Galegine hydrochloride
Galegine hydrochloride, a guanidine derivative, contributes to weight loss in mice. Guanidine hydrochloride is the compound derived from G. officinalis, which gave rise to the biguanides, metformin and phenformin. Galegine hydrochloride activates AMPK in 3T3-L1 adipocytes and L6 myotubes, as well as in the H4IIE rat hepatoma and HEK293 human kidney cell lines. Galegine hydrochloride has antibacterial activity, with minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains.
For research use only. We do not sell to patients.
- CAS No.: 2368870-39-5
- Formula: C6H14ClN3
- Molecular Weight:163.65
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Pre-treatment with Galegine hydrochloride (10?μM-3?mM; 5?h) produces a concentration-dependent stimulation of insulin-independent glucose uptake by 3T3-L1 adipocytes without any effect on cell viability. Incubation with Galegine hydrochloride (1?μM-1?mM, 5?h) produces a concentration-dependent stimulation of glucose uptake into L6 myotubes, again without any effect on cell viability[1].
Galegine hydrochloride (0.3-300?μM; 24 hours) produced a slight reduction in basal glycerol release and a more marked reduction in isoprenaline-stimulated glycerol release from 3T3-L1 adipocytes. Incubation of H4IIE rat hepatoma cells with Galegine hydrochloride (10 or 300?μM) for up to 6?hours produces a time-dependent activation of AMPK measured in cell lysates, with maximal activation at 360?min and twofold activation still evident at 24?hous in the presence of 300?μM Galegine hydrochloride. The effect of 300?ΜM Galegine hydrochloride is markedly greater than that of 10?μM. Incubation with Galegine hydrochloride for 1?hour produces a concentration-dependent activation of AMPK in both 3T3L-1 adipocytes and L-6 myotubes. Galegine hydrochloride also produces a concentration-dependent activation of the enzyme in a human kidney cell line (HEK293)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult male BALB/c mice[1]
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Dosage:63 mg/kg
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Administration:feed; daily for 11 days
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Result:Produced a significant reduction in body weight.
Chemical Information
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CAS No. 2368870-39-5
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Appearance Solid
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Molecular Weight 163.65
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Formula C6H14ClN3
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Color White to off-white
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SMILES
NC(NC/C=C(C)\C)=N.[H]Cl
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : 43.33 mg/mL (264.77 mM; Need ultrasonic)
DMSO : 5.2 mg/mL (31.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Mooney MH, et al. Mechanisms underlying the metabolic actions of galegine that contribute to weight loss in mice. Br J Pharmacol. 2008 Apr;153(8):1669-77. [Content Brief]
[2]. Coqueiro A, et al. In Vitro Antibacterial Activity of Prenylated Guanidine Alkaloids from Pterogyne nitens and Synthetic Analogues. J Nat Prod. 2014 Aug 22;77(8):1972-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 6.1106 mL | 30.5530 mL | 61.1060 mL | 152.7651 mL |
| 5 mM | 1.2221 mL | 6.1106 mL | 12.2212 mL | 30.5530 mL | |
| 10 mM | 0.6111 mL | 3.0553 mL | 6.1106 mL | 15.2765 mL | |
| 15 mM | 0.4074 mL | 2.0369 mL | 4.0737 mL | 10.1843 mL | |
| 20 mM | 0.3055 mL | 1.5277 mL | 3.0553 mL | 7.6383 mL | |
| 25 mM | 0.2444 mL | 1.2221 mL | 2.4442 mL | 6.1106 mL | |
| 30 mM | 0.2037 mL | 1.0184 mL | 2.0369 mL | 5.0922 mL | |
| H2O | 40 mM | 0.1528 mL | 0.7638 mL | 1.5277 mL | 3.8191 mL |
| 50 mM | 0.1222 mL | 0.6111 mL | 1.2221 mL | 3.0553 mL | |
| 60 mM | 0.1018 mL | 0.5092 mL | 1.0184 mL | 2.5461 mL | |
| 80 mM | 0.0764 mL | 0.3819 mL | 0.7638 mL | 1.9096 mL | |
| 100 mM | 0.0611 mL | 0.3055 mL | 0.6111 mL | 1.5277 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Galegine hydrochloride Related Classifications
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