1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis
  3. Madrasin

Madrasin (Synonyms: DDD00107587)

Cat. No.: HY-100236 Purity: 99.88%
Handling Instructions

Madrasin (DDD00107587) is a splicing inhibitor that prevents formation of both splicing intermediates and products in vitro and interferes with one or more early steps in the pathway of spliceosome assembly. Madrasin also can inhibit pre-mRNA splicing in vitro and modify splicing of endogenous pre-mRNA in cells.

For research use only. We do not sell to patients.

Madrasin Chemical Structure

Madrasin Chemical Structure

CAS No. : 374913-63-0

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5 mg USD 72 In-stock
Estimated Time of Arrival: December 31
10 mg USD 108 In-stock
Estimated Time of Arrival: December 31
25 mg USD 216 In-stock
Estimated Time of Arrival: December 31
50 mg USD 360 In-stock
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100 mg USD 600 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Madrasin purchased from MCE. Usage Cited in: Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254.

    Western blot shows loss of POLR2A protein in Madrasin-treated cells. Cells are harvested after Madrasin treatment (30 μM) for 24 h.

    Madrasin purchased from MCE. Usage Cited in: Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254.

    Madrasin treatment leads to decrease of spliced POLR2A transcripts and accumulation of unspliced transcripts.

    Madrasin purchased from MCE. Usage Cited in: Nucleic Acids Res. 2019 Sep 5;47(15):8239-8254.

    IF staining shows the loss of POLR2A protein after madrasin treatment. IF staining of POLR2A is performed after 24 h of Madrasin treatment at 30 uM. EU incorporation assay shows substantial reduction of newly transcribed RNA after Madrasin treatment. Assay is performed after 24 h of Madrasin treatment (30 μM).
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    Description

    Madrasin (DDD00107587) is a splicing inhibitor that prevents formation of both splicing intermediates and products in vitro and interferes with one or more early steps in the pathway of spliceosome assembly. Madrasin also can inhibit pre-mRNA splicing in vitro and modify splicing of endogenous pre-mRNA in cells[1].

    IC50 & Target

    pre-mRNA splicing[1]

    In Vitro

    Madrasin (10-30 μM; 4-24 hours; HeLa cells) treatment inhibits the splicing of each of the RIOK3, BRD2, and Hsp40, MCL1, CCNA2, AURKA and p27 pre-mRNAs in both HeLa and HEK293 cells[1].
    Madrasin (10-30 μM; 4-24 hours; HeLa and HEK293 cells) treatment shows a dose- and time-dependent inhibitory effect on cell cycle progression[1].

    RT-PCR[1]

    Cell Line: HeLa cells
    Concentration: 10 μM, 20 μM, or 30 μM
    Incubation Time: 4 hours, 8 hours, or 24 hours
    Result: Increased in inhibition of pre-mRNA splicing.

    Cell Cycle Analysis[1]

    Cell Line: HeLa and HEK293 cells
    Concentration: 10 μM, 20 μM, or 30 μM
    Incubation Time: 4 hours, 8 hours, or 24 hours
    Result: In the presence of 10 μM 8 hours after treatment, the proportion of cells in G2, M, and S phases increased, with a concomitant decrease in the number of G1 phase cells. This effect increased over time, with >40% of cells in G2 and M phase and >50% in S phase 24 hours.
    Molecular Weight

    311.34

    Formula

    C₁₆H₁₇N₅O₂

    CAS No.

    374913-63-0

    SMILES

    O=C1N=C(NC2=NC(C)=C3C=CC(OC)=CC3=N2)NC(C)=C1C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 2 mg/mL (6.42 mM; Need ultrasonic)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.2119 mL 16.0596 mL 32.1192 mL
    5 mM 0.6424 mL 3.2119 mL 6.4238 mL
    10 mM --- --- ---
    *Please refer to the solubility information to select the appropriate solvent.
    References
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    Product name:
    Madrasin
    Cat. No.:
    HY-100236
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