1. Neuronal Signaling
  2. Monoamine Oxidase
  3. TB5

TB5 

Cat. No.: HY-100975 Purity: ≥95.0%
Handling Instructions

TB5 is a potent, selective and reversible inhibitor of hMAO-B with a Ki value of 0.11±0.01 μM.

For research use only. We do not sell to patients.

TB5 Chemical Structure

TB5 Chemical Structure

CAS No. : 948841-07-4

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Solution
10 mM * 1 mL in DMSO USD 286 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 286 In-stock
Estimated Time of Arrival: December 31
Solid
50 mg USD 260 In-stock
Estimated Time of Arrival: December 31
100 mg USD 450 In-stock
Estimated Time of Arrival: December 31
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Description

TB5 is a potent, selective and reversible inhibitor of hMAO-B with a Ki value of 0.11±0.01 μM.

IC50 & Target

Ki: 0.11±0.01 μM (hMAO-B)[1]

In Vitro

TB5 and TB8 interacts with the catalytic site of hMAO-B and hMAO-A with a competitive mode of inhibition. TB5 shows the best inhibitory activity and higher selectivity toward hMAO-B, with Ki and SI values of 0.11±0.01μM and 13.18, respectively. hMAO-B inhibition by compound TB5 and hMAO-A inhibition by compound TB8 are completely reversed after 24 h of dialysis. Cytotoxicity studies show TB5 is nontoxic at 5 and 25 μM, resulting in cell viabilities of 95.75% and 84.59 %, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Compounds are dissolved in DMSO (5 mg/mL) and diluted with PBS/EtOH (70:30).Kinetic analyses are carried out for TB5 and TB8. A set of Lineweaver–Burk plots are constructed in the absence and presence of various concentrations of compounds TB5 and TC8. The set consists of five graphs, each constructed by measuring MAO-B and MAO-A catalytic rates at different substrate concentrations (0.1-1 μM). The first Lineweaver–Burk plot is constructed in the absence of inhibitor, while the remaining four graphs are constructed in the presence of different concentrations of TB5 and TB8[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

336.25

Formula

C₁₅H₁₄BrNOS

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 14.29 mg/mL (42.50 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9740 mL 14.8699 mL 29.7398 mL
5 mM 0.5948 mL 2.9740 mL 5.9480 mL
10 mM 0.2974 mL 1.4870 mL 2.9740 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

Compounds are dissolved in DMSO (5 mg/mL) and diluted with PBS/EtOH (70:30).Kinetic analyses are carried out for TB5 and TB8. A set of Lineweaver–Burk plots are constructed in the absence and presence of various concentrations of compounds TB5 and TC8. The set consists of five graphs, each constructed by measuring MAO-B and MAO-A catalytic rates at different substrate concentrations (0.1-1 μM). The first Lineweaver–Burk plot is constructed in the absence of inhibitor, while the remaining four graphs are constructed in the presence of different concentrations of TB5 and TB8[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

In vitro cytotoxicity of brominated thiophene chalcones and standard MAO inhibitors are tested in human HepG2 hepatic cancer cells at three different concentrations (1, 5 and 25 μM)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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TB5
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HY-100975
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