Galegine hemisulfate
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Galegine hemisulfate, a guanidine derivative, contributes to weight loss in mice. Galegine hemisulfate activates AMPK in 3T3-L1 adipocytes and L6 myotubes, as well as in the H4IIE rat hepatoma and HEK293 human kidney cell lines. Galegine hemisulfate has antibacterial activity, with minimum inhibitory concentration of 4 mg/L against Staphylococcus aureus strains.
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- Pureté : 99.70%
- CAS No.: 14279-86-8
- Formule: C6H13N3.1/2H2O4S
- Masse moléculaire:176.23
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
Pre-treatment with Galegine (10 μM-3 mM; 5 h) hemisulfate produces a concentration-dependent stimulation of insulin-independent glucose uptake by 3T3-L1 adipocytes without any effect on cell viability. Incubation with Galegine (1 μM-1 mM, 5 h) hemisulfate produces a concentration-dependent stimulation of glucose uptake into L6 myotubes, again without any effect on cell viability[1].
Galegine (0.3-300 μM; 24 hours) hemisulfate produces a slight reduction in basal glycerol release and a more marked reduction in Isoprenaline (HY-108353)-stimulated glycerol release from 3T3-L1 adipocytes. Incubation of H4IIE rat hepatoma cells with Galegine (10 or 300 μM) hemisulfate for up to 6 hours produces a time-dependent activation of AMPK measured in cell lysates, with maximal activation at 360 min and twofold activation still evident at 24 hous in the presence of 300 μM Galegine hemisulfate. The effect of 300 ΜM Galegine hemisulfate is markedly greater than that of 10 μM. Incubation with Galegine hemisulfate for 1 hour produces a concentration-dependent activation of AMPK in both 3T3L-1 adipocytes and L-6 myotubes. Galegine hemisulfate also produces a concentration-dependent activation of the enzyme in a human kidney cell line (HEK293)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult male BALB/c mice[1]
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Dosage:63 mg/kg
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Administration:feed; daily for 11 days
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Result:Produced a significant reduction in body weight.
Chemical Information
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CAS No. 14279-86-8
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Appearance Solid
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Masse moléculaire 176.23
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Formule C6H13N3.1/2H2O4S
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Color White to off-white
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SMILES
NC(NC/C=C(C)\C)=N.OS(O)(=O)=O.[1/2]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
H2O : 40 mg/mL (226.98 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (275 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Mooney MH, et al. Mechanisms underlying the metabolic actions of galegine that contribute to weight loss in mice. Br J Pharmacol. 2008 Apr;153(8):1669-77. [Content Brief]
[2]. Coqueiro A, et al. In Vitro Antibacterial Activity of Prenylated Guanidine Alkaloids from Pterogyne nitens and Synthetic Analogues. J Nat Prod. 2014 Aug 22;77(8):1972-5. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 5.6744 mL | 28.3720 mL | 56.7440 mL | 141.8601 mL |
| 5 mM | 1.1349 mL | 5.6744 mL | 11.3488 mL | 28.3720 mL | |
| 10 mM | 0.5674 mL | 2.8372 mL | 5.6744 mL | 14.1860 mL | |
| 15 mM | 0.3783 mL | 1.8915 mL | 3.7829 mL | 9.4573 mL | |
| 20 mM | 0.2837 mL | 1.4186 mL | 2.8372 mL | 7.0930 mL | |
| 25 mM | 0.2270 mL | 1.1349 mL | 2.2698 mL | 5.6744 mL | |
| 30 mM | 0.1891 mL | 0.9457 mL | 1.8915 mL | 4.7287 mL | |
| 40 mM | 0.1419 mL | 0.7093 mL | 1.4186 mL | 3.5465 mL | |
| 50 mM | 0.1135 mL | 0.5674 mL | 1.1349 mL | 2.8372 mL | |
| 60 mM | 0.0946 mL | 0.4729 mL | 0.9457 mL | 2.3643 mL | |
| 80 mM | 0.0709 mL | 0.3547 mL | 0.7093 mL | 1.7733 mL | |
| 100 mM | 0.0567 mL | 0.2837 mL | 0.5674 mL | 1.4186 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Galegine hemisulfate Related Classifications
HY-N0930A Related Classifications
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