Galegine hemisulfate

Name: Galegine hemisulfate
Brand: MedChemExpress
SKU: HY-N0930A
Rating: 4.5 (0 reviews)

Cat. No.: HY-N0930A Purity: 99.70%: Data Sheet
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Galegine hemisulfate, a guanidine derivative, contributes to weight loss in mice. Galegine hemisulfate activates AMPK in 3T3-L1 adipocytes and L6 myotubes, as well as in the H4IIE rat hepatoma and HEK293 human kidney cell lines. Galegine hemisulfate has antibacterial activity, with minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains.

For research use only. We do not sell to patients.

Galegine hemisulfate Chemical Structure

CAS No. : 14279-86-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Other Forms of Galegine hemisulfate:

Galegine hydrochloride Get quote

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1 BRSK1 BRSK2 HUNK AMPKα

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Pre-treatment with Galegine (10 μM-3 mM; 5 h) hemisulfate produces a concentration-dependent stimulation of insulin-independent glucose uptake by 3T3-L1 adipocytes without any effect on cell viability. Incubation with Galegine (1 μM-1 mM, 5 h) hemisulfate produces a concentration-dependent stimulation of glucose uptake into L6 myotubes, again without any effect on cell viability^[1].
Galegine (0.3-300 μM; 24 hours) hemisulfate produces a slight reduction in basal glycerol release and a more marked reduction in Isoprenaline (HY-108353)-stimulated glycerol release from 3T3-L1 adipocytes. Incubation of H4IIE rat hepatoma cells with Galegine (10 or 300 μM) hemisulfate for up to 6 hours produces a time-dependent activation of AMPK measured in cell lysates, with maximal activation at 360 min and twofold activation still evident at 24 hous in the presence of 300 μM Galegine hemisulfate. The effect of 300 ΜM Galegine hemisulfate is markedly greater than that of 10 μM. Incubation with Galegine hemisulfate for 1 hour produces a concentration-dependent activation of AMPK in both 3T3L-1 adipocytes and L-6 myotubes. Galegine hemisulfate also produces a concentration-dependent activation of the enzyme in a human kidney cell line (HEK293)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Galegine (63 mg/kg; feed; daily for 11 days) hemisulfate produces a significant reduction in body weight^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult male BALB/c mice^[1]
Dosage:	63 mg/kg
Administration:	feed; daily for 11 days
Result:	Produced a significant reduction in body weight.

Molecular Weight

176.23

Formula

C₆H₁₃N₃.1/2H₂O₄S

CAS No.

14279-86-8

Appearance

Solid

Color

White to off-white

SMILES

NC(NC/C=C(C)\C)=N.OS(O)(=O)=O.[1/2]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 40 mg/mL (226.98 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.6744 mL	28.3720 mL	56.7440 mL
5 mM	1.1349 mL	5.6744 mL	11.3488 mL
10 mM	0.5674 mL	2.8372 mL	5.6744 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.70%

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Data Sheet (275 KB) SDS (480 KB)

COA (250 KB) HNMR (198 KB) RP-HPLC (240 KB)

Handling Instructions (2659 KB)

References

[1]. Mooney MH, et al. Mechanisms underlying the metabolic actions of galegine that contribute to weight loss in mice. Br J Pharmacol. 2008 Apr;153(8):1669-77. [Content Brief]

[2]. Coqueiro A, et al. In Vitro Antibacterial Activity of Prenylated Guanidine Alkaloids from Pterogyne nitens and Synthetic Analogues. J Nat Prod. 2014 Aug 22;77(8):1972-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.6744 mL	28.3720 mL	56.7440 mL	141.8601 mL
	5 mM	1.1349 mL	5.6744 mL	11.3488 mL	28.3720 mL
	10 mM	0.5674 mL	2.8372 mL	5.6744 mL	14.1860 mL
	15 mM	0.3783 mL	1.8915 mL	3.7829 mL	9.4573 mL
	20 mM	0.2837 mL	1.4186 mL	2.8372 mL	7.0930 mL
	25 mM	0.2270 mL	1.1349 mL	2.2698 mL	5.6744 mL
	30 mM	0.1891 mL	0.9457 mL	1.8915 mL	4.7287 mL
	40 mM	0.1419 mL	0.7093 mL	1.4186 mL	3.5465 mL
	50 mM	0.1135 mL	0.5674 mL	1.1349 mL	2.8372 mL
	60 mM	0.0946 mL	0.4729 mL	0.9457 mL	2.3643 mL
	80 mM	0.0709 mL	0.3547 mL	0.7093 mL	1.7733 mL
	100 mM	0.0567 mL	0.2837 mL	0.5674 mL	1.4186 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.