2. NF-κB Apoptosis
  3. NF-κB Apoptosis
  4. FW1256

FW1256 is a phenyl analogue and a slow-releasing hydrogen sulfide (H2S) donor. FW1256 inhibits NF-κB activity and induces cell apoptosis. FW1256 exerts potent anti-inflammatory effects and has the potential for cancer and cardiovascular disease treatment.

For research use only. We do not sell to patients.

FW1256 Chemical Structure

FW1256 Chemical Structure

CAS No. : 117089-08-4

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Customer Review

Based on 1 publication(s) in Google Scholar

FW1256 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE FW1256

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

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Description

FW1256 is a phenyl analogue and a slow-releasing hydrogen sulfide (H2S) donor. FW1256 inhibits NF-κB activity and induces cell apoptosis. FW1256 exerts potent anti-inflammatory effects and has the potential for cancer and cardiovascular disease treatment[1][2].

In Vitro

FW1256 (200 µM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS mRNA and protein in LPS-stimulated RAW264.7 macrophages[1].
FW1256 (200 µM; 24.5 hours; AW264.7 cells) treatment significantly reduces IL-1β, COX-2 and iNOS PROTE and protein in LPS-stimulated RAW264.7 macrophages[1].
FW1256 concentration dependently decreases TNF-α (IC50 of 61.2 µM), IL-6 (IC50 of 11.7 µM), PGE2 (IC50 of 25.5 µM) and NO (IC50 of 34.6 µM) generation in LPS-stimulated RAW264.7 macrophages and bone marrow-derived macrophages (BMDMs) (IC50s of 414.9 µM, 300.2 µM, 4 µM and 9.5 µM for TNF-α, IL-6, PGE2 and NO, respectively) [1].
FW1256 decreases NF-κB activation as evidenced by reduced cytosolic phospho-IκBα levels and reduces nuclear p65 levels in LPS-stimulated RAW264.7 macrophages treated with FW1256[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FW1256 Related Antibodies

RT-PCR[1]

Cell Line: RAW264.7 cells
Concentration: 200 µM
Incubation Time: 24.5 hours
Result: Significantly reduced IL-1β, COX-2 and iNOS mRNA in LPS-stimulated RAW264.7 macrophages..

Western Blot Analysis[1]

Cell Line: RAW264.7 cells
Concentration: 200 µM
Incubation Time: 24.5 hours
Result: Significantly reduced IL-1β, COX-2 and iNOS proteinin LPS-stimulated RAW264.7 macrophages..
In Vivo

FW1256 (100 mg/kg; intraperitoneal injection; male C57BL/6 mice) treatment reduces IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (20-25 g, 6-10 weeks) injected with E. coli lipopolysaccharide (LPS)[1]
Dosage: 100 mg/kg
Administration: Intraperitoneal injection
Result: Reduced IL-1β, TNFα, nitrate/nitrite and PGE2 levels in LPS-treated mice.
Molecular Weight

247.25

Formula

C12H10NOPS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

S=P1(OC2=CC=CC=C2N1)C3=CC=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 250 mg/mL (1011.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0445 mL 20.2224 mL 40.4449 mL
5 mM 0.8089 mL 4.0445 mL 8.0890 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0445 mL 20.2224 mL 40.4449 mL 101.1122 mL
5 mM 0.8089 mL 4.0445 mL 8.0890 mL 20.2224 mL
10 mM 0.4044 mL 2.0222 mL 4.0445 mL 10.1112 mL
15 mM 0.2696 mL 1.3482 mL 2.6963 mL 6.7408 mL
20 mM 0.2022 mL 1.0111 mL 2.0222 mL 5.0556 mL
25 mM 0.1618 mL 0.8089 mL 1.6178 mL 4.0445 mL
30 mM 0.1348 mL 0.6741 mL 1.3482 mL 3.3704 mL
40 mM 0.1011 mL 0.5056 mL 1.0111 mL 2.5278 mL
50 mM 0.0809 mL 0.4044 mL 0.8089 mL 2.0222 mL
60 mM 0.0674 mL 0.3370 mL 0.6741 mL 1.6852 mL
80 mM 0.0506 mL 0.2528 mL 0.5056 mL 1.2639 mL
100 mM 0.0404 mL 0.2022 mL 0.4044 mL 1.0111 mL
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FW1256 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FW1256117089-08-4FW 1256FW-1256NF-κBApoptosisNuclear factor-κBNuclear factor-kappaBTNF-αIL-6H2SLPSanti-inflammatoryphenylPGE2NOnon-cytotoxicInhibitorinhibitorinhibit

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