2. Autophagy
  3. LRRK2
  4. MLi-2

MLi-2, a chemical probe, is an orally active and highly selective LRRK2 inhibitor with an IC50 of 0.76 nM. MLi-2 has the potential for Parkinson’s disease.

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CAS 番号 : 1627091-47-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 198 在庫あり
Solution
10 mM * 1 mL in DMSO USD 198 在庫あり
Solid
1 mg $66 在庫あり
5 mg $180 在庫あり
10 mg $252 在庫あり
25 mg $550 在庫あり
50 mg $830 在庫あり
100 mg $1183 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

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カスタマーレビュー

Based on 12 publication(s) in Google Scholar

Other Forms of MLi-2:

MLi-2 関連抗体

Top Publications Citing Use of Products

    MLi-2 purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    U2OS cells were transfected with HaloTag-LRRK2 for 36 h. Cells were then treated with DMSO, LLOME (1 mM), or LLOME+MLi2 (1 μM, 90 min prior to LLOME incubation) for 2 h before fixation. Cells were then stained for RILPL1 and LAMP2.

    MLi-2 purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    Quantification of the RILPL1 lysosomes per cell in the three different conditions. Cells were then treated with DMSO, LLOME (1 mM), or LLOME+MLi2 (1 μM, 90 min prior to LLOME incubation) for 2 h.

    MLi-2 purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 Nov 3;224(11):e202404018.  [Abstract]

    HEK293T cells stably expressing GFP-LRRK2 and TMEM192-3xHA were seeded and treated with DMSO, LLOME (1 mM), or LLOME + MLi2 (1 μM, 90 min prior to LLOME incubation) 24 h later. Lysosomes were purified with anti-HA beads following the LYSO-IP technique. Lysosomes were then lysed, and their content was analyzed via immunoblotting.

    MLi-2 purchased from MedChemExpress. Usage Cited in: Acta Neuropathol. 2023 Aug;146(2):245-261.  [Abstract]

    Disease index score evaluation in WT and GS mice treated with placebo or MLi-2 (25 mg/kg/d in diet), after DSS administration.

    MLi-2 purchased from MedChemExpress. Usage Cited in: Acta Neuropathol. 2023 Aug;146(2):245-261.  [Abstract]

    Colonic immunophenotyping by fow cytometry of GS mice treated with MLi-2 (25 mg/kg/d in diet) or placebo, as well as WT mice, after DSS administration.

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    製品説明

    MLi-2, a chemical probe, is an orally active and highly selective LRRK2 inhibitor with an IC50 of 0.76 nM. MLi-2 has the potential for Parkinson’s disease[1].

    IC50 & Target

    IC50: 0.76 nM (LRRK2)[1]
    体外実験

    MLi-2 exhibits exceptional potency in a purified LRRK2 kinase assay in vitro( IC50=0.76 nM), a cellular assay monitoring dephosphorylation of LRRK2 pSer935 LRRK2 (IC50=1.4 nM), and a radioligand competition binding assay (IC50=3.4 nM). MLi-2 has greater than 295-fold selectivity for over 300 kinases in addition to a diverse panel of receptors and ion channels[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    MLi-2 関連抗体
    体内実験

    Acute oral and subchronic dosing in MLi-2 mice results in dose-dependent central and peripheral target inhibition over a 24-hour period as measured by dephosphorylation of pSer935 LRRK2. Treatment of MitoPark mice with MLi-2 is well tolerated over a 15-week period at brain and plasma exposures. Morphologic changes in the lung, consistent with enlarged type II pneumocytes, are observed in MLi-2-treated MitoPark mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    379.46

    分子式

    C21H25N5O2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    C[C@@](C1)([H])O[C@](C)([H])CN1C2=CC(C3=NNC4=CC=C(OC5(C)CC5)C=C43)=NC=N2
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (131.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6353 mL 13.1766 mL 26.3532 mL
    5 mM 0.5271 mL 2.6353 mL 5.2706 mL
    10 mM 0.2635 mL 1.3177 mL 2.6353 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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    一般には略語で表示されます：C1V1 = C2V2

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: 2.87 mg/mL (7.56 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.87 mg/mL (7.56 mM); Clear solution

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  30% SBE-β-CD in Saline

      Solubility: 5 mg/mL (13.18 mM); Suspension solution; Need ultrasonic

    In Vivo Dissolution Calculator
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    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.80%

    COA (252 KB) LCMS (265 KB)

    取扱説明書 (2659 KB)
    参考文献
    動物実験
    [1]

    Mice: MLi-2 is suspended in 30% Captisol and administered in a volume of 10 mL/kg. Dose calculations are on the basis of active moiety. Mice receive MLi-2 [1-100 mg/kg; by mouth (PO)], or vehicle 1 hour prior to euthanasia by excess CO2. Immediately following euthanasia, mouse brain cortex is dissected and frozen on a steel plate over dry ice for analysis of pSer935 LRRK2 via Western Blot. Plasma and brain samples are collected and frozen for determination of MLi-2 levels by LC-MS/MS[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.6353 mL 13.1766 mL 26.3532 mL 65.8831 mL
    5 mM 0.5271 mL 2.6353 mL 5.2706 mL 13.1766 mL
    10 mM 0.2635 mL 1.3177 mL 2.6353 mL 6.5883 mL
    15 mM 0.1757 mL 0.8784 mL 1.7569 mL 4.3922 mL
    20 mM 0.1318 mL 0.6588 mL 1.3177 mL 3.2942 mL
    25 mM 0.1054 mL 0.5271 mL 1.0541 mL 2.6353 mL
    30 mM 0.0878 mL 0.4392 mL 0.8784 mL 2.1961 mL
    40 mM 0.0659 mL 0.3294 mL 0.6588 mL 1.6471 mL
    50 mM 0.0527 mL 0.2635 mL 0.5271 mL 1.3177 mL
    60 mM 0.0439 mL 0.2196 mL 0.4392 mL 1.0981 mL
    80 mM 0.0329 mL 0.1647 mL 0.3294 mL 0.8235 mL
    100 mM 0.0264 mL 0.1318 mL 0.2635 mL 0.6588 mL
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    MLi-2 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    MLi-21627091-47-7MLi2MLi 2LRRK2Leucine-rich repeat kinase 2orallyParkinson’sdiseaseInhibitorinhibitorinhibit

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    製品名:
    MLi-2
    製品番号:
    HY-100411
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