DMU-212
Based on 2 publication(s) in Google Scholar
DMU-212 is a methylated derivative of Resveratrol (HY-16561), with antimitotic, anti-proliferative, antioxidant and apoptosis promoting activities. DMU-212 induces mitotic arrest via induction of apoptosis and activation of ERK1/2 protein. DMU-212 has orally active.
For research use only. We do not sell to patients.
- Purity: 99.31%
- CAS No.: 134029-62-2
- Formula: C18H20O4
- Molecular Weight:300.35
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
Publications Citing Use of MedChemExpress (MCE) DMU-212
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Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| 786-0 | GI50 |
5.42 μM
Compound: DMU-212
|
Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human 786-0 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| A498 | GI50 |
0.74 μM
Compound: DMU-212
|
Growth inhibition of human A498 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human A498 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| A549 | ED50 |
1.18 μM
Compound: 6a
|
Cytotoxic concentration required to inhibit 50% cell growth in A-549 lung carcinoma cell lines
Cytotoxic concentration required to inhibit 50% cell growth in A-549 lung carcinoma cell lines
|
[PMID: 1875350] |
| A549 | GI50 |
3.7 μM
Compound: DMU-212
|
Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human A549/ATCC cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| ACHN | GI50 |
4.51 μM
Compound: DMU-212
|
Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human ACHN cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| CAKI-1 | GI50 |
3 μM
Compound: DMU-212
|
Growth inhibition of human Caki1 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human Caki1 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| CCRF-CEM | GI50 |
2.89 μM
Compound: DMU-212
|
Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human CCRF-CEM cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| COLO 205 | GI50 |
2.07 μM
Compound: DMU-212
|
Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human COLO205 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| DU-145 | GI50 |
4.23 μM
Compound: DMU-212
|
Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human DU145 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HCC 2998 | GI50 |
3.59 μM
Compound: DMU-212
|
Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HCC2998 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HCT-116 | GI50 |
3.23 μM
Compound: DMU-212
|
Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HCT116 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HCT-116 | IC50 |
0.53 μM
Compound: 10E
|
Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay
|
[PMID: 21215623] |
| HCT-116 | IC50 |
1.1 μM
Compound: DMU-212
|
Anticancer activity against human HCT-116 cells assessed as cell growth inhibition
Anticancer activity against human HCT-116 cells assessed as cell growth inhibition
|
[PMID: 32485531] |
| HCT-15 | GI50 |
2.82 μM
Compound: DMU-212
|
Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HCT15 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HepG2 | IC50 |
0.3 μM
Compound: 1, DMU- 212
|
Cytotoxicity against human HepG2 cells after 96 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 96 hrs by MTT assay
|
[PMID: 21497957] |
| HL-60(TB) | GI50 |
3.14 μM
Compound: DMU-212
|
Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HL-60(TB) cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HOP-62 | GI50 |
3.14 μM
Compound: DMU-212
|
Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HOP62 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HOP-92 | GI50 |
7.23 μM
Compound: DMU-212
|
Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HOP92 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| Hs-578T | GI50 |
3.09 μM
Compound: DMU-212
|
Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human Hs 578T cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| HT-29 | ED50 |
1.82 μM
Compound: 6a
|
Cytotoxic concentration required to inhibit 50% cell growth in HT-29 colon adenocarcinoma cell lines
Cytotoxic concentration required to inhibit 50% cell growth in HT-29 colon adenocarcinoma cell lines
|
[PMID: 1875350] |
| HT-29 | GI50 |
2.32 μM
Compound: DMU-212
|
Growth inhibition of human HT-29 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human HT-29 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| IGROV-1 | GI50 |
5.29 μM
Compound: DMU-212
|
Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human IGROV1 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| IMR-90 | IC50 |
0.16 μM
Compound: 10E
|
Cytotoxicity against human IMR90 cells after 24 hrs by MTT assay
Cytotoxicity against human IMR90 cells after 24 hrs by MTT assay
|
[PMID: 21215623] |
| KM12 | GI50 |
3.63 μM
Compound: DMU-212
|
Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human KM12 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| LOX IMVI | GI50 |
4.89 μM
Compound: DMU-212
|
Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human LOXIMVI cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| M14 | GI50 |
2.81 μM
Compound: DMU-212
|
Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human M14 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| MCF7 | ED50 |
1.05 μM
Compound: 6a
|
Cytotoxic concentration required to inhibit 50% cell growth in MCF-7 breast carcinoma cell lines
