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VI 16832 

Cat. No.: HY-18623 Purity: 98.05%
Handling Instructions

VI 16832 is a broad spectrum Type I kinase inhibitor which can be used as an enrichment tool for the comparative expression analysis of protein kinases in different cancer cell lines.

For research use only. We do not sell to patients.

VI 16832 Chemical Structure

VI 16832 Chemical Structure

CAS No. : 1430218-51-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 341 In-stock
Estimated Time of Arrival: December 31
1 mg USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 396 In-stock
Estimated Time of Arrival: December 31
10 mg USD 600 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1800 In-stock
Estimated Time of Arrival: December 31
100 mg USD 2520 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

VI 16832 is a broad spectrum Type I kinase inhibitor which can be used as an enrichment tool for the comparative expression analysis of protein kinases in different cancer cell lines.

IC50 & Target

Type I kinase[1]

In Vitro

VI 16832 is a broad spectrum Type I kinase inhibitor[1]. Phosphoproteomics analysis of VI 16832-enriched fractions from MV4-11, HCT116, or 435S cells results in more than 8500 phosphopeptide identifications. These translate into almost 1700 distinct phosphopeptide species derived from 212 different members of the protein kinase superfamily. Analysis of VI 16832-retained proteins from the three cancer cell lines considerably increases the overall number of identified phosphorylation sites on protein kinases. Considering the sum of all phosphopeptide intensities as a measure for VI 16832-enriched protein amount, more than 80% is derived from protein kinases[2].

Solvent & Solubility
In Vitro: 

DMSO : 83.3 mg/mL (212.79 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5545 mL 12.7724 mL 25.5447 mL
5 mM 0.5109 mL 2.5545 mL 5.1089 mL
10 mM 0.2554 mL 1.2772 mL 2.5545 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[2]

Cells are lysed in a volume of 35 to 40 mL per experiment. The protein amounts of the starting extracts used in the first and second experiments are: 435S, 85 and 120 mg; HCT116, 240 and 175 mg; MV4-11, 180 and 120 mg. Lysates are adjusted to 1 M NaCl prior to loading onto the VI 16832 column at a flow rate of 0.07 mL/min. Subsequent washing and elution steps are performed. Protein-containing elution fractions are lyophilized, resuspended in one tenth of the initial volume, and then desalted by protein precipitation prior to gel electrophoresis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

391.47

Formula

C₂₂H₂₅N₅O₂

CAS No.

1430218-51-1

SMILES

O=C1C=CC2=CN=C(NC3=CC=C(OCCN)C=C3)N=C2N1C4C(C5)CCC5C4

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
VI 16832
Cat. No.:
HY-18623
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VI 16832

Cat. No.: HY-18623