6-Aminonicotinamide
Based on 22 publication(s) in Google Scholar
6-Aminonicotinamide, a potent antimetabolite of nicotinamide, is competitive NADP+-dependent enzyme glucose-6-phosphate dehydrogenase (G6PD) inhibitor (Ki=0.46 μM). 6-Aminonicotinamide resultis ATP depletion and synergizes with DNA-crosslinking chemotherapy agents, such as Cisplatin (HY-17394), in killing cancer cells.
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- Pureté: 99.95%
- CAS No.: 329-89-5
- Formule: C6H7N3O
- Masse moléculaire:137.14
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Stockage:
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
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Publications Citing Use of MedChemExpress (MCE) 6-Aminonicotinamide
More- Immunity. 2026 Feb 24:S1074-7613(26)00030-0. [Abstract]
- Nat Biomed Eng. 2025 Sep;9(9):1547-1556. [Abstract]
- Redox Biol. 2024 Aug:74:103236. [Abstract]
- Cell Death Differ. 2026 May 12. [Abstract]
- Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]
- Cancer Lett. 2026 May 1:645:218384. [Abstract]
- Phytomedicine. 2024 Jul:129:155657. [Abstract]
- Cell Rep. 2026 Apr 25;45(5):117297. [Abstract]
- Int J Mol Sci. 2023 Sep 30;24(19):14786. [Abstract]
- J Nutr Biochem. 2023 Dec:122:109452. [Abstract]
- PLoS Pathog. 2026 Apr 22;22(4):e1014165. [Abstract]
- PLoS Pathog. 2026 Jan 27;22(1):e1013913. [Abstract]
- J Cereb Blood Flow Metab. 2025 Sep 25:271678X251377678. [Abstract]
- Metabolites. 2022 Dec 7;12(12):1227. [Abstract]
- J Biol Chem. 2026 Apr;302(4):111345. [Abstract]
- Eur J Immunol. 2025 Dec;55(12):e70087. [Abstract]
- Eur J Immunol. 2025 Oct;55(10):e70068. [Abstract]
- J Photochem Photobiol B. 2026 Jun:279:113441. [Abstract]
- bioRxiv. 2026 Apr 4:2026.04.03.716381. [Abstract]
- bioRxiv. 2025 Oct 14.
- University of Kentucky. 2025.
- Research Square Print. 2023 Feb 14.
Customer Validation & Images
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In Vivo Efficacy Study
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IF
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Flow Cytometry
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Cell Proliferation/Viability Assay
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ELISA
Activité biologique
6-Aminonicotinamide (100 nM; 7 days) causes a significant decrease in the human AR+, hormone-sensitive prostate cancer cell lines LNCaP and LAPC4, as well as the CRPC-derivative C4-2 and 22Rv1 cell models. 6-Aminonicotinamide (100 nM ± 10 nM R1881 as indicated for 3 days) increases both basal- and R1881-mediated ROS levels, suggesting 6-Aminonicotinamide is blocking the cells' antioxidant defense. 6-Aminonicotinamide also increases ROS levels in C4-2 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 329-89-5
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Appearance Solid
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Masse moléculaire 137.14
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Formule C6H7N3O
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Color White to off-white
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SMILES
NC1=CC=C(C(N)=O)C=N1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
Publications (22)
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Journal Impact Factor
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Most Recent
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Immunity
Polystyrene microplastic-induced pathophysiology is driven by disruption of efferocytosis. [Abstract]2026 Feb 24:S1074-7613(26)00030-0. PMID: 41742421 -
Nat Biomed Eng
A live bacteria enzyme assay for identification of human disease mutations and drug screening. [Abstract]2025 Sep;9(9):1547-1556. PMID: 40307426
6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Nat Biomed Eng. 2025 Sep;9(9):1547-1556. [Abstract]
Growth rates of humanized E. coli in LEICA with different compounds. 6-Aminonicotinamide (6AN) (100 μM) completely halted the growth of humanized E. coli for G6PD in LEICA.
