Oxypaeoniflorin

Name: Oxypaeoniflorin
Brand: MedChemExpress
SKU: HY-N0748
Rating: 4.5 (4 reviews)

Cat. No.: HY-N0748 Purity: 98.37%: FAQs
Data Sheet SDS COA Handling Instructions

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Oxypaeoniflorin, an anti-oxidant, is a monoterpene glycoside compound isolated from Paeoniae species. Oxypaeoniflorin has neuroprotective and anti-inflammatory effects.

For research use only. We do not sell to patients.

Oxypaeoniflorin Chemical Structure

CAS No. : 39011-91-1

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 218	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 218	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 346	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 4 publication(s) in Google Scholar

3 Publications Citing Use of MCE Oxypaeoniflorin

: Oxypaeoniflorin purchased from MedChemExpress. Usage Cited in: J Food Nutr Res. 2018, 6(1), 26-31.; LPS-induced RAW264.7 cells are pre-treated with the MCR extract (50 and 100 μg/mL), Paeoniflorin (10 and 30 μM), and Oxypaeoniflorin (10 and 30 μM) for 4 h and then co-treated with LPS (1 μg/ml) for 18 h. The expression of iNOS protein using western blot.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Oxypaeoniflorin, an anti-oxidant, is a monoterpene glycoside compound isolated from Paeoniae species. Oxypaeoniflorin has neuroprotective and anti-inflammatory effects^[1]^[2].

In Vitro

Oxypaeoniflorin (OPA; 0.1-10 µM; 8 hours) obviously reversed the hypoxia/reoxygenation (H/R)-induced decrease in cell activity and increase in apoptosis of H9c2 cells. Oxypaeoniflorin inhibits apoptosis by activating the Sirt1 (silent information regulator factor 2 related enzyme 1)/Foxo1(forkhead transcription factor FKHR) signaling pathway in myocardial tissues and H9c2 cells^[1].
Oxypaeoniflorin (0-30 μM) attenuates inflammatory effects via regulation of the toll-like receptor (TLR), extracellular signal-related kinase (ERK) and p38 mitogen-activated protein (MAP) kinases signaling pathways in LPS-stimulated RAW264.7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Oxypaeoniflorin (OPA; 10-40 mg/kg; intragastrical administration; every day; for 30 days) treatment significantly reduces disruption of cardiac function and improves the indicators of ejection fraction (EF) and fractional shortening (FS). Oxypaeoniflorin significantly reduces the release of myocardial infarction-related factors, such as the creatine kinase (CK-MB), cardiac troponin I (cTnI) and cardiac troponin T (cTnT)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 male mice (6-8 weeks of age, 20-25 g) bearing myocardial ischemia/reperfusion (MI/R) injury^[1]
Dosage:	10 mg/kg, 20 mg/kg, 40 mg/kg
Administration:	Intragastrical administration; every day; for 30 days
Result:	Significantly reduced disruption of cardiac function and improved the indicators of ejection fraction (EF) and fractional shortening (FS).

Molecular Weight

496.46

Formula

C₂₃H₂₈O₁₂

CAS No.

39011-91-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=CC=C(O)C=C1)OC[C@]([C@@]([C@]2(O)O3)([H])C4)([C@]3([H])O[C@]5(C2)C)[C@]45O[C@]([C@@H]([C@@H](O)[C@@H]6O)O)([H])O[C@@H]6CO

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (201.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0143 mL	10.0713 mL	20.1426 mL
5 mM	0.4029 mL	2.0143 mL	4.0285 mL
10 mM	0.2014 mL	1.0071 mL	2.0143 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.37%

Select Batch:

Data Sheet (282 KB) SDS (0 KB)

COA (259 KB) HNMR (302 KB) RP-HPLC (245 KB) MS (208 KB)

Handling Instructions (2659 KB)

References

[1]. Kai Wang, et al. Oxypaeoniflorin improves myocardial ischemia/reperfusion injury by activating the Sirt1/Foxo1 signaling pathway. Acta Biochim Pol. 2020 Jun 18;67(2):239-245. [Content Brief]

[2]. Feng C, et al. Pharmacokinetic properties of paeoniflorin, albiflorin and oxypaeoniflorin after oral gavage of extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba in rats. J Ethnopharmacol. 2010 Jul 20;130(2):407-13. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0143 mL	10.0713 mL	20.1426 mL	50.3565 mL
	5 mM	0.4029 mL	2.0143 mL	4.0285 mL	10.0713 mL
	10 mM	0.2014 mL	1.0071 mL	2.0143 mL	5.0357 mL
	15 mM	0.1343 mL	0.6714 mL	1.3428 mL	3.3571 mL
	20 mM	0.1007 mL	0.5036 mL	1.0071 mL	2.5178 mL
	25 mM	0.0806 mL	0.4029 mL	0.8057 mL	2.0143 mL
	30 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6786 mL
	40 mM	0.0504 mL	0.2518 mL	0.5036 mL	1.2589 mL
	50 mM	0.0403 mL	0.2014 mL	0.4029 mL	1.0071 mL
	60 mM	0.0336 mL	0.1679 mL	0.3357 mL	0.8393 mL
	80 mM	0.0252 mL	0.1259 mL	0.2518 mL	0.6295 mL
	100 mM	0.0201 mL	0.1007 mL	0.2014 mL	0.5036 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Oxypaeoniflorin39011-91-1OthersSirt1Foxo1monoterpeneglycosideCK-MBneuroprotectiveanti-inflammatoryanti-oxidantInhibitorinhibitorinhibit

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Oxypaeoniflorin

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