1. Neuronal Signaling
  2. Monoamine Oxidase
  3. Tranylcypromine hydrochloride

Tranylcypromine hydrochloride  (Synonyms: SKF 385 hydrochloride)

Cat. No.: HY-17447A Purity: 99.51%
COA Handling Instructions

Tranylcypromine hydrochloride (SKF 385 hydrochloride) is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Tranylcypromine hydrochloride inhibits LSD1, MAO A and MAO B with IC50s of 20.7, 2.3 and 0.95 μM, respectively. Tranylcypromine hydrochloride can be used for the research of depression.

For research use only. We do not sell to patients.

Tranylcypromine hydrochloride Chemical Structure

Tranylcypromine hydrochloride Chemical Structure

CAS No. : 1986-47-6

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Based on 4 publication(s) in Google Scholar

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Description

Tranylcypromine hydrochloride (SKF 385 hydrochloride) is an irreversible inhibitor of lysine-specific demethylase 1 (LSD1/BHC110) and monoamine oxidase (MAO). Tranylcypromine hydrochloride inhibits LSD1, MAO A and MAO B with IC50s of 20.7, 2.3 and 0.95 μM, respectively. Tranylcypromine hydrochloride can be used for the research of depression[1][2][3].

IC50 & Target

MAO-A

2.3 μM (IC50)

MAO-B

0.95 μM (IC50)

In Vitro

Tranylcypromine hydrochlorid (50 μM-5 mM; 1 h or 12-14 h) inhibits histone and nucleosomal demethylation[1].
Tranylcypromine hydrochlorid (2 μM; 3 h) shows a specific derepression of OCT4 transcription[1].
Tranylcypromine hydrochlorid (0-100 μM; 15 min) shows IC50 values of 20.7, 2.3 and 0.95 μM for LSD1, MAO A and MAO B, respectively[2].
Tranylcypromine hydrochlorid (0-800 μM) shows Ki values of 242.7, 101.9 and 16 μM for LSD1, MAO A and MAO B, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Sf21 insect cell line
Concentration: 50 μM, 200 μM, 1 mM and 5 mM
Incubation Time: 12-14 hours or 1 hour
Result: Showed inhibitory activities of histone H3K4 demethylation and nucleosomal demethylation.

RT-PCR[1]

Cell Line: P19 EC cell line
Concentration: 2 μM
Incubation Time: 3 hours
Result: Decreased Oct4 mRNA levels.
In Vivo

Tranylcypromine hydrochlorid (3 mg/kg; i.p. once daily for 3 days) decreases LPS-mediated microglial activation and proinflammatory cytokine COX-2 and IL-6 levels in wild-type mice[3].
Tranylcypromine hydrochlorid (3 mg/kg; i.p. once daily for 7 days) down-regulates Aβ-mediate microglial activation in 5xFAD mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type mice[3]
Dosage: 3 mg/kg
Administration: Intraperitoneal injection; 3 mg/kg once daily for 3 days
Result: Significantly down-regulated LPS-stimulated microglial activation in the cortex and Hippocampus, and LPS-induced astrocyte activation only in the cortex. Reduced LPS-induced COX-2 levels in hippocampus CA1, decreased LPS-evoked IL-6 levels in the cortex and hippocampus CA1 and suppressed LPS-mediated IL-1β levels in the cortex.
Animal Model: 5xFAD mice[3]
Dosage: 3 mg/kg
Administration: Intraperitoneal injection; 3 mg/kg once daily for 7 days
Result: Differentially regulated microglial and astrocyte activation in this mouse model of AD.
Molecular Weight

169.65

Formula

C9H12ClN

CAS No.
Appearance

Solid

Color

White to yellow

SMILES

N[C@H]1[C@H](C2=CC=CC=C2)C1.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (589.45 mM; Need ultrasonic)

DMSO : 100 mg/mL (589.45 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.8945 mL 29.4724 mL 58.9449 mL
5 mM 1.1789 mL 5.8945 mL 11.7890 mL
10 mM 0.5894 mL 2.9472 mL 5.8945 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (14.74 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.51%

References
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Tranylcypromine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Tranylcypromine hydrochloride
Cat. No.:
HY-17447A
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