Citric acid trisodium
Based on 13 publication(s) in Google Scholar
Citric acid trisodium (Sodium citrate) is a natural preservative and food tartness enhancer. Citric acid trisodium induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Citric acid trisodium cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid trisodium is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 68-04-2
- Formula: C6H5Na3O7
- Molecular Weight:258.07
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Storage:
Store at room temperature, keep dry and cool.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Citric acid trisodium
More- Nature. 2025 Jul;643(8070):192-200. [Abstract]
- Mol Cancer. 2025 Oct 2;24(1):238. [Abstract]
- Cell Rep Med. 2026 Feb 17;7(2):102621. [Abstract]
- Food Chem. 2025 Oct 15:489:144992. [Abstract]
- Food Chem. 2023 Mar 30;405(Pt A):134807. [Abstract]
- Food Res Int. 2026 Feb 6.
- Regen Biomater. 2024 Aug 22:11:rbae098. [Abstract]
- Life Sci. 2024 Jun 15:347:122682. [Abstract]
- BMC Complement Med Ther. 2026 Apr 2;26(1):177. [Abstract]
- Insect Biochem Mol Biol. 2023 Jun:157:103958. [Abstract]
- Bone. 2025 Apr:193:117411. [Abstract]
- J Sep Sci. 2025 Nov;48(11):e70312. [Abstract]
- New J Chem. 03 Aug 2022.
All Endogenous Metabolite Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
>100 μM
Compound: Sodium citrate
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Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
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[PMID: 37360398] |
| HepG2 | IC50 |
>100 μM
Compound: Sodium citrate
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Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
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[PMID: 37360398] |
| HGC-27 | IC50 |
>100 μM
Compound: Sodium citrate
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Antiproliferative activity against human HGC-27 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
Antiproliferative activity against human HGC-27 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
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[PMID: 37360398] |
| L02 | IC50 |
>100 μM
Compound: Sodium citrate
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Cytotoxicity against human L02 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
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[PMID: 37360398] |
| MDA-MB-231 | IC50 |
>100 μM
Compound: Sodium citrate
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Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
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[PMID: 37360398] |
| SMMC-7721 | IC50 |
>100 μM
Compound: Sodium citrate
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Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
Antiproliferative activity against human SMMC-7721 cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
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[PMID: 37360398] |
Citric acid (0-12.5 mM; 24 h) trisodium shows antiproliferative activity in a dose dependent manner[3].
Citric acid (12.5 mM; 72 h) trisodium induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner[3].
Citric acid (12.5 mM; 48 h) trisodium increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HaCaT cells
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Concentration:0, 2.5, 5, 7.5, 10, 12.5 mM
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Incubation Time:24 h
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Result:Inhibited the cell viability in a dose dependent manner.
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Cell Line:HaCaT cells
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Concentration:12.5 mM
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Incubation Time:0, 12, 24, 48, 72 h
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Result:Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner.
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Cell Line:HaCaT cells
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Concentration:12.5 mM
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Incubation Time:12, 24, 48 h
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Result:Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.
Citric acid (120, 240, and 480 mg/kg; i.p.) trisodium induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:20 g male Kunming mice[2]
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Dosage:120, 240, 480 mg/kg
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Administration:I.p.; weekly for 3 weeks
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Result:T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.
Chemical Information
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CAS No. 68-04-2
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Appearance Solid
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Molecular Weight 258.07
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Formula C6H5Na3O7
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Color White to off-white
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SMILES
[Na].O=C(O)CC(O)(C(=O)O)CC(=O)O
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Synonyms
Sodium citrate; Trisodium citrate anhydrous
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature, keep dry and cool
In solvent -80°C 1 year -20°C 6 months
Publications (13)
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Journal Impact Factor
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Most Recent
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Nature
2025 Jul;643(8070):192-200. PMID: 39695227 -
Mol Cancer
Hsa_circ_0038737 promotes PARPi resistance in castration-resistant prostate cancer via IGF2BP3-mediated DNPH1 mRNA stabilization. [Abstract]2025 Oct 2;24(1):238. PMID: 41039575 -
Cell Rep Med
Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancer. [Abstract]2026 Feb 17;7(2):102621. PMID: 41707647 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 Oct 15:489:144992. PMID: 40466530 -
Food Chem
Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]2023 Mar 30;405(Pt A):134807. PMID: 36370576 -
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Regen Biomater
Polyzwitterion-grafted decellularized bovine intercostal arteries as new substitutes of small-diameter arteries for vascular regeneration. [Abstract]2024 Aug 22:11:rbae098. PMID: 39224131 -
Life Sci
Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest. [Abstract]2024 Jun 15:347:122682. PMID: 38702025 -
BMC Complement Med Ther
Hepatotoxicity prediction for traditional Chinese medicine: a two-step in silico framework integrating network and machine learning approaches. [Abstract]2026 Apr 2;26(1):177. PMID: 41923057 -
Insect Biochem Mol Biol
Development of an efficient insecticide substrate and inhibitor screening system of insect P450s using fission yeast. [Abstract]2023 Jun:157:103958. PMID: 37182814 -
Bone
The protection of nicotinamide riboside against diabetes mellitus-induced bone loss via OXPHOS. [Abstract]2025 Apr:193:117411. PMID: 39884488 -
J Sep Sci
Cell Metabolomics Reveals the Hepatotoxic Mechanism of Copper in Normal Rat Liver Cells Using Reversed-Phase and Hydrophilic Interaction Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry. [Abstract]2025 Nov;48(11):e70312. PMID: 41172059 -
Solvent & Solubility
H2O : 50 mg/mL (193.75 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (280 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen X, et al. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014 Mar;66(2):275-82. [Content Brief]
[2]. Chen X, Lv Q, Liu Y, Deng W. Effects of the food additive, citric acid, on kidney cells of mice. Biotech Histochem. 2015 Jan;90(1):38-44. [Content Brief]
[3]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]
[4]. Ciriminna R, et al. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017 Mar 8;11:22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.8749 mL | 19.3746 mL | 38.7492 mL | 96.8729 mL |
| 5 mM | 0.7750 mL | 3.8749 mL | 7.7498 mL | 19.3746 mL | |
| 10 mM | 0.3875 mL | 1.9375 mL | 3.8749 mL | 9.6873 mL | |
| 15 mM | 0.2583 mL | 1.2916 mL | 2.5833 mL | 6.4582 mL | |
| 20 mM | 0.1937 mL | 0.9687 mL | 1.9375 mL | 4.8436 mL | |
| 25 mM | 0.1550 mL | 0.7750 mL | 1.5500 mL | 3.8749 mL | |
| 30 mM | 0.1292 mL | 0.6458 mL | 1.2916 mL | 3.2291 mL | |
| 40 mM | 0.0969 mL | 0.4844 mL | 0.9687 mL | 2.4218 mL | |
| 50 mM | 0.0775 mL | 0.3875 mL | 0.7750 mL | 1.9375 mL | |
| 60 mM | 0.0646 mL | 0.3229 mL | 0.6458 mL | 1.6145 mL | |
| 80 mM | 0.0484 mL | 0.2422 mL | 0.4844 mL | 1.2109 mL | |
| 100 mM | 0.0387 mL | 0.1937 mL | 0.3875 mL | 0.9687 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Citric acid trisodium Related Classifications
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