Cat. No.: HY-N0133

Tangeretin

(Synonyms: Tangeritin; NSC53909; NSC618905)

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

Tangeretin (Tangeritin), a flavonoid from citrus fruit peels, has been proven to play an important role in anti-inflammatory responses and neuroprotective effects in several disease models, and is a Notch-1 inhibitor.

For research use only. We do not sell to patients.

Purity: 99.36%
CAS No.: 481-53-8
Formula: C₂₀H₂₀O₇
Molecular Weight:372.37

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Tangeretin Chemical Structure

CAS No.: 481-53-8

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

Notch-1

Cellular Effect

Cell Line	Type	Value	Description	References
3T3-L1	IC₅₀	48 μM Compound: 2, Tangeritin	Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation Anticorpulence activity against mouse 3T3L1 cells assessed as inhibition of lipid droplet accumulation	[PMID: 19054677]
A2780 ADR	IC₅₀	19 μM Compound: 18	Inhibition of P-gp expressed in A2780adr cells by calcein AM accumulation assay Inhibition of P-gp expressed in A2780adr cells by calcein AM accumulation assay	[PMID: 21354800]
HL-60	IC₅₀	>100 μM Compound: IX	Antiproliferative activity against HL60 after 24 hrs Antiproliferative activity against HL60 after 24 hrs	[PMID: 17391969]
HL-60	IC₅₀	10 ng/mL Compound: Tangeretin	Growth inhibition of human HL60 cells after 72 hrs by MTT assay Growth inhibition of human HL60 cells after 72 hrs by MTT assay	[PMID: 31398616]
HT-29	IC₅₀	77 μM Compound: 2, Tangeritin	Anticancer activity against human HT-29 cells after 72 hrs by MTT assay Anticancer activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 19054677]
KB	ED₅₀	15 μg/mL Compound: NSC-53909	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
MCF7	IC₅₀	17 μM Compound: 18	Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining	[PMID: 21354800]
MDCK	IC₅₀	19 μM Compound: 18	Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining	[PMID: 21354800]
MDCK	IC₅₀	39 μM Compound: 18	Inhibition of MDR1 expressed in MDCK cells using rhodamine 123 staining by flow cytometry Inhibition of MDR1 expressed in MDCK cells using rhodamine 123 staining by flow cytometry	[PMID: 21354800]
Monocyte	IC₅₀	30 μM Compound: Tangeretin	Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA Inhibition of TNFalpha expression in LPS-stimulated human monocytes treated 30 mins before LPS challenge measured after 14 hrs by ELISA	[PMID: 10096854]
RBL-2H3	IC₅₀	>500 μM Compound: 7	Antihistaminic activity in rat RBL2H3 cells assessed as inhibition of DNP-BSA-induced beta-hexosaminidase release preincubated for 10 mins before DNP-BSA challenge Antihistaminic activity in rat RBL2H3 cells assessed as inhibition of DNP-BSA-induced beta-hexosaminidase release preincubated for 10 mins before DNP-BSA challenge	[PMID: 14510616]
SH-SY5Y	IC₅₀	41.2 μM Compound: TAN, tangeretin	Growth inhibition of human SHSY5Y cells assessed as viable cells after 48 hrs by Trypan blue dye exclusion test Growth inhibition of human SHSY5Y cells assessed as viable cells after 48 hrs by Trypan blue dye exclusion test	[PMID: 18282757]

In Vitro

Tangeretin enhanced the radiosensitivity of GC cells as demonstrated by MTT and colony formation assays. Tangeretin also attenuated radiation-induced EMT, invasion and migration in GC cells, accompanied by a decrease in Notch-1, Jagged1/2, Hey-1 and Hes-1 expressions. Tangeretin triggered the upregulation of miR-410, a tumor-suppressive microRNA. Furthermore, re-expression of miR-410 prevented radiation-induced EMT and cell invasion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In this study, we investigated the in vivo anti-RSV activity of tangeretin in 3-week-old male BALB/c mice. A plaque reduction assay and fluorescence quantitative polymerase chain reaction (FQ-PCR) showed that tangeretin inhibited RSV replication in the lung of mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 481-53-8
Appearance Solid
Molecular Weight 372.37
Formula C₂₀H₂₀O₇
Color Off-white to yellow
SMILES

O=C1C=C(C2=CC=C(OC)C=C2)OC3=C(OC)C(OC)=C(OC)C(OC)=C13
Synonyms

Tangeritin; NSC53909; NSC618905
Structure Classification
- Flavonoids
- Flavones
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (15)

All Histological Imaging/StainingCell Proliferation/Viability AssayIHCWBCell Imaging/StainingIFRT-PCRELISA

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Journal Impact Factor

Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Phytomedicine

Nrf2-activated mitophagy and ferroptosis suppression synergistically mediate tangeretin's protection against hepatic ischemia-reperfusion injury. [Abstract]

2025 Sep:145:157034. PMID: 40617054

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:157034. [Abstract]

Representative image of H&E-stained liver sections, scale bar: 200 μm. I/R-induced centrilobular necrosis was indicated by the dotted line. The results indicated a significant decrease in hepatic necrotic areas in Tangeretin (TAN, 10-40 mg/kg; i.p.; single dose) treated mice compared to the control group receiving nothing.