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Redox Biol
Glucose-6-phosphate dehydrogenase alleviates epileptic seizures by repressing reactive oxygen species production to promote signal transducer and activator of transcription 1-mediated N-methyl-d-aspartic acid receptors inhibition. [Abstract]2024 Aug:74:103236. PMID: 38875958 -
Cell Death Differ
A neutrophil-intrinsic CKLF1-PKM2 axis drives glycolytic flux for de novo DAG synthesis and pro-inflammatory ROS production. [Abstract]2026 May 12. PMID: 42120940 -
Cell Death Dis
Post-irradiation dietary restriction impairs hematopoiesis via inhibition of the pentose phosphate pathway in hematopoietic stem and progenitor cells. [Abstract]2026 Jan 7;17(1):8. PMID: 41500989
6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]
Experimental scheme. Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays and fed with an AL diet. Mice were then injected intraperitoneally with 10 mg/kg of 6-Aminonicotinamide (6-AN) or saline daily for 7 days post-irradiation, and were continued on an AL diet until 1 month post-irradiation. G6PD enzyme activity (B) and NADPH content (C) in BM cells of AL mice 1 month post-irradiation, analyzed using ELISA.
6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]
Representative images of γH2AX and 53BP1 colocalization in BM cells of AL mice 1 month post-irradiation, determined by immunofluorescent staining. Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays and fed with an AL diet. Mice were then injected intraperitoneally with 10 mg/kg of 6-Aminonicotinamide (6-AN) or saline daily for 7 days post-irradiation, and were continued on an AL diet until 1 month post-irradiation.
6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2026 Jan 7;17(1):8. [Abstract]
Representative FACS Plots of HSCs (Left) and CMPs/GMPs/MEPs (Right) of AL mice 1 month post-irradiation. .Wild-type C57BL/6 mice (2 months old) were irradiated with 4.5 Gy X-rays and fed with an AL diet. Mice were then injected intraperitoneally with 10 mg/kg of 6-Aminonicotinamide (6-AN) or saline daily for 7 days post-irradiation, and were continued on an AL diet until 1 month post-irradiation.
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Cancer Lett
GPI inactivation mediates pentose phosphate pathway flux switch-on inducing temozolomide resistance in glioma stem cell. [Abstract]2026 May 1:645:218384. PMID: 41763452 -
Phytomedicine
Gambogic acid exhibits promising anticancer activity by inhibiting the pentose phosphate pathway in lung cancer mouse model. [Abstract]2024 Jul:129:155657. PMID: 38692076 -
Cell Rep
SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization. [Abstract]2026 Apr 25;45(5):117297. PMID: 42035418 -
Int J Mol Sci
PIP5Kγ Mediates PI(4,5)P2/Merlin/LATS1 Signaling Activation and Interplays with Hsc70 in Hippo-YAP Pathway Regulation. [Abstract]2023 Sep 30;24(19):14786. PMID: 37834234 -
J Nutr Biochem
F6P/G6P-mediated ChREBP activation promotes the insulin resistance-driven hepatic lipid deposition in zebrafish. [Abstract]2023 Dec:122:109452. PMID: 37748621 -
PLoS Pathog
Nicotinamide metabolism is essential for Hepatitis C Virus replication and the production of infectious Lipo-Viro-Particles. [Abstract]2026 Apr 22;22(4):e1014165. PMID: 42018568 -
PLoS Pathog
White spot syndrome virus IE1 protein hijacks the host pentose phosphate pathway to fuel viral replication. [Abstract]2026 Jan 27;22(1):e1013913. PMID: 41592137 -
J Cereb Blood Flow Metab
G6PD deficiency is implicated in Circle of Willis variants in stroke patients and impairs brain vasculature in zebrafish. [Abstract]2025 Sep 25:271678X251377678. PMID: 40995861 -
Metabolites
Lipidomics and Transcriptomics Differ Liposarcoma Differentiation Characteristics That Can Be Altered by Pentose Phosphate Pathway Intervention. [Abstract]2022 Dec 7;12(12):1227. PMID: 36557266
6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Metabolites. 2022 Dec 7;12(12):1227. [Abstract]
6-Aminonicotinamide (6-AN; 1 μM; 0-4 days) significantly promotes the proliferation of LPS853 cells.
