Memantine
Based on 5 publication(s) in Google Scholar
Memantine is an orally active, noncompetitive N-methyl-D-aspartate receptor (NMDAR) antagonist. Memantine can be used for the research of moderate-to-severe Alzheimer's disease (AD).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.89%
- No. CAS: 19982-08-2
- Fòrmula: C12H21N
- Peso molecular:179.30
-
Almacenamiento:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Memantine
MoreVer todos los productos específicos de isoformas iGluR
More
Actividad biológica
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| Cerebellar granule neurone | EC50 |
2.48 μM
Compound: Memantine
|
Neuroprotective activity against glutamate-induced excitotoxicity in mouse primary cerebellar granule neurons assessed as increase in cell viability preincubated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay
Neuroprotective activity against glutamate-induced excitotoxicity in mouse primary cerebellar granule neurons assessed as increase in cell viability preincubated for 2 hrs followed by glutamate challenge measured after 24 hrs by MTT assay
|
[PMID: 30108699] |
| FM3A | IC50 |
147 μM
Compound: Memantine
|
Cytotoxicity in mouse FM3A cells after 48 hrs
Cytotoxicity in mouse FM3A cells after 48 hrs
|
[PMID: 17624774] |
| HEK293 | IC50 |
27.2 μM
Compound: memantine
|
Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy
Inhibition of 4-(4-(dimethylamino)styryl)-N-methylpyridinium uptake at human OCT1 expressed in HEK293 cells by confocal microscopy
|
[PMID: 18788725] |
| Oocyte | IC50 |
0.95 nM
Compound: Memantine
|
Antagonist activity at recombinant GluN1/GluN2A receptor (unknown origin) expressed in Xenopus laevis oocytes assessed as inhibition of glycine/glutamate-induced inward current at -70mV by two-electrode voltage clamp assay
Antagonist activity at recombinant GluN1/GluN2A receptor (unknown origin) expressed in Xenopus laevis oocytes assessed as inhibition of glycine/glutamate-induced inward current at -70mV by two-electrode voltage clamp assay
|
[PMID: 23692871] |
| Oocyte | IC50 |
9.52 μM
Compound: 1, memantine
|
Inhibition of NMDA NR1/NR2B receptor expressed in xenopus oocytes assessed as inhibition of NMDA and glycine-induced current response by two-electrode voltage clamp assay
Inhibition of NMDA NR1/NR2B receptor expressed in xenopus oocytes assessed as inhibition of NMDA and glycine-induced current response by two-electrode voltage clamp assay
|
[PMID: 18605718] |
| SH-SY5Y | GI50 |
145 μM
Compound: 4
|
Neurotoxicity against human SH-SY5Y cells assessed as growth inhibition measured after 48 hrs by MTT assay
Neurotoxicity against human SH-SY5Y cells assessed as growth inhibition measured after 48 hrs by MTT assay
|
[PMID: 27688184] |
| SH-SY5Y | IC50 |
718 nM
Compound: 2
|
Neuroprotective activity in human SH-SY5Y cells assessed as protection against NMDA-induced cell death after 6 hrs by MTS assay
Neuroprotective activity in human SH-SY5Y cells assessed as protection against NMDA-induced cell death after 6 hrs by MTS assay
|
[PMID: 23033965] |
Memantine (1-3 mg/kg; per os (p.o.)) for 14 days significantly improves depressive-like behaviors in olfactory bulbectomized (OBX) mice, as assessed using the tail-suspension and forced-swim tests[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Male Wistar rats[2]
-
Dosage:1, 5 and 10, 15 mg/kg
-
Administration:i.p.; daily for 15-20 days
-
Result:Significantly decreased the large LTP recorded in hippocampal slices from aged animals.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
-
No. CAS 19982-08-2
-
Appearance Solid
-
Peso molecular 179.30
-
Fòrmula C12H21N
-
Color White to light yellow
-
SMILES
N[C@]12C[C@@]3(C)C[C@](C2)(C)C[C@@H](C1)C3
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
-
Journal Impact Factor
-
Most Recent
-
-
Int J Mol Sci
2024 Nov 19;25(22):12404. PMID: 39596468 -
Biomedicines
Alpha7 Nicotinic Acetylcholine Receptor Antagonists Prevent Meningitic Escherichia coli-Induced Blood-Brain Barrier Disruptions by Targeting the CISH/JAK2/STAT5b Axis. [Abstract]2022 Sep 22;10(10):2358. PMID: 36289622 -
Pharmacol Res Perspect
Effects of membrane transport activity and cell metabolism on the unbound drug concentrations in the skeletal muscle and liver of drugs: A microdialysis study in rats. [Abstract]2021 Oct;9(5):e00879. PMID: 34628723 -
Solvente y solubilidad
DMSO : 10 mg/mL (55.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (5.58 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (5.58 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (277 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
[1]. Marotta G, et al. Memantine Derivatives as Multitarget Agents in Alzheimer's Disease. Molecules. 2020;25(17):4005. Published 2020 Sep 2. [Content Brief]
[2]. Pinho J, et al. Enhanced LTP in aged rats: Detrimental or compensatory?. Neuropharmacology. 2017;114:12-19. [Content Brief]
[3]. Moriguchi S, et al. Memantine Improves Depressive-like Behaviors via Kir6.1 Channel Inhibition in Olfactory Bulbectomized Mice. Neuroscience. 2020;442:264-273. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.5772 mL | 27.8862 mL | 55.7724 mL | 139.4311 mL |
| 5 mM | 1.1154 mL | 5.5772 mL | 11.1545 mL | 27.8862 mL | |
| 10 mM | 0.5577 mL | 2.7886 mL | 5.5772 mL | 13.9431 mL | |
| 15 mM | 0.3718 mL | 1.8591 mL | 3.7182 mL | 9.2954 mL | |
| 20 mM | 0.2789 mL | 1.3943 mL | 2.7886 mL | 6.9716 mL | |
| 25 mM | 0.2231 mL | 1.1154 mL | 2.2309 mL | 5.5772 mL | |
| 30 mM | 0.1859 mL | 0.9295 mL | 1.8591 mL | 4.6477 mL | |
| 40 mM | 0.1394 mL | 0.6972 mL | 1.3943 mL | 3.4858 mL | |
| 50 mM | 0.1115 mL | 0.5577 mL | 1.1154 mL | 2.7886 mL |
Memantine Related Classifications
HY-B0591 Related Classifications
Powered by Bioz