Cat. No.: HY-121955

FW1256

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

FW1256 is a phenyl analogue and a slow-releasing hydrogen sulfide (H₂S) donor. FW1256 inhibits NF-κB activity and induces cell apoptosis. FW1256 exerts potent anti-inflammatory effects and has the potential for cancer and cardiovascular disease treatment.

For research use only. We do not sell to patients.

Purity: 95.0%
CAS No.: 117089-08-4
Formula: C₁₂H₁₀NOPS
Molecular Weight:247.25

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

FW1256 Chemical Structure

CAS No.: 117089-08-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Hepatocyte	IC₅₀	26.3 μM Compound: 22, FW1256	Toxicity in transforming growth factor alpha transgenic mouse hepatocytes assessed as inhibition of cell proliferation after 24 hrs by CellTiter-Glo cell viability assay Toxicity in transforming growth factor alpha transgenic mouse hepatocytes assessed as inhibition of cell proliferation after 24 hrs by CellTiter-Glo cell viability assay	[PMID: 26147240]
MCF7	IC₅₀	5.7 μM Compound: 22, FW1256	Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation after 4 days by crystal violet assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation after 4 days by crystal violet assay	[PMID: 26147240]
SK-OV-3	IC₅₀	6.12 μM Compound: 22, FW1256	Cytotoxicity against human SKOV3 cells assessed as inhibition of cell proliferation after 4 days by crystal violet assay Cytotoxicity against human SKOV3 cells assessed as inhibition of cell proliferation after 4 days by crystal violet assay	[PMID: 26147240]
SK-OV-3	IC₅₀	6.12 μM Compound: 3; FW1256	Antiproliferative activity against human SKOV3 cells after 4 days by crystal violet staining based spectrophotometric method Antiproliferative activity against human SKOV3 cells after 4 days by crystal violet staining based spectrophotometric method	[PMID: 28082040]
WI-38	IC₅₀	>50 μM Compound: 22, FW1256	Toxicity in human WI38 cells assessed as inhibition of cell proliferation after 4 days by crystal violet assay Toxicity in human WI38 cells assessed as inhibition of cell proliferation after 4 days by crystal violet assay	[PMID: 26147240]
WI-38	IC₅₀	>50 μM Compound: 3; FW1256	Antiproliferative activity against human WI38 cells after 4 days by crystal violet staining based spectrophotometric method Antiproliferative activity against human WI38 cells after 4 days by crystal violet staining based spectrophotometric method	[PMID: 28082040]

In Vitro

FW1256 (200 μM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS mRNA and protein in LPS-stimulated RAW264.7 macrophages^[1].
FW1256 (200 μM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS PROTE and protein in LPS-stimulated RAW264.7 macrophages^[1].
FW1256 concentration dependently decreases TNF-α (IC₅₀ of 61.2 μM), IL-6 (IC₅₀ of 11.7 μM), PGE2 (IC₅₀ of 25.5 μM) and NO (IC₅₀ of 34.6 μM) generation in LPS-stimulated RAW264.7 macrophages and bone marrow-derived macrophages (BMDMs) (IC₅₀s of 414.9 μM, 300.2 μM, 4 μM and 9.5 μM for TNF-α, IL-6, PGE2 and NO, respectively) ^[1].
FW1256 decreases NF-κB activation as evidenced by reduced cytosolic phospho-IκBα levels and reduces nuclear p65 levels in LPS-stimulated RAW264.7 macrophages treated with FW1256^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:

RAW264.7 cells
Concentration:

200 µM
Incubation Time:

24.5 hours
Result:

Significantly reduced IL-1β, COX-2 and iNOS mRNA in LPS-stimulated RAW264.7 macrophages..

Western Blot Analysis^[1]

Cell Line:

RAW264.7 cells
Concentration:

200 µM
Incubation Time:

24.5 hours
Result:

Significantly reduced IL-1β, COX-2 and iNOS proteinin LPS-stimulated RAW264.7 macrophages..

In Vivo

FW1256 (100 mg/kg; intraperitoneal injection; male C57BL/6 mice) treatment reduces IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6 mice (20-25 g, 6-10 weeks) injected with E. coli lipopolysaccharide (LPS)^[1]
Dosage:

100 mg/kg
Administration:

Intraperitoneal injection
Result:

Reduced IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice.

Chemical Information

CAS No. 117089-08-4
Appearance Solid
Molecular Weight 247.25
Formula C₁₂H₁₀NOPS
Color White to off-white
SMILES

S=P1(OC2=CC=CC=C2N1)C3=CC=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Publications (1)

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Journal Impact Factor

Am J Transl Res

Exosomes released from mesenchymal stem cells overexpressing microRNA-30e ameliorate heart failure in rats with myocardial infarction. [Abstract]

2021 May 15;13(5):4007-4025. PMID: 34149995

Solvent & Solubility

In Vitro:

DMSO : ≥ 250 mg/mL (1011.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0445 mL

20.2224 mL

40.4449 mL

5 mM

0.8089 mL

4.0445 mL

8.0890 mL

10 mM

0.4044 mL

2.0222 mL

4.0445 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Huang CW, et al. A novel slow-releasing hydrogen sulfide donor, FW1256, exerts anti-inflammatory effects in mouse macrophages and in vivo. Pharmacol Res. 2016 Nov;113(Pt A):533-546. [Content Brief]

[2]. Feng W, et al. Discovery of New H2S Releasing Phosphordithioates and 2,3-Dihydro-2-phenyl-2-sulfanylenebenzo[d][1,3,2]oxazaphospholes with Improved Antiproliferative Activity. J Med Chem. 2015 Aug 27;58(16):6456-80. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.0445 mL	20.2224 mL	40.4449 mL	101.1122 mL
	5 mM	0.8089 mL	4.0445 mL	8.0890 mL	20.2224 mL
	10 mM	0.4044 mL	2.0222 mL	4.0445 mL	10.1112 mL
	15 mM	0.2696 mL	1.3482 mL	2.6963 mL	6.7408 mL
	20 mM	0.2022 mL	1.0111 mL	2.0222 mL	5.0556 mL
	25 mM	0.1618 mL	0.8089 mL	1.6178 mL	4.0445 mL
	30 mM	0.1348 mL	0.6741 mL	1.3482 mL	3.3704 mL
	40 mM	0.1011 mL	0.5056 mL	1.0111 mL	2.5278 mL
	50 mM	0.0809 mL	0.4044 mL	0.8089 mL	2.0222 mL
	60 mM	0.0674 mL	0.3370 mL	0.6741 mL	1.6852 mL
	80 mM	0.0506 mL	0.2528 mL	0.5056 mL	1.2639 mL
	100 mM	0.0404 mL	0.2022 mL	0.4044 mL	1.0111 mL

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FW1256

Apoptosis Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Anti-Cardiovascular Disease Compound Library

Pyroptosis Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) FW1256

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

FW1256 Related Classifications

Cat. No.: HY-121955