1. PI3K/Akt/mTOR
  2. Akt
  3. A-674563


Cat. No.: HY-13254 Purity: 99.87%
Handling Instructions

A-674563 is an orally active and selective Akt1 inhibitor with a Ki of 11 nM.

For research use only. We do not sell to patients.

A-674563 Chemical Structure

A-674563 Chemical Structure

CAS No. : 552325-73-2

Size Price Stock
5 mg USD 250 Ask For Quote & Lead Time
10 mg USD 450 Ask For Quote & Lead Time
25 mg USD 850 Ask For Quote & Lead Time
50 mg USD 1350 Ask For Quote & Lead Time
100 mg USD 1950 Ask For Quote & Lead Time

* Please select Quantity before adding items.

Other In-stock Forms of A-674563:

Other Forms of A-674563:

Top Publications Citing Use of Products

    A-674563 purchased from MCE. Usage Cited in: Oncotarget. 2016 May 17;7(20):29131-42.

    A674563 selectively inhibits FLT3-ITD. Inhibitory Effects of A674563 against auto-phosphorylation of FLT3 wt/mt kinases in the FLT3 wt/mt transformed BaF3 isogenic cell lines.

    View All Akt Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review


    A-674563 is an orally active and selective Akt1 inhibitor with a Ki of 11 nM.

    IC50 & Target


    11 nM (Ki)


    16 nM (Ki)


    46 nM (Ki)


    110 nM (Ki)


    260 nM (Ki)


    360 nM (Ki)


    580 nM (Ki)


    1.1 μM (Ki)


    1.2 μM (Ki)


    2.6 μM (Ki)


    5.4 μM (Ki)


    13 μM (Ki)

    In Vitro

    A-674563 slows proliferation of tumor cells with an EC50 of 0.4 μM[1].
    A563 (0-10 µM) significantly decreases GSK3 and MDM2 phosphorylation in STS cells. A563 shows inhibitory effect on all STS cell lines, with IC50 values at 48 hours ranging from 0.22 µM (SW684) to 0.35 µM (SKLMS1). A563 induces G2 cell cycle arrest and apoptosis in STS cells. A563 (1 µM/12 hr) upregulates the expression of GADD45A independent of p53[2].
    A-674563 (10-1000 nM) is anti-proliferative and cytotoxic in cultured human melanoma cells, induces melanoma cell apoptotic death, inhibited by caspase inhibitors, and inhibits melanoma cells via Akt-dependent and -independent mechanisms[3].
    A-674563 is cytotoxic and anti-proliferative when added to U937 and AmL progenitor cells, activates caspase-3/9 and apoptosis in U937 and AmL progenitor cells, and manipulates other signalings in AmL cells whiling blocking Akt[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    A-674563 (40 mg/kg/d, p.o.) shows no significant monotherapy activity, but the efficacy of the combination therapy (A-674563+paclitaxel) is significantly improved in the PC-3 prostate cancer xenograft model. A-674563 (20, 100 mg/kg) increases plasma insulin in an oral glucose tolerance test[1].
    A563 (20 mg/kg/bid; p.o.) exhibits slow tumor growth and a significant difference in tumor volume without significant weight loss of mice. A563-treated tumors express increased levels of GADD45α and decreased levels of PCNA (a nuclear marker for proliferation). Additionally, TUNEL assay staining levels (marker for apoptosis) increase in the A563-treated specimens[2].
    A-674563 (25, 100 mg/kg, lavage daily) potently inhibits A375 xenograft growth in mice[3].
    A-674563 (15, 40 mg/kg) injection inhibits U937 xenograft in vivo growth, and improves mice survival[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.



    N[[email protected]@H](CC1=CC=CC=C1)COC2=CC(C3=CC4=C(C=C3)NN=C4C)=CN=C2


    Room temperature in continental US; may vary elsewhere.


    4°C, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 83.33 mg/mL (232.48 mM; Need ultrasonic)

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7899 mL 13.9493 mL 27.8987 mL
    5 mM 0.5580 mL 2.7899 mL 5.5797 mL
    10 mM 0.2790 mL 1.3949 mL 2.7899 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (5.80 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 5.75 mg/mL (16.04 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are provided by MCE.
    Cell Assay

    The cells on 96-well plates are gently washed with 200 μL of PBS. Alamar Blue reagent is diluted 1:10 in normal growth media. The diluted Alamar Blue reagent (100 μL) is added to each well on the 96-well plates and incubated until the reaction is complete. Analysis is done using an fmax Fluorescence Microplate Reader, set at the excitation wavelength of 544 nm and emission wavelength of 595 nm. Data are analyzed using SOFTmax PRO software.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration

    Immunocompromised male scid mice are at 6 to 8 weeks of age. The 1×106 3T3-Akt1 or 2×106 MiaPaCa-2 and PC-3 cells in 50% Matrigel are inoculated s.c. into the flank. For early treatment studies, mice are randomLy assigned to treatment groups and therapy is initiated the day after inoculation. Ten animals are assigned to each group, including controls. For established tumor studies, tumors are allowed to reach a designated size and mice are assigned to treatment groups of equal tumor size (n=10 mice per group). Tumor size is evaluated by twice weekly measurements with digital calipers. Tumor volume is estimated using the formula: V=L×W2/2. A-443654 is given s.c. in a vehicle of 0.2% HPMC. A-674563 is given orally in a vehicle of 5% dextrose. Gemcitabine and paclitaxel are added to the assay.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.


    Purity: 99.87%

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2


    A-674563A674563A 674563AktPKBProtein kinase BInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name



    Applicant Name *


    Email address *

    Phone number *


    Organization name *

    Department *


    Requested quantity *

    Country or Region *



    Bulk Inquiry

    Inquiry Information

    Product Name:
    Cat. No.:
    MCE Japan Authorized Agent: