Cat. No.: HY-B1610

Sodium citrate dihydrate

(Synonyms: Trisodium citrate dihydrate; Citric acid trisodium salt dihydrate)

14 Cited Publications

Customer Review

Based on 14 publication(s) in Google Scholar

Sodium citrate dehydrate (Trisodium citrate dihydrate) is a natural product with oral activity that can be found in citrus fruits. Sodium citrate dehydrate can inhibit the proliferation of tumor cells and induce apoptosis. Sodium citrate dehydrate has antibacterial, anti-tumor and antioxidant activities. Sodium citrate dehydrate can be prepared as a cosolvent or buffer.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 6132-04-3
Formula: C₆H₉Na₃O₉
Molecular Weight:294.10

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Sodium citrate dihydrate Chemical Structure

CAS No.: 6132-04-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (14)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Sodium citrate dehydrate (100 μM) can enhance the inhibition effect of rosemary acid (RA) on Vibrio bacterial^[1].
Sodium citrate dehydrate (5-20 mM, 24-48 h) induces apoptosis of human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

MGC-803
Concentration:

1.25, 2.5, 5, 10,15, 20, 30, 40, 60 mM
Incubation Time:

24, 48 h
Result:

Decreased cell viability in a dose-and time-dependent manner.
The IC₅₀ value is 10.08 mM after 48 h.

Apoptosis Analysis^[2]

Cell Line:

MGC-803
Concentration:

5, 10, 20 mM
Incubation Time:

24 h
Result:

Decreased the mRNA and protein expressions of anti-apoptotic Bcl-2 and Survivin.
Increased the mRNA and protein expression level of proapoptotic Bax, caspase-3 and Cyt-c.

Western Blot Analysis^[2]

Cell Line:

MGC-803
Concentration:

5, 10, 20 mM
Incubation Time:

24, 48 h
Result:

Induced cell apoptosis in a dose-and time-dependent manner.
Increased the G2/M phase arrest.

In Vivo

Sodium citrate dehydrate (216, 746 mg/kg, gavage for 16 weeks) can inhibit endoplasmic reticulum stress in rats with chronic renal failure induced by adenine (HY-B0152)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Adenine-Induced Chronic Renal Failure Rat Model^[3]
Dosage:

216, 746 mg/kg
Administration:

i.g.
Result:

Decreased the expression of renal fibrosis-related proteins TGF-β1 and CIV.
Decreased the expression of PERK, ATF6 and CHOP.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 6132-04-3
Appearance Solid
Molecular Weight 294.10
Formula C₆H₉Na₃O₉
Color White to off-white
SMILES

O=C(CC(C(O[Na])=O)(O)CC(O[Na])=O)O[Na].O.O
Synonyms

Trisodium citrate dihydrate; Citric acid trisodium salt dihydrate
Structure Classification
- Ketones, Aldehydes, Acids
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (14)

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Journal Impact Factor

Nature

Lithocholic acid phenocopies anti-ageing effects of calorie restriction. [Abstract]

2025 Jul;643(8070):192-200. PMID: 39695227
Mol Cancer

Hsa_circ_0038737 promotes PARPi resistance in castration-resistant prostate cancer via IGF2BP3-mediated DNPH1 mRNA stabilization. [Abstract]

2025 Oct 2;24(1):238. PMID: 41039575
J Exp Clin Cancer Res

Genomic profiling of a collection of patient-derived xenografts and cell lines identified ixabepilone as an active drug against chemo-resistant osteosarcoma. [Abstract]

2025 Jul 8;44(1):195. PMID: 40624718
Cell Rep Med

Bacterial vesicles from intratumoral L. salivarius enhance PD-1 blockade via FPR1-mediated macrophage polarization in gastric cancer. [Abstract]

2026 Feb 17;7(2):102621. PMID: 41707647
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Food Chem

Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]