Cytotoxic concentration required to inhibit 50% cell growth in MCF-7 breast carcinoma cell lines
|
[PMID: 1875350] |
| MCF7 | GI50 |
1.66 μM
Compound: DMU-212
|
Growth inhibition of human MCF7 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MCF7 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| MCF7 | IC50 |
0.22 μM
Compound: 10E
|
Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
|
[PMID: 21215623] |
| MCF7 | IC50 |
0.3 μM
Compound: 1, DMU- 212
|
Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay
|
[PMID: 21497957] |
| MCF7 | IC50 |
4.8 nM
Compound: Table 1, R13C1
|
Antiproliferative activity against human MCF7 cells after 48 hrs by sulforhodamine B assay
Antiproliferative activity against human MCF7 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 19135763] |
| MDA-MB-231 | GI50 |
3.74 μM
Compound: DMU-212
|
Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MDA-MB-231 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| MDA-MB-435 | GI50 |
1.04 μM
Compound: DMU-212
|
Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MDA-MB-435 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| MDA-MB-468 | GI50 |
2.22 μM
Compound: DMU-212
|
Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MDA-MB-468 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| MLM | ED50 |
2.07 μM
Compound: 6a
|
Cytotoxic concentration required to inhibit 50% cell growth in MLM melanoma cell lines
Cytotoxic concentration required to inhibit 50% cell growth in MLM melanoma cell lines
|
[PMID: 1875350] |
| MOLT-4 | GI50 |
3.22 μM
Compound: DMU-212
|
Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human MOLT4 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| NCI/ADR-RES | GI50 |
3.01 μM
Compound: DMU-212
|
Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-ADR-RES cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| NCI-H23 | GI50 |
3.37 μM
Compound: DMU-212
|
Growth inhibition of human NCI-H23 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-H23 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| NCI-H522 | GI50 |
3.7 μM
Compound: DMU-212
|
Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human NCI-H522 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| OVCAR-3 | GI50 |
3.45 μM
Compound: DMU-212
|
Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human OVCAR3 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| OVCAR-4 | GI50 |
4 μM
Compound: DMU-212
|
Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human OVCAR4 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| PC-3 | GI50 |
3.22 μM
Compound: DMU-212
|
Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| RPMI-8226 | GI50 |
6.42 μM
Compound: DMU-212
|
Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human RPMI8226 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SF-268 | GI50 |
7.59 μM
Compound: DMU-212
|
Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SF268 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SF-295 | GI50 |
2.18 μM
Compound: DMU-212
|
Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SF295 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SF-539 | GI50 |
2.18 μM
Compound: DMU-212
|
Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SF539 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SK-MEL-2 | GI50 |
3.95 μM
Compound: DMU-212
|
Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SK-MEL-2 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SK-MEL-28 | GI50 |
3.86 μM
Compound: DMU-212
|
Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SK-MEL-28 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SK-MEL-5 | ED50 |
0.81 μM
Compound: 6a
|
Cytotoxic concentration required to inhibit 50% cell growth in SKMEL-5 melanoma cell lines
Cytotoxic concentration required to inhibit 50% cell growth in SKMEL-5 melanoma cell lines
|
[PMID: 1875350] |
| SK-MEL-5 | GI50 |
2.5 μM
Compound: DMU-212
|
Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SK-MEL-5 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SK-OV-3 | GI50 |
3.34 μM
Compound: DMU-212
|
Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SKOV3 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SNB-19 | GI50 |
4.85 μM
Compound: DMU-212
|
Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SNB19 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SNB-75 | GI50 |
1.88 μM
Compound: DMU-212
|
Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SNB75 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SR | GI50 |
3.93 μM
Compound: DMU-212
|
Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SR cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| SW-620 | GI50 |
2.07 μM
Compound: DMU-212
|
Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human SW620 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| U-251 | GI50 |
3.07 μM
Compound: DMU-212
|
Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human U251 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| UACC-62 | GI50 |
2.37 μM
Compound: DMU-212
|
Growth inhibition of human UACC62 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human UACC62 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
| UO-31 | GI50 |
3.69 μM
Compound: DMU-212
|
Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay
Growth inhibition of human UO31 cells after 48 hrs by sulforhodamine B assay
|
[PMID: 27017113] |
DMU-212 (0.3125-40 μM) inhibits growth of A375, MeWo, Bro and M5 human melanoma cells[1].
