ML141 (Synonyms: CID-2950007)

Name: ML141
Brand: MedChemExpress
SKU: HY-12755
Rating: 5.0 (32 reviews)

Cat. No.: HY-12755 Purity: 99.96%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

ML141 (CID-2950007) is a potent, allosteric, selective and reversible non-competitive inhibitor of Cdc42 GTPase. ML141 inhibits Cdc42 wild type and Cdc42 Q61L mutant with EC₅₀s of 2.1 and 2.6 μM, respectively. ML141 shows low micromolar potency and selectivity against other members of the Rho family of GTPases (Rac1, Rab2, Rab7). ML141 do not show cytotoxicity in multiple cell lines.

For research use only. We do not sell to patients.

CAS No. : 71203-35-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 32 publication(s) in Google Scholar

32 Publications Citing Use of MCE ML141

IHC

Cell Imaging/Staining

Bio/Physico-chemical Assay

: ML141 purchased from MedChemExpress. Usage Cited in: Angiogenesis. 2025 Aug 20;28(4):44. [Abstract]; In the MY-1 group, cells at the wound edge extended filopodia in the direction of migration, while in the presence of ML141, filopodia formation was suppressed, and MY-1 could not reverse the inhibitory effect of ML141 (10 μM).

: ML141 purchased from MedChemExpress. Usage Cited in: Angiogenesis. 2025 Aug 20;28(4):44. [Abstract]; Treatment with ML141 reduced the primary tube length, node count, network formation in the tube formation assay and the number of migrated cells in the Transwell assay. Similarly, MY-1 could not reverse the inhibitory effect of ML141(10 μM).

: ML141 purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2024 Nov 1:deae246. [Abstract]; ML141 (10 μM)-treated primary EnSCs remained spindle-shaped fibroblast-like cells after decidualization stimulation, without an obvious change in cell size

: ML141 purchased from MedChemExpress. Usage Cited in: Hum Reprod. 2024 Nov 1:deae246. [Abstract]; ML141 (10μM) treatment hindered the upregulation of sST2 without affecting the secretion of CLU.

: ML141 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Jan 30;14(1):478. [Abstract]; Treatment with Cdc42 inhibitor ML141 (5 µM) disassembled SCVs giving rise to increased proportion of dispersive SCV in wild-type, but not in vimentin KO cells.

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Description

In Vitro

ML141 (CID-2950007) is not cytotoxic in either cell line at doses of 0.1-3 μM after treatment for 4 days. OVCA429 cells were insensitive to 10 μM compound, whereas some cytotoxicity was observed in SKOV3ip cells at this concentration after a 4-day treatment, although it did not reach statistical significance. ML141 is not cytotoxic toward Swiss 3T3 or Vero E6 cells up to 10 μM for 24 and 48 h, respectively^[1].
ML141 inhibits 3T3 fibroblast filopodia formation and inhibits ovarian cancer cell migration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ML141 (CID-2950007) (10 μM; intracerebroventricular injection) causes acute anxiety in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6J mice^[3]
Dosage:	10 µM
Administration:	Intracerebroventricular injection
Result:	Increased anxiety in mice.

Molecular Weight

407.49

Formula

C₂₂H₂₁N₃O₃S

CAS No.

71203-35-5

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(N2N=C(C3=CC=CC=C3)CC2C4=CC=C(OC)C=C4)C=C1)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (245.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4540 mL	12.2702 mL	24.5405 mL
5 mM	0.4908 mL	2.4540 mL	4.9081 mL
10 mM	0.2454 mL	1.2270 mL	2.4540 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.14 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (5.10 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (254 KB) HNMR (254 KB) RP-HPLC (246 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Hong L, et al. Characterization of a Cdc42 protein inhibitor and its use as a molecular probe. J Biol Chem. 2013 Mar 22;288(12):8531-43. [Content Brief]

[2]. Surviladze Z, et al. A Potent and Selective Inhibitor of Cdc42 GTPase. Probe Reports from the NIH Molecular Libraries Program [Content Brief]

[3]. Hanin G, et al. Competing targets of microRNA-608 affect anxiety and hypertension. Hum Mol Genet. 2014 Sep 1;23(17):4569-80. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4540 mL	12.2702 mL	24.5405 mL	61.3512 mL
	5 mM	0.4908 mL	2.4540 mL	4.9081 mL	12.2702 mL
	10 mM	0.2454 mL	1.2270 mL	2.4540 mL	6.1351 mL
	15 mM	0.1636 mL	0.8180 mL	1.6360 mL	4.0901 mL
	20 mM	0.1227 mL	0.6135 mL	1.2270 mL	3.0676 mL
	25 mM	0.0982 mL	0.4908 mL	0.9816 mL	2.4540 mL
	30 mM	0.0818 mL	0.4090 mL	0.8180 mL	2.0450 mL
	40 mM	0.0614 mL	0.3068 mL	0.6135 mL	1.5338 mL
	50 mM	0.0491 mL	0.2454 mL	0.4908 mL	1.2270 mL
	60 mM	0.0409 mL	0.2045 mL	0.4090 mL	1.0225 mL
	80 mM	0.0307 mL	0.1534 mL	0.3068 mL	0.7669 mL
	100 mM	0.0245 mL	0.1227 mL	0.2454 mL	0.6135 mL

ML141 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ML141 (Synonyms: CID-2950007)

Customer Review

ML141 Related Antibodies

32 Publications Citing Use of MCE ML141

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Ras Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

ML141 Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

ML141 Related Classifications

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