2023 Mar 30;405(Pt A):134807. PMID: 36370576
Food Res Int

2026 Feb 6.
Regen Biomater

Polyzwitterion-grafted decellularized bovine intercostal arteries as new substitutes of small-diameter arteries for vascular regeneration. [Abstract]

2024 Aug 22:11:rbae098. PMID: 39224131
Life Sci

Homogentisic acid metabolism inhibits papillary thyroid carcinoma proliferation through ROS and p21-induced cell cycle arrest. [Abstract]

2024 Jun 15:347:122682. PMID: 38702025
BMC Complement Med Ther

Hepatotoxicity prediction for traditional Chinese medicine: a two-step in silico framework integrating network and machine learning approaches. [Abstract]

2026 Apr 2;26(1):177. PMID: 41923057
Insect Biochem Mol Biol

Development of an efficient insecticide substrate and inhibitor screening system of insect P450s using fission yeast. [Abstract]

2023 Jun:157:103958. PMID: 37182814
Bone

The protection of nicotinamide riboside against diabetes mellitus-induced bone loss via OXPHOS. [Abstract]

2025 Jan 28:193:117411. PMID: 39884488
J Sep Sci

Cell Metabolomics Reveals the Hepatotoxic Mechanism of Copper in Normal Rat Liver Cells Using Reversed-Phase and Hydrophilic Interaction Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry. [Abstract]

2025 Nov;48(11):e70312. PMID: 41172059
New J Chem

03 Aug 2022.

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (425.03 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4002 mL

17.0010 mL

34.0020 mL

5 mM

0.6800 mL

3.4002 mL

6.8004 mL

10 mM

0.3400 mL

1.7001 mL

3.4002 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (340.02 mM); Clear solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lu P, et al. Rosmarinic Acid and Sodium Citrate Have a Synergistic Bacteriostatic Effect against Vibrio Species by Inhibiting Iron Uptake. Int J Mol Sci. 2021 Dec 1;22(23):13010. [Content Brief]

[2]. Guo X,et al. 3-Bromopyruvate and sodium citrate induce apoptosis in human gastric cancer cell line MGC-803 by inhibiting glycolysis and promoting mitochondria-regulated apoptosis pathway. Biochem Biophys Res Commun. 2016 Jun 17;475(1):37-43. [Content Brief]

[3]. Ou Y, et al. Sodium Citrate Inhibits Endoplasmic Reticulum Stress in Rats with Adenine-Induced Chronic Renal Failure. Am J Nephrol. 2015;42(1):14-21. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	3.4002 mL	17.0010 mL	34.0020 mL	85.0051 mL
	5 mM	0.6800 mL	3.4002 mL	6.8004 mL	17.0010 mL
	10 mM	0.3400 mL	1.7001 mL	3.4002 mL	8.5005 mL
	15 mM	0.2267 mL	1.1334 mL	2.2668 mL	5.6670 mL
	20 mM	0.1700 mL	0.8501 mL	1.7001 mL	4.2503 mL
	25 mM	0.1360 mL	0.6800 mL	1.3601 mL	3.4002 mL
	30 mM	0.1133 mL	0.5667 mL	1.1334 mL	2.8335 mL
	40 mM	0.0850 mL	0.4250 mL	0.8501 mL	2.1251 mL
	50 mM	0.0680 mL	0.3400 mL	0.6800 mL	1.7001 mL
	60 mM	0.0567 mL	0.2834 mL	0.5667 mL	1.4168 mL
	80 mM	0.0425 mL	0.2125 mL	0.4250 mL	1.0626 mL
	100 mM	0.0340 mL	0.1700 mL	0.3400 mL	0.8501 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Sodium citrate dihydrate

14 Cited Publications

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Sodium citrate dihydrate

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Publications Citing Use of MedChemExpress (MCE) Sodium citrate dihydrate

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (14)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Sodium citrate dihydrate Related Classifications

HY-B1610 Related Classifications