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Referencia número: HY-Q36691

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PRMT5-IN-30

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PRMT5-IN-30 (compound 17) is a potent and selective protein arginine methyltransferase 5 (PRMT5) inhibitor with an IC₅₀ of 0.33 μM and a K_d of 0.987 μM. PRMT5-IN-30 exhibits a broad selectivity against a panel of other methyltransferases. PRMT5-IN-30 inhibits PRMT5 mediated SmD3 methylation.

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Pureza: 99.35%
No. CAS: 330951-01-4
Fòrmula: C₁₈H₁₇N₃O₄S
Peso molecular:371.41

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

PRMT5-IN-30 Chemical Structure

No. CAS: 330951-01-4

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
Chemical Information
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target^[1]

PRMT5

0.33 μM (IC₅₀)

PRMT5

0.987 μM (Kd)

Ver todos los productos específicos de isoformas Histone Methyltransferase :

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	>100 μM Compound: 17	Inhibition of recombinant human N-terminal FLAG-tagged PRMT4 (2 to end residues) expressed in HEK293F cells using of histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA Inhibition of recombinant human N-terminal FLAG-tagged PRMT4 (2 to end residues) expressed in HEK293F cells using of histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl methionine addition measured after 60 mins by alpha LISA	[PMID: 28650658]
HEK293	IC₅₀	0.33 μM Compound: 17	Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl meth Inhibition of recombinant human N-terminal FLAG-tagged PRMT5 (2 to end residues) /human N-terminal His-tagged MEP50 (2 to end residues) expressed in HEK293F cells using histone H4 as substrate preincubated for 15 mins followed by substrate/S-adenosyl meth	[PMID: 28650658]
JeKo-1	EC₅₀	15 μM Compound: 17	Cytotoxicity against human JeKo1 cells assessed as cell growth inhibition after 12 days by Cell Titer-Glo assay Cytotoxicity against human JeKo1 cells assessed as cell growth inhibition after 12 days by Cell Titer-Glo assay	[PMID: 28650658]
KOPN-8	EC₅₀	15 μM Compound: 17	Cytotoxicity against human KOPN8 cells assessed as cell growth inhibition after 8 days by Cell Titer-Glo assay Cytotoxicity against human KOPN8 cells assessed as cell growth inhibition after 8 days by Cell Titer-Glo assay	[PMID: 28650658]
MV4-11	EC₅₀	25.73 μM Compound: 17	Cytotoxicity against human MV411 cells assessed as cell growth inhibition after 8 days by Cell Titer-Glo assay Cytotoxicity against human MV411 cells assessed as cell growth inhibition after 8 days by Cell Titer-Glo assay	[PMID: 28650658]
MV4-11	EC₅₀	6.53 μM Compound: 17	Cytotoxicity against human MV411 cells assessed as cell growth inhibition after 12 days by Cell Titer-Glo assay Cytotoxicity against human MV411 cells assessed as cell growth inhibition after 12 days by Cell Titer-Glo assay	[PMID: 28650658]
MV4-11	EC₅₀	80.74 μM Compound: 17	Cytotoxicity against human MV411 cells assessed as cell growth inhibition after 4 days by Cell Titer-Glo assay Cytotoxicity against human MV411 cells assessed as cell growth inhibition after 4 days by Cell Titer-Glo assay	[PMID: 28650658]
Z-138	EC₅₀	15 μM Compound: 17	Cytotoxicity against human Z138 cells assessed as cell growth inhibition after 12 days by Cell Titer-Glo assay Cytotoxicity against human Z138 cells assessed as cell growth inhibition after 12 days by Cell Titer-Glo assay	[PMID: 28650658]

Chemical Information

No. CAS 330951-01-4
Appearance Solid
Peso molecular 371.41
Fòrmula C₁₈H₁₇N₃O₄S
Color White to off-white
SMILES

C1(SCC(=O)NC2C=CC=CC=2C(OC)=O)NC2C(=CC=C(OC)C=2)N=1
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 50 mg/mL (134.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6924 mL

13.4622 mL

26.9244 mL

5 mM

0.5385 mL

2.6924 mL

5.3849 mL

10 mM

0.2692 mL

1.3462 mL

2.6924 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Ruifeng Mao, et al. Potent, Selective, and Cell Active Protein Arginine Methyltransferase 5 (PRMT5) Inhibitor Developed by Structure-Based Virtual Screening and Hit Optimization. J Med Chem. 2017 Jul 27;60(14):6289-6304. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6924 mL	13.4622 mL	26.9244 mL	67.3111 mL
	5 mM	0.5385 mL	2.6924 mL	5.3849 mL	13.4622 mL
	10 mM	0.2692 mL	1.3462 mL	2.6924 mL	6.7311 mL
	15 mM	0.1795 mL	0.8975 mL	1.7950 mL	4.4874 mL
	20 mM	0.1346 mL	0.6731 mL	1.3462 mL	3.3656 mL
	25 mM	0.1077 mL	0.5385 mL	1.0770 mL	2.6924 mL
	30 mM	0.0897 mL	0.4487 mL	0.8975 mL	2.2437 mL
	40 mM	0.0673 mL	0.3366 mL	0.6731 mL	1.6828 mL
	50 mM	0.0538 mL	0.2692 mL	0.5385 mL	1.3462 mL
	60 mM	0.0449 mL	0.2244 mL	0.4487 mL	1.1219 mL
	80 mM	0.0337 mL	0.1683 mL	0.3366 mL	0.8414 mL
	100 mM	0.0269 mL	0.1346 mL	0.2692 mL	0.6731 mL

Keywords

100 mg

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Actividad biológica
- Descripciòn
- IC₅₀ & Target
Chemical Information
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias

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Nombre del producto:
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PRMT5-IN-30

Ver todos los productos específicos de isoformas Histone Methyltransferase

Actividad biológica

Chemical Information

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

PRMT5-IN-30 Related Classifications

HY-Q36691 Related Classifications