2. Apoptosis
  3. Apoptosis
  4. Escin

Escin, a natural compound of triterpenoid saponins that can be isolated from horse chestnut (Aesculus hippocastanum) seeds, can be used as a vasoprotective anti-inflammatory, anti-edematous and anti-nociceptive agent.

For research use only. We do not sell to patients.

CAS No. : 6805-41-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

Escin Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
Flow Cytometry

    Escin purchased from MedChemExpress. Usage Cited in: Stem Cells Int. 2025 Nov 21:2025:1115668.  [Abstract]

    CCK-8 was used to detect the activity of UCMSCs after treatment with different concentrations of Escin (10, 20, 30, 40 μM) at 72 h.

    Escin purchased from MedChemExpress. Usage Cited in: Stem Cells Int. 2025 Nov 21:2025:1115668.  [Abstract]

    Cell cycle distribution of UCMSCs after Escin treatment.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Escin, a natural compound of triterpenoid saponins that can be isolated from horse chestnut (Aesculus hippocastanum) seeds, can be used as a vasoprotective anti-inflammatory, anti-edematous and anti-nociceptive agent[1].

    In Vitro

    Escin (1 μM, 0-5 h) stimulates phosphorylation of the GR in CFTL-12 murine mast cells[1].
    Escin (0.1-1 µg/mL, 24 h) protects cells from OGD/R and rt-PA stimulaed cell damage in bEnd.3 cells[2].
    Escin (0-100 μg/mL, 12-48 h) induces cell cycle arrest and apoptosis, and inhibits tumor cell proliferation in HCT116 and HCT8 cells[5].
    Escin (0-80 μg/mL, 12-24 h) induces DNA damage, and upregulates p-ATM and γH2AX in HCT116 and HCT8 cells[5].
    Escin (0-50 μM, 24 h) decreases mitochondrial membrane potential and induces cytochrome C release via generation of ROS, and induces apoptosis of bladder cancer cells (T24 and J82 cells )[6].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Escin Related Antibodies

    Western Blot Analysis[2]

    Cell Line: OGD/R and rt-PA stimulated bEnd.3 cells
    Concentration: 0.1, 0.5 and 1 µg/mL
    Incubation Time: 24 h
    Result: Upregulated the expression of ZO-1 and Occludin.
    In Vivo

    Escin (1-5 mg/kg, p.o.) inhibits the allergic skin response in Porcine induced by Compound 48/80 (HY-115768)[1].
    Escin (0.5 and 1 mg/kg, i.v.) attenuates recombinant tissue plasminogen activator (rt-PA) induced hemorrhagic transformation (HT) in mice[2].
    Escin (10 mg/kg, p.o., 4 days) shows antioxidant, anti-inflammatory, antinecrotic, and anti-apoptotic effects against Con A-induced immune-mediated hepatitis in mice[3].
    Escin (5-20 mg/kg, p.o., once a day, after 6 weeks of diabetes induction, for next 4 weeks) alleviates peripheral neuropathy in Streptozotocin (HY-13753) induced diabetes in rats[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Con A-induced immune-mediated hepatitis mice[3]
    Dosage: 10 mg/kg
    Administration: p.o., 4 days
    Result: Decreased the peak activities of ALT (p < 0.01), AST (p < 0.001), and LDH.
    Ameliorated hepatic pathological abnormalities.
    Limited Con A-induced elevation in Ly6G-positive cell in liver.
    Clinical Trial
    Molecular Weight

    1131.25

    Formula

    C55H86O24
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    OC[C@]12[C@](CC(C)([C@H]([C@@H]2OC(C)=O)[R])C)([H])C3=CC[C@@]([C@@]4([C@@]([C@](C)([C@H](CC4)O[C@H]5[C@@H]([C@H]([C@@H]([C@H](O5)C(O)=O)O[C@@H]6O[C@@H]([C@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@@H]7O[C@@H]([C@H]([C@@H]([C@H]7O)O)O)CO)CO)([H])CC8)C)([H])[C@]8(C)[C@@]3(C[C@H]1O)C.CC(OC[C@]9%10[C@](CC(C)([C@H]([C@@H]%10O)[R])C)([H])C%11=CC[C@@]([C@@]%12([C@@]([C@](C)([C@H](CC%12)O[C@H]%13[C@@H]([C@H]([C@@H]([C@H](O%13)C(O)=O)O[C@@H]%14O[C@@H]([C@H]([C@@H]([C@H]%14O)O)O)CO)O)O[C@@H]%15O[C@@H]([C@H]([C@@H]([C@H]%15O)O)O)CO)CO)([H])CC%16)C)([H])[C@]%16(C)[C@@]%11(C[C@H]9O)C)=O.[O]C(/C(C)=C\C)=O.[O]C(/C(C)=C/C)=O.[O]C(/C(C)=C\C)=O.[O]C(/C(C)=C/C)=O.[Aescin A].[Aescin B].[(E)].[(Z)].[Aescin C].[Aescin D].[(Z)].[(E)]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (88.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.8840 mL 4.4199 mL 8.8398 mL
    5 mM 0.1768 mL 0.8840 mL 1.7680 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (4.42 mM); Clear solution

      This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 96.6%

    SDS (563 KB)

    COA (267 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.8840 mL 4.4199 mL 8.8398 mL 22.0994 mL
    5 mM 0.1768 mL 0.8840 mL 1.7680 mL 4.4199 mL
    10 mM 0.0884 mL 0.4420 mL 0.8840 mL 2.2099 mL
    15 mM 0.0589 mL 0.2947 mL 0.5893 mL 1.4733 mL
    20 mM 0.0442 mL 0.2210 mL 0.4420 mL 1.1050 mL
    25 mM 0.0354 mL 0.1768 mL 0.3536 mL 0.8840 mL
    30 mM 0.0295 mL 0.1473 mL 0.2947 mL 0.7366 mL
    40 mM 0.0221 mL 0.1105 mL 0.2210 mL 0.5525 mL
    50 mM 0.0177 mL 0.0884 mL 0.1768 mL 0.4420 mL
    60 mM 0.0147 mL 0.0737 mL 0.1473 mL 0.3683 mL
    80 mM 0.0110 mL 0.0552 mL 0.1105 mL 0.2762 mL
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    Escin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Escin6805-41-0Apoptosisanti-inflammatoryanti-edematousanti-nociceptivetumorInhibitorinhibitorinhibit

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