res Commun

MASH associated lipotoxicity perturbs the expression of hepatic vitamin D bioactivating gene CYP2R1 in humans and mice. [Abstract]

2026 Jul 2:820:153865. PMID: 42061004

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (249.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4960 mL

12.4800 mL

24.9601 mL

5 mM

0.4992 mL

2.4960 mL

4.9920 mL

10 mM

0.2496 mL

1.2480 mL

2.4960 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.24 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Castoldi A, et al. Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. Eur J Pharm Biopharm. 2016 Nov 22. [Content Brief]

[2]. Wei Zheng, et al. Vitamin D-induced vitamin D receptor expression induces tamoxifen sensitivity in MCF-7 stem cells via suppression of Wnt/β-catenin signaling. Biosci Rep. 2018 Dec 7;38(6):BSR20180595. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4960 mL	12.4800 mL	24.9601 mL	62.4002 mL
	5 mM	0.4992 mL	2.4960 mL	4.9920 mL	12.4800 mL
	10 mM	0.2496 mL	1.2480 mL	2.4960 mL	6.2400 mL
	15 mM	0.1664 mL	0.8320 mL	1.6640 mL	4.1600 mL
	20 mM	0.1248 mL	0.6240 mL	1.2480 mL	3.1200 mL
	25 mM	0.0998 mL	0.4992 mL	0.9984 mL	2.4960 mL
	30 mM	0.0832 mL	0.4160 mL	0.8320 mL	2.0800 mL
	40 mM	0.0624 mL	0.3120 mL	0.6240 mL	1.5600 mL
	50 mM	0.0499 mL	0.2496 mL	0.4992 mL	1.2480 mL
	60 mM	0.0416 mL	0.2080 mL	0.4160 mL	1.0400 mL
	80 mM	0.0312 mL	0.1560 mL	0.3120 mL	0.7800 mL
	100 mM	0.0250 mL	0.1248 mL	0.2496 mL	0.6240 mL

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Calcifediol

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Publications Citing Use of MedChemExpress (MCE) Calcifediol

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Calcifediol

MCE Bioactive Compound Library

Natural Product Library Plus

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Publications Citing Use of MedChemExpress (MCE) Calcifediol

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (12)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Calcifediol Related Classifications

Cat. No.: HY-32351