Ferulic acid methyl ester
Based on 1 publication(s) in Google Scholar
Ferulic acid methyl ester (Methyl ferulate) is a derivative of ferulic acid, isolated from Stemona tuberosa, with anti-inflammatory and antioxidant properties. Ferulic acid methyl ester is a cell membrane and brain permeable compound, shows free radical scavenging ability, used in the research of neurodegenerative disorders. Ferulic acid methyl ester inhibits COX-2 expression, blocks p-p38 and p-JNK in primary bone marrow derived-macrophages.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 2309-07-1
- Formula: C11H12O4
- Molecular Weight:208.21
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Ferulic acid methyl ester
More
Biological Activity
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p38 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>20 μg/mL
Compound: 17
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Cytotoxicity against human A549 cells by MTT assay
Cytotoxicity against human A549 cells by MTT assay
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[PMID: 29975532] |
| A549 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay
|
[PMID: 22954735] |
| Calu-1 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay
Cytotoxicity against human Calu1 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| HeLa | IC50 |
5.55 μM
Compound: 1a
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Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay
Cytotoxicity against human HeLa cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| HepG2 | IC50 |
>20 μg/mL
Compound: 17
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Cytotoxicity against human HepG2 cells by MTT assay
Cytotoxicity against human HepG2 cells by MTT assay
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[PMID: 29975532] |
| HOP-62 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay
Cytotoxicity against human HOP62 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| KB | IC50 |
>200 μM
Compound: 1c
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Antitumor activity against KB cells by MTT assay
Antitumor activity against KB cells by MTT assay
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[PMID: 17387015] |
| LOX IMVI | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay
Cytotoxicity against human LOXIMVI cells after 72 hrs by alamar blue assay
|
[PMID: 22954735] |
| M14 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay
Cytotoxicity against human M14 cells after 72 hrs by alamar blue assay
|
[PMID: 22954735] |
| MDA-MB-231 | IC50 |
>20 μg/mL
Compound: 17
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Cytotoxicity against human MDA-MB-231 cells by MTT assay
Cytotoxicity against human MDA-MB-231 cells by MTT assay
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[PMID: 29975532] |
| NCI-H1299 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H1299 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H157 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H157 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H1792 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay
Cytotoxicity against human NCI-H1792 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
| NCI-H460 | IC50 |
>50 μM
Compound: 1a
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Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay
Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay
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[PMID: 22954735] |
Ferulic acid methyl ester (25 µg/mL) has no cytotoxic effects on BMDMs after treatment for 6 h, 18 h, 48 h[2].
Ferulic acid methyl ester (Methyl ferulate; 5, 10, 25 µg/mL) suppresses TNFα, IL6, IFNγ but not IL10, inhibits NO generation at 10 and 25 µg/mL, in primary bone marrow derived-macrophages (BMDMs)[2].
Ferulic acid methyl ester (25 µg/mL) inhibits COX-2 expression, blocks p-p38 and p-JNK[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary bone marrow derived-macrophages (BMDMs)
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Concentration:25 µg/mL
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Incubation Time:6 h, 18 h, 48 h
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Result:Showed no cytotoxic effects on BMDMs.
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Cell Line:RAW 246.7 cells
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Concentration:10 µg/mL and 25 µg/mL
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Incubation Time:For 1 h before stimulation with LPS
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Result:Significantly suppressed COX-2 expression at 25 µg/mL.
Chemical Information
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CAS No. 2309-07-1
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Appearance Solid
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Molecular Weight 208.21
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Formula C11H12O4
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Color Off-white to pink
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SMILES
O=C(/C=C/C1=CC=C(C(OC)=C1)O)OC
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Synonyms
Methyl ferulate
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Fish Shellfish Immunol
Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]2025 Aug 6:166:110633. PMID: 40769268
Solvent & Solubility
DMSO : ≥ 100 mg/mL (480.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (9.61 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sultana R. Ferulic acid ethyl ester as a potential therapy in neurodegenerative disorders. Biochim Biophys Acta. 2012 May;1822(5):748-52. [Content Brief]
[2]. Phuong NT, et al. Anti-inflammatory activity of methyl ferulate isolated from Stemona tuberosa Lour. Asian Pac J Trop Med. 2014 Sep;7S1:S327-31. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.8028 mL | 24.0142 mL | 48.0284 mL | 120.0711 mL |
| 5 mM | 0.9606 mL | 4.8028 mL | 9.6057 mL | 24.0142 mL | |
| DMSO | 10 mM | 0.4803 mL | 2.4014 mL | 4.8028 mL | 12.0071 mL |
| 15 mM | 0.3202 mL | 1.6009 mL | 3.2019 mL | 8.0047 mL | |
| 20 mM | 0.2401 mL | 1.2007 mL | 2.4014 mL | 6.0036 mL | |
| 25 mM | 0.1921 mL | 0.9606 mL | 1.9211 mL | 4.8028 mL | |
| 30 mM | 0.1601 mL | 0.8005 mL | 1.6009 mL | 4.0024 mL | |
| 40 mM | 0.1201 mL | 0.6004 mL | 1.2007 mL | 3.0018 mL | |
| 50 mM | 0.0961 mL | 0.4803 mL | 0.9606 mL | 2.4014 mL | |
| 60 mM | 0.0800 mL | 0.4002 mL | 0.8005 mL | 2.0012 mL | |
| 80 mM | 0.0600 mL | 0.3002 mL | 0.6004 mL | 1.5009 mL | |
| 100 mM | 0.0480 mL | 0.2401 mL | 0.4803 mL | 1.2007 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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