Meisoindigo (Synonyms: Dian III; N-Methylisoindigotin; Natura-α)

Cat. No.: HY-13680 Purity: 96.64%: FAQs
Data Sheet SDS COA Handling Instructions

Meisoindigo (Dian III), a derivative of Indirubin (HY-N0117), halts the cell cycle at the G0/G1 phase and induces apoptosis in primary acute myeloid leukemia (AML) cells. Meisoindigo exhibits high antitumor activity.

For research use only. We do not sell to patients.

Meisoindigo Chemical Structure

CAS No. : 97207-47-1

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 198	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 198	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 264	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 648	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1080	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Biological Activity
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Description

In Vitro

Meisoindigo (Dian III; 5-20 μM; for 24 hours) inhibits growth of the AML cell lines^[1].
Meisoindigo (10 μM; for 24 hours) induces apoptosis of acute myeloid leukemia^[1].
Meisoindigo (5-10 μM; for 24 hours) causes cell-cycle arrest^[1].
Meisoindigo (5-10 μM; for 24 hours) increases the cleaved caspase-3 and pro-apoptotic Bak, and decreases Bcl-2 and Bcl-xL levels in HL-60 cells^[1].
Meisoindigo (10, 30, 50, 100, 150 μM; 24 hours) interdicts LPS-induced (1 μg/mL) NLRP3 inflammasome activation and M1/M2 polarization through down-regulation of TLR4 pathways after OGD/R in HT-22 and BV2 cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HL-60, NB4, U937 leukemic cell lines
Concentration:	5, 10, 15, 20 μM
Incubation Time:	For 24 hours
Result:	Inhibited growth of the AML cell lines in a dose- and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	HL-60 cells
Concentration:	10 μM
Incubation Time:	For 24 hours
Result:	Induced apoptosis of acute myeloid leukemia.

Cell Cycle Analysis^[1]

Cell Line:	HL-60 cells
Concentration:	5, 10 μM
Incubation Time:	For 24 hours
Result:	Caused cell-cycle arrest, with more cells in sub-G1 and G0/G1 phases and fewer cells in the S phase, in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:	HL-60 cells
Concentration:	5, 10 μM
Incubation Time:	For 24 hours
Result:	Increased the cleaved caspase-3 and pro-apoptotic Bak, and decreased Bcl-2 and Bcl-xL levels in HL-60 cells.

In Vivo

Meisoindigo (Dian III; 50-150 mg/kg; IP; daily; for 14 days) has anti-leukemic activity in vivo^[1].
Meisoindigo (2, 4, 8, 12 mg/kg; IP; before MCAO and 2 h after reperfusion) significantly reduces infarct volume, ameliorates neurological deficits 3 days after middle cerebral artery occlusion (MCAO) in Wild-type C57BL/6J mice (25-30 g). Meisoindigo reduces edema and lowers AQP4 expression in the brain^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	NOD/SCID mice, 6-8 weeks old, with HL-60 leukemic cells^[1]
Dosage:	50, 100, 150 mg/kg
Administration:	IP; daily; for 14 days
Result:	Had anti-leukemic activity in vivo.

Molecular Weight

276.29

Appearance

Solid

Formula

C₁₇H₁₂N₂O₂

CAS No.

97207-47-1

SMILES

O=C1N(C)C2=C(C=CC=C2)/C1=C3C(NC4=C\3C=CC=C4)=O

Structure Classification

Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 51 mg/mL (184.59 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.6194 mL	18.0969 mL	36.1939 mL
5 mM	0.7239 mL	3.6194 mL	7.2388 mL
10 mM	0.3619 mL	1.8097 mL	3.6194 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (9.05 mM); Clear solution

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 96.64%

Select Batch:

Data Sheet (285 KB) SDS (393 KB)

COA (258 KB) LCMS (290 KB)

Handling Instructions (2659 KB)

References

[1]. Lee CC, et al. Meisoindigo is a promising agent with in vitro and in vivo activity against human acute myeloid leukemia. Leuk Lymphoma. 2010 May;51(5):897-905. [Content Brief]

[2]. Yingze Ye, et al. Meisoindigo Protects Against Focal Cerebral Ischemia-Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation and Regulating Microglia/Macrophage Polarization via TLR4/NF-κB Signaling Pathway. Front Cell Neurosci. 2019 Dec 16;13:55 [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

Meisoindigo97207-47-1Dian III N-Methylisoindigotin Natura-αApoptosisIndirubinG0/G1phaseprimaryacutemyeloidleukemiaAMLantitumorHL-60NB4U937Inhibitorinhibitorinhibit

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