2. Neuronal Signaling PROTAC Epigenetics
  3. Monoamine Oxidase Ligands for Target Protein for PROTAC Histone Demethylase
  4. Tranylcypromine hydrochloride

Tranylcypromine hydrochloride  (Synonyms: SKF 385 hydrochloride)

Cat. No.: HY-17447A Purity: 99.91%
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Tranylcypromine hydrochloride (SKF 385 hydrochloride) is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Tranylcypromine hydrochloride inhibits LSD1, MAO A and MAO B with IC50s of 20.7, 2.3 and 0.95 μM, respectively. Tranylcypromine hydrochloride can be used for the research of depression.

For research use only. We do not sell to patients.

CAS No. : 1986-47-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
100 mg In-stock
250 mg In-stock
500 mg In-stock
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5 g   Get quote  

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of Tranylcypromine hydrochloride:

Tranylcypromine hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Tranylcypromine hydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Jun 23:117073.  [Abstract]

    Schematic diagram of Triflupromazine and Tranylcypromine hydrochloride alleviate primary cisplatin resistance in lung adenocarcinoma by promoting LDHA-mediated AMBRA1 ubiquitination. LDHA interacts with AMBRA1 via non-metabolic enzyme functions, promoting AMBRA1 ubiquitination and degradation, which leads to cisplatin resistance by regulating the cell cycle and apoptosis.

    Tranylcypromine hydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Jun 23:117073.  [Abstract]

    Tranylcypromine hydrochloride (0-100 μM) significantly inhibited LDHA expression and increased AMBRA1 protein levels in resistant cell line H1838.

    Tranylcypromine hydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Jun 23:117073.  [Abstract]

    CCK8 assay evaluating the survival of H1838 treated with serial concentrations of cisplatin in combination with Tranylcypromine hydrochloride (0-100 μM, 48 h).

    Tranylcypromine hydrochloride purchased from MedChemExpress. Usage Cited in: Biochem Pharmacol. 2025 Jun 23:117073.  [Abstract]

    Apoptosis analysis in H1838 cells treated with cisplatin accompanied by TRI (5 μM) and Tranylcypromine hydrochloride (50 μM) respectively for 48 h.

    View All Monoamine Oxidase Isoform Specific Products:

    View All Isoforms
    MAO-A MAO-B

    View All Histone Demethylase Isoform Specific Products:

    View All Isoforms
    KDM1/LSD1 KDM2 KDM3 KDM4 KDM5 KDM6 KDM7

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Tranylcypromine hydrochloride (SKF 385 hydrochloride) is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Tranylcypromine hydrochloride inhibits LSD1, MAO A and MAO B with IC50s of 20.7, 2.3 and 0.95 μM, respectively. Tranylcypromine hydrochloride can be used for the research of depression[1][2][3].

    IC50 & Target

    MAO-A

    2.3 μM (IC50)

    MAO-B

    0.95 μM (IC50)

    LSD1

     

    In Vitro

    Tranylcypromine hydrochlorid (50 μM-5 mM; 1 h or 12-14 h) inhibits histone and nucleosomal demethylation[1].
    Tranylcypromine hydrochlorid (2 μM; 3 h) shows a specific derepression of OCT4 transcription[1].
    Tranylcypromine hydrochlorid (0-100 μM; 15 min) shows IC50 values of 20.7, 2.3 and 0.95 μM for LSD1, MAO A and MAO B, respectively[2].
    Tranylcypromine hydrochlorid (0-800 μM) shows Ki values of 242.7, 101.9 and 16 μM for LSD1, MAO A and MAO B, respectively[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tranylcypromine hydrochloride Related Antibodies

    Western Blot Analysis[1]

    Cell Line: Sf21 insect cell line
    Concentration: 50 μM, 200 μM, 1 mM and 5 mM
    Incubation Time: 12-14 hours or 1 hour
    Result: Showed inhibitory activities of histone H3K4 demethylation and nucleosomal demethylation.

    RT-PCR[1]

    Cell Line: P19 EC cell line
    Concentration: 2 μM
    Incubation Time: 3 hours
    Result: Decreased Oct4 mRNA levels.
    In Vivo

    Tranylcypromine hydrochlorid (3 mg/kg; i.p. once daily for 3 days) decreases LPS-mediated microglial activation and proinflammatory cytokine COX-2 and IL-6 levels in wild-type mice[3].
    Tranylcypromine hydrochlorid (3 mg/kg; i.p. once daily for 7 days) down-regulates Aβ-mediate microglial activation in 5xFAD mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Wild-type mice[3]
    Dosage: 3 mg/kg
    Administration: Intraperitoneal injection; 3 mg/kg once daily for 3 days
    Result: Significantly down-regulated LPS-stimulated microglial activation in the cortex and Hippocampus, and LPS-induced astrocyte activation only in the cortex. Reduced LPS-induced COX-2 levels in hippocampus CA1, decreased LPS-evoked IL-6 levels in the cortex and hippocampus CA1 and suppressed LPS-mediated IL-1β levels in the cortex.
    Animal Model: 5xFAD mice[3]
    Dosage: 3 mg/kg
    Administration: Intraperitoneal injection; 3 mg/kg once daily for 7 days
    Result: Differentially regulated microglial and astrocyte activation in this mouse model of AD.
    Molecular Weight

    169.66

    Formula

    C9H12ClN
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    N[C@H]1[C@H](C2=CC=CC=C2)C1.[H]Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (589.41 mM; Need ultrasonic)

    DMSO : 100 mg/mL (589.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.8941 mL 29.4707 mL 58.9414 mL
    5 mM 1.1788 mL 5.8941 mL 11.7883 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    Concentration (final)

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.91%

    SDS (479 KB)

    COA (252 KB) HNMR (253 KB) RP-HPLC (237 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.8941 mL 29.4707 mL 58.9414 mL 147.3535 mL
    5 mM 1.1788 mL 5.8941 mL 11.7883 mL 29.4707 mL
    10 mM 0.5894 mL 2.9471 mL 5.8941 mL 14.7354 mL
    15 mM 0.3929 mL 1.9647 mL 3.9294 mL 9.8236 mL
    20 mM 0.2947 mL 1.4735 mL 2.9471 mL 7.3677 mL
    25 mM 0.2358 mL 1.1788 mL 2.3577 mL 5.8941 mL
    30 mM 0.1965 mL 0.9824 mL 1.9647 mL 4.9118 mL
    40 mM 0.1474 mL 0.7368 mL 1.4735 mL 3.6838 mL
    50 mM 0.1179 mL 0.5894 mL 1.1788 mL 2.9471 mL
    60 mM 0.0982 mL 0.4912 mL 0.9824 mL 2.4559 mL
    80 mM 0.0737 mL 0.3684 mL 0.7368 mL 1.8419 mL
    100 mM 0.0589 mL 0.2947 mL 0.5894 mL 1.4735 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Tranylcypromine hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tranylcypromine1986-47-6SKF 385SKF385SKF-385Monoamine OxidaseLigands for Target Protein for PROTACHistone DemethylaseMAOTarget Protein-binding Moietysmall molecularmethylationdemethylationP19 ECSf21neuroinflammationmicrogliaamyloid betaLPSInhibitorinhibitorinhibit

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