2. Cell Cycle/DNA Damage
  3. Topoisomerase
  4. LMP744 hydrochloride

LMP744 hydrochloride  (Synonyms: MJ-III65 hydrochloride; NSC706744 hydrochloride)

Cat. No.: HY-U00248A Purity: 99.70%
COA Handling Instructions

Molarity Calculator

LMP744 hydrochloride (MJ-III65 hydrochloride) is a DNA intercalator and Topoisomerase I (Top1) inhibitor with antitumor activity.

For research use only. We do not sell to patients.

LMP744 hydrochloride Chemical Structure

LMP744 hydrochloride Chemical Structure

CAS No. : 308246-57-3

Size Price Stock Quantity
1 mg USD 150 In-stock
5 mg USD 290 In-stock
10 mg USD 450 In-stock
50 mg USD 1100 In-stock
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of LMP744 hydrochloride:

LMP744 hydrochloride Related Antibodies

Top Publications Citing Use of Products

View All Topoisomerase Isoform Specific Products:

View All Isoforms

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

LMP744 hydrochloride (MJ-III65 hydrochloride) is a DNA intercalator and Topoisomerase I (Top1) inhibitor with antitumor activity[1].

IC50 & Target

Top1

 

In Vitro

The GI50 value of LMP744 (MJ-III-65) for NCI60 cells is 0.1 μM[2].
LMP744 (0.1-5 μM, 3 days) induces dose-dependent accumulation of cells in the S and G2 phases of the cell cycle[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

LMP744 hydrochloride Related Antibodies

Cell Cytotoxicity Assay[2]

Cell Line: P388 and P388 Top1-deficient murine leukemia cells.
Concentration: 0.1-100 μM
Incubation Time: 3 days
Result: Induced dose-dependent accumulation of cells in the S and G2 phases of the cell cycle.
In Vivo

LMP744 (MJ-III-65) (10-50 mg/kg) administered i.v. push once a week for 4 weeks in nude mice moderately actives against human A253 and FaDu tumor xenografts without significant toxicity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude mice (nu/nu, female, 20-25 g, 8-12 weeks old) transplanted with A253 and FaDu human head and neck xenografts[1].
Dosage: 10, 25, or 50 mg/kg/week, 4 weeks
Administration: I.V. push via tail vein
Result: Moderately actived against human A253 and FaDu tumor xenografts without significant toxicity.
Clinical Trial
Molecular Weight

488.92

Formula

C24H25ClN2O7
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

OCCNCCCN1C2=C(C(C3=CC(OCO4)=C4C=C23)=O)C5=CC(OC)=C(OC)C=C5C1=O.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 105 mg/mL (214.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0453 mL 10.2266 mL 20.4532 mL
5 mM 0.4091 mL 2.0453 mL 4.0906 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.70%

COA (249 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0453 mL 10.2266 mL 20.4532 mL 51.1331 mL
5 mM 0.4091 mL 2.0453 mL 4.0906 mL 10.2266 mL
10 mM 0.2045 mL 1.0227 mL 2.0453 mL 5.1133 mL
15 mM 0.1364 mL 0.6818 mL 1.3635 mL 3.4089 mL
20 mM 0.1023 mL 0.5113 mL 1.0227 mL 2.5567 mL
25 mM 0.0818 mL 0.4091 mL 0.8181 mL 2.0453 mL
30 mM 0.0682 mL 0.3409 mL 0.6818 mL 1.7044 mL
40 mM 0.0511 mL 0.2557 mL 0.5113 mL 1.2783 mL
50 mM 0.0409 mL 0.2045 mL 0.4091 mL 1.0227 mL
60 mM 0.0341 mL 0.1704 mL 0.3409 mL 0.8522 mL
80 mM 0.0256 mL 0.1278 mL 0.2557 mL 0.6392 mL
100 mM 0.0205 mL 0.1023 mL 0.2045 mL 0.5113 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

LMP744 hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LMP744308246-57-3MJ-III65NSC706744LMP 744LMP-744NSC 706744NSC-706744TopoisomeraseInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
LMP744 hydrochloride
Cat. No.:
HY-U00248A
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.