Farnesylthioacetic acid

Cat. No.: HY-118563: Data Sheet
COA

SDS
Handling Instructions
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Farnesylthioacetic acid is a competitive, non-substrate inhibitor of Prenylcysteine α-carboxyl methyltransferase. It acts as a non-substrate competitive inhibitor of Arabidopsis thaliana Prenylcysteine α-carboxyl methyltransferase and blocks methyltransferase activity. Farnesylthioacetic acid does not inhibit protein farnesyltransferase activity in Arabidopsis. It induces Apoptosis. Farnesylthioacetic acid regulates the subcellular localization of Ras protein, reducing the proportion of cytoplasmic Ras protein without disrupting membrane binding. It enhances ABA-induced seed dormancy, delays seed germination, and promotes maximum stomatal closure at lower exogenous ABA concentrations. Farnesylthioacetic acid can be used in studies related to promyelocytic leukemia.

For research use only. We do not sell to patients.

Farnesylthioacetic acid Chemical Structure

CAS No. : 135784-48-4

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5 mg		Ask For Quote & Lead Time
10 mg		Ask For Quote & Lead Time
25 mg		Ask For Quote & Lead Time

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	90 μM Compound: 11	Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels Agonist activity at human TRPA1 channel expressed in CHO cells assessed as increase in intracellular calcium levels	[PMID: 20356305]

In Vitro

Farnesylthioacetic acid (2-25 μM; 8 h-2 days) potently inhibits the growth of promyelocytic leukemia HL-60 cells, achieving complete inhibition at a concentration of 5 μM after 2 days, and induces dose-dependent apoptosis, which is characterized by accumulation of cells in sub-G1 phase, increased annexin V binding, and formation of DNA ladder bands^[1].
Farnesylthioacetic acid (10-100 μM; 30 min) potently inhibits methylation of the 20-23 kDa protein in HL-60 cell extracts, with an IC₅₀ of 30 μM, while it exhibits weak or no inhibitory effects on histones of other molecular weights^[1].
Farnesylthioacetic acid (10 μM; 8 h) increases the proportion of ras protein in the membrane fraction of promyelocytic leukemia HL-60 cells, while reducing the level of cytoplasmic ras protein, without altering the total abundance of ras protein^[1].
Farnesylthioacetic acid (100 μM) exhibits no inhibitory effect on protein farnesyltransferase activity in crude extracts of cultured Arabidopsis thaliana cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	human promyelocytic HL-60 leukemic cells
Concentration:	2 μM; 5 μM; 10 μM; 25 μM
Incubation Time:	8 h; 12 h; 2 days
Result:	Inhibited HL-60 cell growth in a dose-dependent manner, with complete inhibition at 5 μM after 2 days. Induced dose-dependent appearance of a sub-G1 cell population: 16% of cells were in sub-G1 with 5 μM, 26% with 10 μM, and 49% with 25 μM after 8 h. Increased annexin V-FITC binding in a dose-dependent manner, with early apoptotic cells making up 23% of the population with 2 μM, 22% with 5 μM, and 42% with 10 μM after treatment. Induced internucleosomal DNA degradation, forming the characteristic apoptotic DNA laddering pattern after incubation with 10 μM for 12 h.

Western Blot Analysis^[1]

Cell Line:	human promyelocytic HL-60 leukemic cells
Concentration:	10 μM
Incubation Time:	8 h
Result:	Did not reduce membrane-associated ras protein levels; instead, increased the proportion of ras in the membrane fraction and decreased the proportion in the cytosolic fraction. Did not alter total ras protein levels in whole cell lysates, and calmodulin subcellular distribution remained unchanged, confirming fractionation integrity.

Molecular Weight

296.47

Formula

C₁₇H₂₈O₂S

CAS No.

135784-48-4

Appearance

Viscous Liquid

Color

Colorless to light yellow

SMILES

C/C(C)=C\CC/C(C)=C/CC/C(C)=C/CSCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (337.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3730 mL	16.8651 mL	33.7302 mL
5 mM	0.6746 mL	3.3730 mL	6.7460 mL
10 mM	0.3373 mL	1.6865 mL	3.3730 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 90.0%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (239 KB) HNMR (132 KB) RP-HPLC (276 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Pérez-Sala D, et al. Analogs of farnesylcysteine induce apoptosis in HL-60 cells. FEBS Lett. 1998;426(3):319-324.

[2]. Narasimha Chary S, et al. Prenylcysteine alpha-carboxyl methyltransferase expression and function in Arabidopsis thaliana. Plant J. 2002;32(5):735-747.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3730 mL	16.8651 mL	33.7302 mL	84.3256 mL
	5 mM	0.6746 mL	3.3730 mL	6.7460 mL	16.8651 mL
	10 mM	0.3373 mL	1.6865 mL	3.3730 mL	8.4326 mL
	15 mM	0.2249 mL	1.1243 mL	2.2487 mL	5.6217 mL
	20 mM	0.1687 mL	0.8433 mL	1.6865 mL	4.2163 mL
	25 mM	0.1349 mL	0.6746 mL	1.3492 mL	3.3730 mL
	30 mM	0.1124 mL	0.5622 mL	1.1243 mL	2.8109 mL
	40 mM	0.0843 mL	0.4216 mL	0.8433 mL	2.1081 mL
	50 mM	0.0675 mL	0.3373 mL	0.6746 mL	1.6865 mL
	60 mM	0.0562 mL	0.2811 mL	0.5622 mL	1.4054 mL
	80 mM	0.0422 mL	0.2108 mL	0.4216 mL	1.0541 mL
	100 mM	0.0337 mL	0.1687 mL	0.3373 mL	0.8433 mL