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Cat. No.: HY-109080A

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Belvarafenib TFA

(Synonyms: HM95573 TFA; GDC-5573 TFA; RG6185 TFA)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Belvarafenib TFA (HM95573 TFA) is a potent and pan RAF (Rapidly Accelerated Fibrosarcoma) inhibitor, with IC₅₀s of 56 nM, 7 nM and 5 nM for B-RAF, B-RAFv^600E and C-RAF respectively.

For research use only. We do not sell to patients.

CAS No.: 2443966-84-3
Formula: C₂₅H₁₇ClF₄N₆O₃S
Molecular Weight:592.95

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Belvarafenib TFA Chemical Structure

CAS No.: 2443966-84-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (6)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Belvarafenib TFA (HM95573 TFA) is a potent and pan RAF (Rapidly Accelerated Fibrosarcoma) inhibitor, with IC₅₀s of 56 nM, 7 nM and 5 nM for B-RAF, B-RAFv^600E and C-RAF respectively^[1].

IC₅₀ & Target

IC50: 56 nM(B-RAF), 7 nM(B-RAFv^600E), 5 nM(C-RAF)^[1].

All Raf Isoforms :

In Vitro

Belvarafenib (Example 116) is a potent and pan RAF inhibitor with antineoplastic activity. The IC₅₀ values of Belvarafenib are 56 nM, 7 nM and 5 nM for B-RAF, B-RAFv^600E and C-RAF respectively. It also shows high inhibitory activity for FMS, DDR1 and DDR2 kinases, with IC₅₀s of 10 nM, 23 nM and 44 nM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2443966-84-3
Appearance Solid
Molecular Weight 592.95
Formula C₂₅H₁₇ClF₄N₆O₃S
Color White to off-white
SMILES

O=C(C(C1=NC=N2)=CSC1=C2N)NC(C3=C4C(NC(C=CC=C5Cl)=C5F)=NC=C3)=C(C=C4)C.OC(C(F)(F)F)=O
Synonyms

HM95573 TFA; GDC-5573 TFA; RG6185 TFA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (6)

All RT-PCRWBApoptosis AnalysisIFIn Vivo Efficacy Study

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Journal Impact Factor

Cell Death Dis

mTOR inhibition enhances the antitumor efficacy of pan-RAF-MEK blockade by inhibiting the ATF4-MTHFD2 pathway. [Abstract]

2026 May 6;17(1):600. PMID: 42091854

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Fold change of MTHFD2 transcripts following indicated treatment in WM3406 cells (left) and MeWo cells (right) treated with INK128, Cobimetinib and Belvarafenib (25 nM).

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Western blot analysis of the indicated proteins in WM3406 (left) and MeWo (right) cells with ATF4 knockdown or Belvarafenib (25 nM) plus Cobimetinib treatment.

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Percent apoptosis detected in DMSO- or Belvarafenib plus Cobimetinib- or Belvarafenib (25 nM) plus Cobimetinib plus nucleosides-treated WM3406 or MeWo cells.

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Representative images of WM3406 and MeWo cells following Belvarafenib plus Cobimetinib- or Belvarafenib (25 nM) plus Cobimetinib plus nucleosides-treated for 18 h and processed for γH2AX (green) immunofluorescence.

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Tumor growth curve comparing MaNRAS1007 melanomas grown on C57BL/6N host mice with or without Belvarafenib (5 mg/kg, i.g.) plus Cobimetinib treatments.
Proc Natl Acad Sci U S A

Oncogenic dependency plays a dominant role in the immune response to cancer. [Abstract]

2023 Oct 10;120(41):e2308635120. PMID: 37782788
J Invest Dermatol

Combined Inhibition of MNK Signaling and BET Proteins Reveals TGM2 as a Novel Vulnerability in Melanoma. [Abstract]

2025 Apr;145(4):979-984.e5. PMID: 39357785
Res Sq

2025 Jul 18.
Patent

US20240239801A1.
Patent

US20240140945A1.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. WO 2013/100632 A1.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Belvarafenib TFA

6 Cited Publications

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (6)
Purity & Documentation
References

1/16
1/16

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Customer Validation
Fold change of MTHFD2 transcripts following indicated treatment in WM3406 cells (left) and MeWo cells (right) treated with INK128, Cobimetinib and Belvarafenib (25 nM).
1/16

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Customer Validation
Western blot analysis of the indicated proteins in WM3406 (left) and MeWo (right) cells with ATF4 knockdown or Belvarafenib (25 nM) plus Cobimetinib treatment.
1/16

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Customer Validation
Percent apoptosis detected in DMSO- or Belvarafenib plus Cobimetinib- or Belvarafenib (25 nM) plus Cobimetinib plus nucleosides-treated WM3406 or MeWo cells.
1/16

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Customer Validation
Representative images of WM3406 and MeWo cells following Belvarafenib plus Cobimetinib- or Belvarafenib (25 nM) plus Cobimetinib plus nucleosides-treated for 18 h and processed for γH2AX (green) immunofluorescence.
1/16

Belvarafenib TFA purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 May 6;17(1):600. [Abstract]

Customer Validation
Tumor growth curve comparing MaNRAS1007 melanomas grown on C57BL/6N host mice with or without Belvarafenib (5 mg/kg, i.g.) plus Cobimetinib treatments.

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Belvarafenib TFA

Publications Citing Use of MedChemExpress (MCE) Belvarafenib TFA

All Raf Isoforms

Biological Activity

Chemical Information

Publications (6)

Purity & Documentation

References

Calculators

Help & FAQs

Belvarafenib TFA Related Classifications

HY-109080A Related Classifications