Fraxetin

Name: Fraxetin
Brand: MedChemExpress
SKU: HY-N0580
Rating: 5.0 (2 reviews)

Cat. No.: HY-N0580 Purity: 99.96%: Data Sheet
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Fraxetin is isolated from Fraxinus rhynchophylla Hance and has oral bioactivity. Fraxetin has anti-tumor, antibacterial, antioxidant, and anti-inflammatory effects. Fraxetin can induce cell apoptosis.

For research use only. We do not sell to patients.

CAS No. : 574-84-5

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
20 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Fraxetin:

Fraxetin (Standard) Get quote

2 Publications Citing Use of MCE Fraxetin

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

Flow Cytometry

: Fraxetin purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jul 16;10(15):e34717.; Viability of THP1 and HL60 cells after 24h of Fraxetin (40, 80, 120, 160, 200 μM) treatment.

: Fraxetin purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jul 16;10(15):e34717.; THP1 and HL60 cells were treated with Fraxetin (0, 80, 120, 160 μM) for 24 h, apoptosis rates were analyzed using Annexin V-FITC/PI flow cytometry.

: Fraxetin purchased from MedChemExpress. Usage Cited in: Heliyon. 2024 Jul 16;10(15):e34717.; Cells were treated with Fraxetin (0, 80, 120, 160 μM) for 24 h, and the expression levels of apoptosis-related proteins Bax and Bcl-2 were analyzed by western blotting.

: Fraxetin purchased from MedChemExpress. Usage Cited in: Evid Based Complement Alternat Med. 2021 Apr 16:2021:5540139. [Abstract]; Fraxetin (100, 200 μM, 24 h) inhibits the invasion and migration of U251 cells.

: Fraxetin purchased from MedChemExpress. Usage Cited in: Evid Based Complement Alternat Med. 2021 Apr 16:2021:5540139. [Abstract]; Immunofluorescence analysis of STAT3. Cells were treated with Fraxetin (0 μM, 100 μM, and 200 μM) for 24 h.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HaCaT	IC₅₀	2.8 μM Compound: Fraxetin	Inhibition of TNFalpha production in UVB irradiated human HaCaT cells administered 2 hrs before irradiation by fluoroimmunoassay Inhibition of TNFalpha production in UVB irradiated human HaCaT cells administered 2 hrs before irradiation by fluoroimmunoassay	[PMID: 17317175]
Neutrophil	IC₅₀	1 μM Compound: fraxetin	Inhibition of TPA-induced ROS production in human neutrophils by chemiluminescence Inhibition of TPA-induced ROS production in human neutrophils by chemiluminescence	[PMID: 15387642]
U-937	CC₅₀	70.2 μM Compound: 2, Fraxetin	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]
U-937	IC₅₀	142.9 μM Compound: 2, Fraxetin	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]

In Vitro

Fraxetin (20-60 μM, 24 h) inhibits the proliferation of MCF-7 cells and induces apoptosis^[1].
Fraxetin (0.02-0.08 mg/ml, 30 min) disrupts the synthesis of nucleic acids and proteins by preventing topoisomerase from binding to DNA, showing significant inhibitory effects on the proliferation of Staphylococcus aureus^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MCF-7
Concentration:	20, 40, 60 μM
Incubation Time:	24 h
Result:	Inhibited cell proliferation.

Immunofluorescence^[1]

Cell Line:	MCF-7
Concentration:	20, 40, 60 μM
Incubation Time:	24 h
Result:	Condensed chromatin.

RT-PCR^[1]

Cell Line:	MCF-7
Concentration:	20, 40, 60 μM
Incubation Time:	24 h
Result:	Upregulated the expression of Fas and FasL mRNA.

Western Blot Analysis^[1]

Cell Line:	MCF-7
Concentration:	20, 40, 60 μM
Incubation Time:	24 h
Result:	Upregulated Bax and downregulated Bcl-2.

In Vivo

Fraxetin (20-50 mg/kg, gavage, once daily, 24 weeks) has a hepatoprotective effect in mice and also has antioxidant and anti-inflammatory activities^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ethanol-induced liver fibrosis model in rats ^[3]
Dosage:	20, 50 mg/kg; daily; 24 weeks
Administration:	i.g.
Result:	Reduced serum ALT and AST, weakened tissue pathological changes, upregulated heme oxygenase-1 (HO-1) protein, reduced lipid peroxidation, enhanced liver antioxidant capacity, inhibited CYP2E1 activity, and reduced inflammatory mediators such as TNF-α and IL-1β.

Molecular Weight

208.17

Formula

C₁₀H₈O₅

CAS No.

574-84-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=CC2=CC(OC)=C(O)C(O)=C2O1

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (480.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8038 mL	24.0188 mL	48.0377 mL
5 mM	0.9608 mL	4.8038 mL	9.6075 mL
10 mM	0.4804 mL	2.4019 mL	4.8038 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (9.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (120.09 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (250 KB)

Handling Instructions (2659 KB)

References

[1]. Liu G, et al. Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncol Lett. 2017 Dec;14(6):7374-7378. [Content Brief]

[2]. Haiting Wang, et al. Antibacterial mechanism of fraxetin against Staphylococcus aureus. Mol Med Rep. 2014 Nov;10(5):2341-5. [Content Brief]

[3]. Chen X, et al. The therapeutic effect of fraxetin on ethanol-induced hepatic fibrosis by enhancing ethanol metabolism, inhibiting oxidative stress and modulating inflammatory mediators in rats. Int Immunopharmacol. 2018 Mar;56:98-104. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8038 mL	24.0188 mL	48.0377 mL	120.0942 mL
	5 mM	0.9608 mL	4.8038 mL	9.6075 mL	24.0188 mL
	10 mM	0.4804 mL	2.4019 mL	4.8038 mL	12.0094 mL
	15 mM	0.3203 mL	1.6013 mL	3.2025 mL	8.0063 mL
	20 mM	0.2402 mL	1.2009 mL	2.4019 mL	6.0047 mL
	25 mM	0.1922 mL	0.9608 mL	1.9215 mL	4.8038 mL
	30 mM	0.1601 mL	0.8006 mL	1.6013 mL	4.0031 mL
	40 mM	0.1201 mL	0.6005 mL	1.2009 mL	3.0024 mL
	50 mM	0.0961 mL	0.4804 mL	0.9608 mL	2.4019 mL
	60 mM	0.0801 mL	0.4003 mL	0.8006 mL	2.0016 mL
	80 mM	0.0600 mL	0.3002 mL	0.6005 mL	1.5012 mL
	100 mM	0.0480 mL	0.2402 mL	0.4804 mL	1.2009 mL

Fraxetin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fraxetin574-84-5ApoptosisBacterialAnti-tumoranti-bacterialantioxidantanti-inflammatoryInhibitorinhibitorinhibit

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Fraxetin

Customer Review

Other Forms of Fraxetin:

Fraxetin Related Antibodies

2 Publications Citing Use of MCE Fraxetin

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Fraxetin Related Antibodies

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Complete Stock Solution Preparation Table

Fraxetin Related Classifications

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