Green DND-26
Based on 3 publication(s) in Google Scholar
Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.77%
- CAS. Nr.: 220524-71-0
- Formel: C18H25BF2N4O
- Molecular Weight:362.23
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Speicherung:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Green DND-26
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Biologische Aktivität
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of Stock Solution and Working Solution
1.1 Prepare a 1 mM stock solution using anhydrous DMSO.
1.2 Dilute the stock solution with preheated serum-free cell culture medium or PBS to prepare a 50-100 nM working solution.
Note: Please adjust the concentration of the dye working solution according to the actual situation, and prepare it fresh before use.
2. Cell Staining
2.1 Suspension Cells: Centrifuge at 1000 g for 3-5 minutes at 4°C, discard the supernatant. Wash twice with PBS, 5 minutes each time.
Adherent cells: Discard the cell culture medium, wash twice with PBS, 5 minutes each time.
2.2 Add Green DND-26 working solution and incubate at room temperature in the dark for 30 minutes.
2.3 Discard the staining solution, wash twice with PBS, 5 minutes each time.
2.5 Resuspend the cells in serum-free cell culture medium or PBS, and analyze using a fluorescence microscope or flow cytometry.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS. Nr. 220524-71-0
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Appearance Solid
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Molecular Weight 362.23
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Formel C18H25BF2N4O
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Color Orange to red
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SMILES
O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])NCCN(C)C
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (3)
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Journal Impact Factor
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Most Recent
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ACS Nano
Long-Term Monitoring of Enzymatic Activity at the Single-Organelle Level via Reversed Plasmonic Resonance Energy Transfer. [Abstract]2025 Sep 30. PMID: 41025602 -
RSC Adv
One-pot synthesis of fluorescent cap1 mRNAs using labelled trinucleotide m7G-cap analogs for real-time in vitro and in vivo mRNA tracking. [Abstract]2026 May 14;16(28):25548-25556. PMID: 42158732 -
Cancer Chemother Pharmacol
Osthole inhibits malignant phenotypes and induces ferroptosis in KRAS-mutant colorectal cancer cells via suppressing AMPK/Akt signaling. [Abstract]2023 Aug;92(2):119-134. PMID: 37318525
Lösungsmittel & Löslichkeit
DMSO : 125 mg/mL (345.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Sun B, et al. Acid-Activatable Transmorphic Peptide-Based Nanomaterials for Photodynamic Therapy. Angew Chem Int Ed Engl. 2020;59(46):20582-20588. [Content Brief]
[2]. Van der Velden JL, et al. LysoTracker is a marker of differentiated alveolar type II cells. Respir Res. 2013;14(1):123. Published 2013 Nov 11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7607 mL | 13.8034 mL | 27.6068 mL | 69.0169 mL |
| 5 mM | 0.5521 mL | 2.7607 mL | 5.5214 mL | 13.8034 mL | |
| 10 mM | 0.2761 mL | 1.3803 mL | 2.7607 mL | 6.9017 mL | |
| 15 mM | 0.1840 mL | 0.9202 mL | 1.8405 mL | 4.6011 mL | |
| 20 mM | 0.1380 mL | 0.6902 mL | 1.3803 mL | 3.4508 mL | |
| 25 mM | 0.1104 mL | 0.5521 mL | 1.1043 mL | 2.7607 mL | |
| 30 mM | 0.0920 mL | 0.4601 mL | 0.9202 mL | 2.3006 mL | |
| 40 mM | 0.0690 mL | 0.3451 mL | 0.6902 mL | 1.7254 mL | |
| 50 mM | 0.0552 mL | 0.2761 mL | 0.5521 mL | 1.3803 mL | |
| 60 mM | 0.0460 mL | 0.2301 mL | 0.4601 mL | 1.1503 mL | |
| 80 mM | 0.0345 mL | 0.1725 mL | 0.3451 mL | 0.8627 mL | |
| 100 mM | 0.0276 mL | 0.1380 mL | 0.2761 mL | 0.6902 mL |
Green DND-26 Related Classifications
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