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:157034. [Abstract]

Representative pictures of liver stained with Ly-6 G (neutrophils) and F4/80 (macrophages), scale bar, 100 μm. Immunohistochemical analysis revealed significant decrease in hepatic inflammation in Tangeretin (TAN, 10-40 mg/kg; i.p.; single dose)-treated mice, and the infiltration of Ly6G⁺ neutrophils and F4/80⁺ macrophages was significantly decreased in TAN-treated mice livers compared to untreated mice.

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep:145:157034. [Abstract]

Hepatocytes were treated with or without the treatment of Tangeretin (TAN) (25 μM) in the presence or absence of OGD/R-treatment for 6 h. At the end of experiment, Mitotracker (Green) and Lysotracker (Red) were co-stained, scale bars: 20 μm. The results showed that TAN treatment led to an enhanced colocalization of the mitochondrial probe Mitotracker with the lysosomal probe Lysotracker.
Phytomedicine

Bruceine D suppresses CAF-promoted TNBC metastasis under TNF-α stimulation by inhibiting Notch1-Jagged1/NF-κB(p65) signaling. [Abstract]

2024 Jan:123:154928. PMID: 38043386
Phytomedicine

Tangeretin suppresses osteoarthritis progression via the Nrf2/NF-κB and MAPK/NF-κB signaling pathways. [Abstract]

2022 Apr:98:153928. PMID: 35104760

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Apr:98:153928. [Abstract]

Results of CCK-8 assay showing the effects of different Tangeretin concentrations (0, 5, 10, 20, 40, and 80 μM for 24 h) on the viability of chondrocytes.

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Apr:98:153928. [Abstract]

Chondrocytes were treated with IL-1β (10 ng/ml) in the presence or absence of Tangeretin (5, 10, 20 μM) for 24 h. The protective effects of Tangeretin were examined by quantifying expression of collagen II, aggrecan, ADAMTS5, and MMP-13.

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Apr:98:153928. [Abstract]

Immunofluorescence staining analysis of MMP13 and collagen II in chondrocytes treated with IL-1β (10 ng/ml) in the presence or absence Tangeretin (20 μM) for 24 h. Scale bar: 30 μm.

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Apr:98:153928. [Abstract]

The chondrocytes were seeded in the culture dish for 24 h and then exposed to IL-1β (10 ng/ml) with or without Tangeretin (5, 10, 20 μM) for additional 24 h. Real-time PCR for the effects of Tangeretin on mRNAs levels of IL-6, TNF-α, iNOS and COX-2 in chondrocytes. The expression levels of these genes were normalized to the expression of β-actin.

Tangeretin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2022 Apr:98:153928. [Abstract]

The chondrocytes were seeded in the culture dish for 24 h and then exposed to IL-1β (10 ng/ml) with or without Tangeretin (5, 10, 20 μM) for additional 24 h. ELISA or Griess reagent test for the effects of Tangeretin on PGE2, IL-6, TNF-α, and NO levels in the cell culture supernatants after indicated treatment.
Biomed Pharmacother

Developing neobavaisoflavone nanoemulsion suppresses lung cancer progression by regulating tumor microenvironment. [Abstract]

2020 Sep;129:110369. PMID: 32563983
Ind Crops Prod

2025 Dec 11;239:122458.
J Agric Food Chem

Tangeretin Protects Mice from Alcohol-Induced Fatty Liver by Activating Mitophagy through the AMPK-ULK1 Pathway. [Abstract]

2022 Sep 14;70(36):11236-11244. PMID: 36063077
J Agric Food Chem

Tangeretin Inhibits BACE1 Activity and Attenuates Cognitive Impairments in AD Model Mice. [Abstract]

2022 Feb 9;70(5):1536-1546. PMID: 35084179
Eur J Pharmacol

Bruceine D inhibits CAF-promoted angiogenesis of breast cancer via suppressing IL-6-mediated activation of the STAT3/Notch1/VEGFR2 axis. [Abstract]

2025 Sep 15:1003:177994. PMID: 40714073
Microb Pathogenesis

Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice. [Abstract]

2020 Jan;138:103826. PMID: 31676364
Am J Cancer Res

PRDM16 from hepatic stellate cells-derived extracellular vesicles promotes hepatocellular carcinoma progression. [Abstract]

2023 Nov 15;13(11):5254-5270. PMID: 38058806
Biochem Biophys Res Commun

Tangeretin protects mice from diet-induced metabolic inflammation via activating adipose lactate accumulation and macrophage M2 polarization. [Abstract]

2022 Nov 19:630:16-23. PMID: 36126465
Rev Bras Farmacogn

26 July 2022.
Research Square Preprint

2021 Oct.

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (67.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6855 mL

13.4275 mL

26.8550 mL

5 mM

0.5371 mL

2.6855 mL

5.3710 mL

10 mM

0.2686 mL

1.3428 mL

2.6855 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 1 mg/mL (2.69 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay

The effect of tangeretin on RAW264.7 cells was determined using a MTT assay as previously reported.(13) Briefly, RAW264.7 cells (1 × 104 cells/well) were seeded in a 96-well plate for 24 h and treated with different concentrations of tangeretin (6.3–50.0 μM) and dimethyl sulfoxide (DMSO) (vehicle control, 0.01 and 0.1%) for 10 or 48 h. The absorbance was measured at 570 nm using an enzyme immunoassay (EIA) reader (Thermo Scientific, Waltham, MA), and cell viability (%) was calculated as follows: [(absorbance of the test group – absorbance of the blank control)/(absorbance of the control group – absorbance of the blank control)] × 100.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Animal administration [2] The mice were maintained in an air-conditioned, pathogen-free room (temperature of 24 ± 2 °C, with a 12 h light/dark cycle from 6:00 am to 6:00 pm) with free access to food and water. Mice were randomly divided into five groups (n = 10) as follows: normal (control), RSV-challenged, and three treatment groups administered 25, 50, or 100 mg/kg/day tangeretin dissolved in saline. The control and RSV-challenged groups received equal volumes of saline. During the experiment, mice in the treatment groups were intragastrically administrated tangeretin for 3 days consecutively before RSV stimulation. Mice were lightly anesthetized with diethyl ether and intranasally challenged with RSV Long strain [6.7 × 106 plaque-forming units (PFU)] on day 4 after tangeretin treatment, while the control group was sham-infected with an equal volume of HEp-2 cell lysate, which was centrifuged under the same conditions as the viral suspensions. The mice were weighed during the experiment and sacrificed on day 5 post-infection after anesthetizing them with chloral hydrate (Figure 1B). The lung tissues were removed and weighed, and the lung index was calculated using the following formula: lung index = lung weight/body weight.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Portuguese - PT (481 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang X, et al. Tangeretin enhances radiosensitivity and inhibits the radiation-induced epithelial-mesenchymal transition of gastric cancer cells. Oncol Rep. 2015 Jul;34(1):302-10. [Content Brief]

[2]. Xu JJ, et al. Tangeretin from Citrus reticulate Inhibits Respiratory Syncytial Virus Replication and Associated Inflammation in Vivo. J Agric Food Chem. 2015 Nov 4;63(43):9520-7. [Content Brief]

[3]. Hagenlocher Y, et al. Citrus peel polymethoxyflavones nobiletin and tangeretin suppress LPS- and IgE-mediated activation of human intestinal mast cells. Eur J Nutr. 2017 Jun;56(4):1609-1620. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6855 mL	13.4275 mL	26.8550 mL	67.1375 mL
	5 mM	0.5371 mL	2.6855 mL	5.3710 mL	13.4275 mL
	10 mM	0.2686 mL	1.3428 mL	2.6855 mL	6.7138 mL
	15 mM	0.1790 mL	0.8952 mL	1.7903 mL	4.4758 mL
	20 mM	0.1343 mL	0.6714 mL	1.3428 mL	3.3569 mL
	25 mM	0.1074 mL	0.5371 mL	1.0742 mL	2.6855 mL
	30 mM	0.0895 mL	0.4476 mL	0.8952 mL	2.2379 mL
	40 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6784 mL
	50 mM	0.0537 mL	0.2686 mL	0.5371 mL	1.3428 mL
	60 mM	0.0448 mL	0.2238 mL	0.4476 mL	1.1190 mL

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Tangeretin

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Publications Citing Use of MedChemExpress (MCE) Tangeretin

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tangeretin Related Classifications

Cat. No.: HY-N0133