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J Biol Chem
2026 Apr;302(4):111345. PMID: 41791708 -
Eur J Immunol
Hyperinflammation by Human Macrophages Induced by SARS-CoV-2 Anti-Spike IgG Is Dependent on Glucose and Fatty Acid Metabolism. [Abstract]2025 Dec;55(12):e70087. PMID: 41416928 -
Eur J Immunol
2025 Oct;55(10):e70068. PMID: 41054024
6-Aminonicotinamide purchased from MedChemExpress. Usage Cited in: Eur J Immunol. 2025 Oct;55(10):e70068. [Abstract]
Alveolar-like macrophages co-stimulated with Poly(I:C) and anti-spike IgA were tested for their dependency on glycolysis, the pentose phosphate pathway, and fatty acid metabolism. Cells preincubated with metabolic inhibitors (6-Aminonicotinamide; 6-AN; 0.1 mM; 24 h) were stimulated and analyzed for their production of IL-6.
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J Photochem Photobiol B
14-3-3σ alleviates UVB-induced epidermal oxidative stress through the pentose phosphate pathway promotion. [Abstract]2026 Jun:279:113441. PMID: 42001822 -
bioRxiv
2026 Apr 4:2026.04.03.716381. PMID: 41959418 -
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Solvant et solubilité
DMSO : 100 mg/mL (729.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 11.11 mg/mL (81.01 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (18.23 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 4 mg/mL (29.17 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (274 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
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- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Cheng J, et al. TRIM21 and PHLDA3 negatively regulate the crosstalk between the PI3K/AKT pathway and PPP metabolism. Nat Commun. 2020;11(1):1880. Published 2020 Apr 20. [Content Brief]
[2]. Almugadam SH, et al. Influence of 6-aminonicotinamide (6AN) on Leishmania promastigotes evaluated by metabolomics: Beyond the pentose phosphate pathway. Chem Biol Interact. 2018;294:167-177. [Content Brief]
[3]. Tsouko E, et al. Regulation of the pentose phosphate pathway by an androgen receptor-mTOR-mediated mechanism and its role in prostate cancer cell growth. Oncogenesis. 2014;3(5):e103. Published 2014 May 26. [Content Brief]
[4]. Koutcher JA, et al. Effect of 6-aminonicotinamide on the pentose phosphate pathway: 31P NMR and tumor growth delay studies. Magn Reson Med. 1996;36(6):887-892. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 7.2917 mL | 36.4586 mL | 72.9171 mL | 182.2928 mL |
| 5 mM | 1.4583 mL | 7.2917 mL | 14.5834 mL | 36.4586 mL | |
| 10 mM | 0.7292 mL | 3.6459 mL | 7.2917 mL | 18.2293 mL | |
| 15 mM | 0.4861 mL | 2.4306 mL | 4.8611 mL | 12.1529 mL | |
| 20 mM | 0.3646 mL | 1.8229 mL | 3.6459 mL | 9.1146 mL | |
| 25 mM | 0.2917 mL | 1.4583 mL | 2.9167 mL | 7.2917 mL | |
| 30 mM | 0.2431 mL | 1.2153 mL | 2.4306 mL | 6.0764 mL | |
| 40 mM | 0.1823 mL | 0.9115 mL | 1.8229 mL | 4.5573 mL | |
| 50 mM | 0.1458 mL | 0.7292 mL | 1.4583 mL | 3.6459 mL | |
| 60 mM | 0.1215 mL | 0.6076 mL | 1.2153 mL | 3.0382 mL | |
| 80 mM | 0.0911 mL | 0.4557 mL | 0.9115 mL | 2.2787 mL | |
| DMSO | 100 mM | 0.0729 mL | 0.3646 mL | 0.7292 mL | 1.8229 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
6-Aminonicotinamide Related Classifications
HY-W010342 Related Classifications
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