Cinacalcet hydrochloride
Based on 17 publication(s) in Google Scholar
Cinacalcet hydrochloride (AMG-073 hydrochloride) is an orally active, allosteric agonist of Ca receptor (CaR), used for cardiovascular disease treatment.
For research use only. We do not sell to patients.
- Purity: 99.88%
- CAS No.: 364782-34-3
- Formula: C22H23ClF3N
- Molecular Weight:393.87
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
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Publications Citing Use of MedChemExpress (MCE) Cinacalcet hydrochloride
More- Adv Mater. 2024 Jun;36(24):e2311760. [Abstract]
- J Med Chem. 2015 Apr 9;58(7):2958-2966. [Abstract]
- J Agric Food Chem. 2021 Nov 17;69(45):13460-13473. [Abstract]
- Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]
- Cell Biol Toxicol. 2023 Oct;39(5):1-17. [Abstract]
- Acta Physiol. 2023 Apr;237(4):e13926. [Abstract]
- J Clin Endocrinol Metab. 2025 Jun 17;110(7):1865-1880. [Abstract]
- iScience. 2023 Mar 11;26(4):106378. [Abstract]
- J Food Sci. 2018 Sep;83(9):2394-2401. [Abstract]
- J Biol Chem. 2016 Oct 28;291(44):23126-23135. [Abstract]
- J Funct Foods. 2025 Apr.
- Calcif Tissue Int. 2023 Apr;112(4):452-462. [Abstract]
- Gen Comp Endocrinol. 2018 Oct 1:267:1-8. [Abstract]
- bioRxiv. 2025 Sep 21.
- Patent. US20230147129A1.
- Oxid Med Cell Longev. 2021 Jan 12:2021:3010548. [Abstract]
- Oxid Med Cell Longev. 2017:2017:3869561. [Abstract]
Customer Validation & Images
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Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 364782-34-3
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Appearance Solid
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Molecular Weight 393.87
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Formula C22H23ClF3N
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Color White to off-white
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SMILES
FC(F)(C1=CC(CCCN[C@@H](C2=C3C=CC=CC3=CC=C2)C)=CC=C1)F.Cl
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Synonyms
AMG-073 hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (17)
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Journal Impact Factor
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Most Recent
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Adv Mater
Efficient Delivery of Lomitapide using Hybrid Membrane-Coated Tetrahedral DNA Nanostructures for Glioblastoma Therapy. [Abstract]2024 Jun;36(24):e2311760. PMID: 38569065 -
J Med Chem
Ensemble-based virtual screening for cannabinoid-like potentiators of the human glycine receptor α1 for the treatment of pain. [Abstract]2015 Apr 9;58(7):2958-2966. PMID: 25790278 -
J Agric Food Chem
Tryptophan Promotes Intestinal Immune Defense through Calcium-Sensing Receptor (CaSR)-Dependent Metabolic Pathways. [Abstract]2021 Nov 17;69(45):13460-13473. PMID: 34748328 -
Cell Rep Methods
RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]2023 Oct 23;3(10):100599. PMID: 37797618 -
Cell Biol Toxicol
Neurorescuing effect of Cinacalcet against hypercalcemia-induced nerve injury in chronic kidney disease via TRAF2/cIAP1/KLF2/SERPINA3 signal axis. [Abstract]2023 Oct;39(5):1-17. PMID: 35635602 -
Acta Physiol
2023 Apr;237(4):e13926. PMID: 36606511 -
J Clin Endocrinol Metab
Heterogenous Origins of Calcium Homeostasis Disorders Arising from Five Heterozygous Calcium-Sensing Receptor Variants. [Abstract]2025 Jun 17;110(7):1865-1880. PMID: 39413244 -
iScience
Cinacalcet exhibits rapid bactericidal and efficient anti-biofilm activities against multidrug-resistant Gram-positive pathogens. [Abstract]2023 Mar 11;26(4):106378. PMID: 37034999 -
J Food Sci
Sensing of L-Arginine by Gut-Expressed Calcium Sensing Receptor Stimulates Gut Satiety Hormones Cholecystokinin and Glucose-Dependent Insulinotropic Peptide Secretion in Pig Model. [Abstract]2018 Sep;83(9):2394-2401. PMID: 30088839 -
J Biol Chem
Positive Allosteric Modulation of the Calcium-sensing Receptor by Physiological Concentrations of Glucose. [Abstract]2016 Oct 28;291(44):23126-23135. PMID: 27613866 -
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Calcif Tissue Int
Rescue of High Glucose Impairment of Cultured Human Osteoblasts Using Cinacalcet and Parathyroid Hormone. [Abstract]2023 Apr;112(4):452-462. PMID: 36754901
Cinacalcet hydrochloride purchased from MedChemExpress. Usage Cited in: Calcif Tissue Int. 2023 Apr;112(4):452-462. [Abstract]
Cinacalcet (CN) and parathyroid hormone (PTH), used together or separately at physiological glucose levels, produce an increase expression of RUNX2 in HOBs.