DMU-212 (30-50 μM; 24 hours) induces upregulation of cell cycle inhibitors, apoptosis and ERK activation in A375 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:A375 cells, MeWo cells, M5 cells, Bro cells
-
Concentration:0.3125 μM, 0,625 μM, 1.25 μM, 2.5 μM, 5 μM, 10 μM, 20 μM, 40 μM
-
Incubation Time:96 hours
-
Result:Inhibited the cellular proliferation of human melanoma cells at submicromolar or micromolar concentrations (IC50=0.5 μM for A375 and Bro and IC50= 1.25 μM for MeWo and M5 cells).
-
Cell Line:A375 cells
-
Concentration:20 μM, 30 μM, 50 μM
-
Incubation Time:24 hours
-
Result:Caused a marked increase in the levels of p21, p53 and cyclin B1 proteins with a concomitant decrease in the levels of cyclin A2.
-
Cell Line:A375 cells
-
Concentration:20 μM, 30 μM, 50 μM
-
Incubation Time:24 hours
-
Result:Significant upregulated Bax, caspase 3 and caspase 9 protein levels, while decreased the levels of the anti-apoptotic protein Bcl-2.
-
Cell Line:A375 cells
-
Concentration:10 μM, 20 μM
-
Incubation Time:24 hours, 36 hours
-
Result:Induced apoptosis.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:6-weeks-old SCID female mice (20-24 g), with ovarian cancer xenografts[2]
-
Dosage:50 mg/kg
-
Administration:Oral gavage, three times a week, for 14 days
-
Result:Lowered tumor burden.
Chemical Information
-
CAS No. 134029-62-2
-
Appearance Solid
-
Molecular Weight 300.35
-
Formula C18H20O4
-
Color White to off-white
-
SMILES
COC1=CC=C(/C=C/C2=CC(OC)=C(OC)C(OC)=C2)C=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
-
Most Recent
-
Nat Commun
Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions. [Abstract]2024 Sep 12;15(1):7996. PMID: 39266508 -
J Neurosci
GRM2 Regulates Functional Integration of Adult-Born DGCs by Paradoxically Modulating MEK/ERK1/2 Pathway. [Abstract]2023 Apr 19;43(16):2822-2836. PMID: 36878727
Solvent & Solubility
DMSO : 20 mg/mL (66.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
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- Español - ES (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Vasilis Pericles Androutsopoulos, et al. Activation of ERK1/2 is required for the antimitotic activity of the resveratrol analogue 3,4,5,4'-tetramethoxystilbene (DMU-212) in human melanoma cells. Exp Dermatol. 2015 Aug;24(8):632-4. [Content Brief]
[2]. Hanna Piotrowska, et al. DMU-212 inhibits tumor growth in xenograft model of human ovarian cancer. Biomed Pharmacother. 2014 May;68(4):397-400. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.3294 mL | 16.6472 mL | 33.2945 mL | 83.2362 mL |
| 5 mM | 0.6659 mL | 3.3294 mL | 6.6589 mL | 16.6472 mL | |
| 10 mM | 0.3329 mL | 1.6647 mL | 3.3294 mL | 8.3236 mL | |
| 15 mM | 0.2220 mL | 1.1098 mL | 2.2196 mL | 5.5491 mL | |
| 20 mM | 0.1665 mL | 0.8324 mL | 1.6647 mL | 4.1618 mL | |
| 25 mM | 0.1332 mL | 0.6659 mL | 1.3318 mL | 3.3294 mL | |
| 30 mM | 0.1110 mL | 0.5549 mL | 1.1098 mL | 2.7745 mL | |
| 40 mM | 0.0832 mL | 0.4162 mL | 0.8324 mL | 2.0809 mL | |
| 50 mM | 0.0666 mL | 0.3329 mL | 0.6659 mL | 1.6647 mL | |
| 60 mM | 0.0555 mL | 0.2775 mL | 0.5549 mL | 1.3873 mL |