IDO-IN-7 (Synonyms: NLG-919 analogue; GDC-0919 analogue)

Name: IDO-IN-7
Brand: MedChemExpress
SKU: HY-13983
Rating: 5.0 (1 reviews)

Cat. No.: HY-13983 Purity: 99.98%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

IDO-IN-7 (NLG-919 analogue) is a a potent IDO1 inhibitor with an IC₅₀ of 38 nM.

For research use only. We do not sell to patients.

IDO-IN-7 Chemical Structure

CAS No. : 1402836-58-1

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Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 131	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 131	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 76	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 119	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 317	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 554	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE IDO-IN-7

•Adv Healthc Mater. 2023 Aug 21;e2302046. [Abstract]

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IDO1 IDO2 IDO

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

IDO-IN-7 (NLG-919 analogue) is a a potent IDO1 inhibitor with an IC₅₀ of 38 nM.

IC₅₀ & Target

IDO1

38 nM (IC₅₀)

In Vitro

IDO-IN-7 (NLG-919 analogue) is a potent IDO1 inhibitor (IC₅₀=38 nM). The binding mode of IDO-IN-7 to IDO1 is experimentally available and shows a direct coordinative interaction to the sixth coordination site of ferric heme. IDO-IN-7 has been used as reference compound in other studies to validate high-throughput screening assay for IDO1 inhibition and develop immunostimulatory nanomicellar carrier^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

282.38

Formula

C₁₈H₂₂N₂O

CAS No.

1402836-58-1

Appearance

Solid

Color

White to khaki

SMILES

OC(C1CCCCC1)CC(C2=C3C=CC=C2)N4C3=CN=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (177.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 25 mg/mL (88.53 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5413 mL	17.7066 mL	35.4133 mL
5 mM	0.7083 mL	3.5413 mL	7.0827 mL
10 mM	0.3541 mL	1.7707 mL	3.5413 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 5

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 6

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (277 KB) SDS (0 KB)

COA (253 KB) LCMS (270 KB)

Handling Instructions (2659 KB)

References

[1]. Coletti A, et al. Fragment-based approach to identify IDO1 inhibitor building blocks. Eur J Med Chem. 2017 Dec 1;141:169-177. [Content Brief]

Cell Assay

IDO-IN-7 (NLG-919 analogue) and the selected fragments 8, 15, and 18 are tested in cellular assay for their ability to cross cell membrane and inhibit IDO1 catalytic activity. The cell line of murine mastocytoma P1.HTR stably transfected with murine IDO1 (P1.IDO1) is cultured for 16 hrs in the presence of each compound at the concentration of 30 mM. The ability of P1.IDO1 cells to convert LTrp contained in the culture medium at the concentration of 78.4 μM into L-Kyn is then assessed after 16 hrs of incubation^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Coletti A, et al. Fragment-based approach to identify IDO1 inhibitor building blocks. Eur J Med Chem. 2017 Dec 1;141:169-177. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.5413 mL	17.7066 mL	35.4133 mL	88.5332 mL
	5 mM	0.7083 mL	3.5413 mL	7.0827 mL	17.7066 mL
	10 mM	0.3541 mL	1.7707 mL	3.5413 mL	8.8533 mL
	15 mM	0.2361 mL	1.1804 mL	2.3609 mL	5.9022 mL
	20 mM	0.1771 mL	0.8853 mL	1.7707 mL	4.4267 mL
	25 mM	0.1417 mL	0.7083 mL	1.4165 mL	3.5413 mL
	30 mM	0.1180 mL	0.5902 mL	1.1804 mL	2.9511 mL
	40 mM	0.0885 mL	0.4427 mL	0.8853 mL	2.2133 mL
	50 mM	0.0708 mL	0.3541 mL	0.7083 mL	1.7707 mL
	60 mM	0.0590 mL	0.2951 mL	0.5902 mL	1.4756 mL
	80 mM	0.0443 mL	0.2213 mL	0.4427 mL	1.1067 mL
DMSO	100 mM	0.0354 mL	0.1771 mL	0.3541 mL	0.8853 mL

IDO-IN-7 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IDO-IN-71402836-58-1NLG-919 analogue GDC-0919 analogueIndoleamine 2,3-Dioxygenase (IDO)Inhibitorinhibitorinhibit

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IDO-IN-7 (Synonyms: NLG-919 analogue; GDC-0919 analogue)

Customer Review

IDO-IN-7 Related Antibodies

1 Publications Citing Use of MCE IDO-IN-7

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Indoleamine 2,3-Dioxygenase (IDO) Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

IDO-IN-7 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

IDO-IN-7 Related Classifications

Keywords:

Your Recently Viewed Products:

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