Chloramphenicol succinate sodium

Name: Chloramphenicol succinate sodium
Brand: MedChemExpress
SKU: HY-N7114A
Rating: 5.0 (3 reviews)

Cat. No.: HY-N7114A Purity: 97.17%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Chloramphenicol succinate sodium is a prodrug of Chloramphenicol (HY-B0239), acting as a P2Y14R inhibitor with an IC₅₀ of 1.585 nM. Chloramphenicol succinate sodium serves as a competitive substrate and inhibitor of succinate dehydrogenase (SDH), which may account for its toxicity. Chloramphenicol succinate sodium exerts a significant inhibitory effect on colitis. Chloramphenicol succinate sodium can be used in research related to myelosuppression, gray baby syndrome, aplastic anemia, bacterial meningitis and inflammatory bowel disease.

For research use only. We do not sell to patients.

Chloramphenicol succinate sodium Chemical Structure

CAS No. : 982-57-0

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Chloramphenicol succinate sodium:

Chloramphenicol succinate Get quote
Chloramphenicol succinate sodium (Standard) Get quote

3 Publications Citing Use of MCE Chloramphenicol succinate sodium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All P2Y Receptor Isoform Specific Products:

View All Isoforms

P2Y1 Receptor P2Y2 Receptor P2Y6 Receptor P2Y11 Receptor P2Y12 Receptor P2Y13 Receptor P2Y14 Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[3]

P2Y14 Receptor

1.585 nM (IC₅₀)

In Vitro

Sodium chloramphenicol succinate (50 μM; 3-24 h) is slowly oxidized to chloramphenicol by in vitro human bone marrow mononuclear cells within 24 h^[1].
Chloramphenicol succinate (50-100 μM; 30-60 min) sodium is oxidized to Chloramphenicol by mitochondria isolated from human liver, rat liver and rat kidney within 60 min^[1].
Chloramphenicol succinate sodium is hydrolyzed by esterases present in the liver, kidney and lung of humans and animals, but not by blood esterases^[2].
Chloramphenicol succinate (0.01-100 μM; 24 h) sodium exhibits only extremely low cytotoxicity against HT-29 cells at concentrations up to 100 μM with a 24 h incubation^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Chloramphenicol succinate (50-100 μM; administered rectally; once daily for 7 consecutive days) sodium significantly alleviates DSS (HY-116282C)-induced colitis in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 J (male, 7-8-week-old, DSS-induced colitis)^[3]
Dosage:	50 μM; 100 μM
Administration:	rectal; daily; 7 days
Result:	Significantly attenuated DSS-induced colitis symptoms. Reduced weight loss. Lowered Disease Activity Index (DAI) scores. Prevented colon shortening. Mitigated colon tissue damage and inflammatory cell infiltration. Enhanced gut barrier integrity via increased expression of tight junction proteins (Claudin-1, Occludin, ZO-1).

Molecular Weight

445.18

Formula

C₁₅H₁₅Cl₂N₂NaO₈

CAS No.

982-57-0

Appearance

Solid

Color

White to light yellow

SMILES

O=C(CCC(O[Na])=O)OC[C@H]([C@@H](C1=CC=C(C=C1)[N+]([O-])=O)O)NC(C(Cl)Cl)=O

Structure Classification

Initial Source

Microorganisms

Streptomyces venequelae

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (280.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (112.31 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2463 mL	11.2314 mL	22.4628 mL
5 mM	0.4493 mL	2.2463 mL	4.4926 mL
10 mM	0.2246 mL	1.1231 mL	2.2463 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (224.63 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (278 KB) SDS (419 KB)

COA (250 KB) HNMR (199 KB)

Handling Instructions (2659 KB)

References

[1]. Ambekar CS, et al. Chloramphenicol succinate, a competitive substrate and inhibitor of succinate dehydrogenase: possible reason for its toxicity. Toxicol In Vitro. 2004;18(4):441-447. [Content Brief]

[2]. Ambrose PJ, et al. Clinical pharmacokinetics of chloramphenicol and chloramphenicol succinate. Clin Pharmacokinet. 1984;9(3):222-238. [Content Brief]

[3]. Tian S, et al. Computational discovery and repurposing of chloramphenicol succinate as a potent P2Y₁₄ receptor antagonist for inflammatory bowel disease therapy. J Adv Res. Published online August 22, 2025. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2463 mL	11.2314 mL	22.4628 mL	56.1571 mL
	5 mM	0.4493 mL	2.2463 mL	4.4926 mL	11.2314 mL
	10 mM	0.2246 mL	1.1231 mL	2.2463 mL	5.6157 mL
	15 mM	0.1498 mL	0.7488 mL	1.4975 mL	3.7438 mL
	20 mM	0.1123 mL	0.5616 mL	1.1231 mL	2.8079 mL
	25 mM	0.0899 mL	0.4493 mL	0.8985 mL	2.2463 mL
	30 mM	0.0749 mL	0.3744 mL	0.7488 mL	1.8719 mL
	40 mM	0.0562 mL	0.2808 mL	0.5616 mL	1.4039 mL
	50 mM	0.0449 mL	0.2246 mL	0.4493 mL	1.1231 mL
	60 mM	0.0374 mL	0.1872 mL	0.3744 mL	0.9360 mL
	80 mM	0.0281 mL	0.1404 mL	0.2808 mL	0.7020 mL
	100 mM	0.0225 mL	0.1123 mL	0.2246 mL	0.5616 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.