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Gen Comp Endocrinol
Phenylalanine and tryptophan stimulate gastrin and somatostatin secretion and H+-K+-ATPase activity in pigs through calcium-sensing receptor. [Abstract]2018 Oct 1:267:1-8. PMID: 29782837 -
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Oxid Med Cell Longev
2021 Jan 12:2021:3010548. PMID: 33505580 -
Oxid Med Cell Longev
Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils. [Abstract]2017:2017:3869561. PMID: 29081886
Cinacalcet hydrochloride purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2017:2017:3869561. [Abstract]
N-formyl-Met-Leu-Phe (fMLP; 10 nM) are used to stimulate the cells for 2 min, and the polymorphonuclear neutrophils (PMNs) without fMLP serve as the control. NPS-2143 (100 nM), Cinacalcet (100 nM), and PDTC (100 nM) are added and incubated. Protein expression results are representative of three experiments.
Solvent & Solubility
DMSO : ≥ 50 mg/mL (126.95 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 1 mg/mL (2.54 mM; ultrasonic and warming and heat to 60°C)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.35 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
To identify apoptosis in parathyroid glands from 5/6 nephrectomized or sham rats treated with vehicle [phosphate-buffered saline (PBS)] or Cinacalcet (10 mg/kg), nuclear DNA fragmentation is measured in situ using the Apoptag System. Briefly, parathyroid gland sections from animals treated with vehicle or cinacalcet HCl are digested with 20 μg/mL proteinase K in 0.1 mol/L PBS at room temperature for 15 minutes and incubated with 3% hydrogen peroxide/methanol for 5 minutes to block endogenous peroxidase. Sections are incubated for 1 hour at 37°C with terminal deoxynucleotidyl transferase (TdT) to label exposed 3′-OH DNA ends with digoxigenin-tagged nucleotides. Digoxigenin-labeled DNA is detected by the immunoperoxidase method. Sections are developed with 3,3′-diaminobenzidine (DAB), and the nuclei of apoptotic cells are stained brown. The specificity for apoptosis is verified by negative staining when distilled water is substituted for TdT.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (614 KB)
- English - EN (614 KB)
- Français - FR (614 KB)
- Deutsch - DE (614 KB)
- Norwegian - NO (614 KB)
- Español - ES (614 KB)
- Swedish - SV (614 KB)
- Italian - IT (614 KB)
- Korean - KR (614 KB)
- Portuguese - PT (614 KB)
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Handling Instructions (2659 KB)
References
[1]. Colloton M, et al. Cinacalcet HCl attenuates parathyroid hyperplasia in a rat model of secondary hyperparathyroidism. Kidney Int. 2005 Feb;67(2):467-76. [Content Brief]
[2]. D.A. Bushinsky, et al. Effect of cinacalcet on urine calcium excretion and supersaturation in genetic hypercalciuric stone-forming rats. Kidney Int. 2006 May;69(9):1586-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 2.5389 mL | 12.6945 mL | 25.3891 mL | 63.4727 mL |
| DMSO | 5 mM | 0.5078 mL | 2.5389 mL | 5.0778 mL | 12.6945 mL |
| 10 mM | 0.2539 mL | 1.2695 mL | 2.5389 mL | 6.3473 mL | |
| 15 mM | 0.1693 mL | 0.8463 mL | 1.6926 mL | 4.2315 mL | |
| 20 mM | 0.1269 mL | 0.6347 mL | 1.2695 mL | 3.1736 mL | |
| 25 mM | 0.1016 mL | 0.5078 mL | 1.0156 mL | 2.5389 mL | |
| 30 mM | 0.0846 mL | 0.4232 mL | 0.8463 mL | 2.1158 mL | |
| 40 mM | 0.0635 mL | 0.3174 mL | 0.6347 mL | 1.5868 mL | |
| 50 mM | 0.0508 mL | 0.2539 mL | 0.5078 mL | 1.2695 mL | |
| 60 mM | 0.0423 mL | 0.2116 mL | 0.4232 mL | 1.0579 mL | |
| 80 mM | 0.0317 mL | 0.1587 mL | 0.3174 mL | 0.7934 mL | |
| 100 mM | 0.0254 mL | 0.1269 mL | 0.2539 mL | 0.6347 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Cinacalcet hydrochloride Related Classifications
HY-70037A Related Classifications
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