2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. GC-7

GC7 Sulfate is a deoxyhypusine synthase (DHPS) inhibitor.

For research use only. We do not sell to patients.

CAS No. : 150333-69-0

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of GC-7:

Other Forms of GC-7:

GC-7 Related Antibodies

Top Publications Citing Use of Products

    GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113.

    PC3 and DU145 cells were pretreated with GC7 (2 μmol/L) for 6 hours, then cultured for another 24 hours with the specified reagent, and cell viability was then assessed.

    GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113.

    Western blot analysis of Hyp-EIF5A, EIF5A, NRF2, and HMOX1 in PC3 cells. Endogenous polyamines in PC3 cells were depleted by incubation with 1 mmol/L DFMO for 2 days. Polyamine-depleted cells were pretreated with GC7 (2 μmol/L) for 6 hours, followed by treatment with Spd and erastin for 8 hours.

    GC-7 purchased from MedChemExpress. Usage Cited in: Acta Pharm Sin B. 2025 Apr;15(4):2095-2113.

    GC 7 (2 mg/kg, intraperitoneal injection). The volume of the PC3 xenograft tumor was monitored every other day, and the tumor weight was divided into 4 groups.

    GC-7 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Mar 19;15(1):2461.  [Abstract]

    Cell viability in A549 or HT1080 cells treated with RSL3 following pre-treatment with 1 μM GC7 for 16 h and polyamines.

    GC-7 purchased from MedChemExpress. Usage Cited in: Research Square Preprint. 2021 Nov.

    Experimental results show that GC7 inhibits hypoxanthine modification of eIF5A in a concentration-dependent manner.

    View All DNA/RNA Synthesis Isoform Specific Products:

    View All Isoforms
    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    GC7 Sulfate is a deoxyhypusine synthase (DHPS) inhibitor.

    IC50 & Target

    DHPS[1]
    Cellular Effect
    Cell Line Type Value Description References
    A-375 IC50
    1.48 μM
    Compound: GC7
    Cytotoxicity against human A-375 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    Cytotoxicity against human A-375 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    		[PMID: 34473510]
    B16 IC50
    5.25 μM
    Compound: GC7
    Cytotoxicity against mouse B16 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    Cytotoxicity against mouse B16 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    		[PMID: 34473510]
    HaCaT CC50
    3.96 μM
    Compound: GC7
    Cytotoxicity against human HaCaT cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    Cytotoxicity against human HaCaT cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    		[PMID: 34473510]
    SK-MEL-28 IC50
    0.79 μM
    Compound: GC7
    Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    Cytotoxicity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by CCK-8 assay
    		[PMID: 34473510]
    In Vitro

    The treatment of MYCN2 (±Dox) and BE(2)-C cells with GC7 for 72 h at various concentrations (0.1 to 100 μM) significantly reduces the number of viable cells in a dose-dependent manner. In MYCN2 cells, 5 μM of GC7 inhibits cell viability by ~40 and ~60 %, respectively, compare to untreated control cells. BE(2)-C cells require 25 μM of GC7 to reduce cell viability by ~50 %. Exposure to 10 and 100 μM GC7 for 72 h clearly decreases the levels of total retinoblastoma (Rb) and phosphorylated Rb as well as of Cdk4 protein, and increases the levels of p21 protein[1]. Between 0 and 20 μM, GC7 induces little cytotoxicity in HCC cells, while higher concentrations of GC7 (50 to 100 μM) significantly inhibit the viability of all five HCC cell lines tested. Newly synthesized 3H-labeled hypusine of eIF5A1/eIF5A2 is rarely detected after 20 μM GC7 treatment, compare to untreated control. The activity of [3H]-spermidine incorporated into HCC cells is significantly decreased by 20 μM GC7 or higher concentration[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    GC-7 Related Antibodies
    Clinical Trial
    Molecular Weight

    172.27

    Formula

    C8H20N4
    CAS No.
    SMILES

    NC(NCCCCCCCN)=N
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
    Cell Assay
    [1]

    Cell viability is analyzed with the MTS assay kit in accordance with the manufacturer’s protocols. Briefly, after treatment with GC7 for 72 h at various concentrations (0.1 to 100 μM) in 96-well plates, cells are directly incubated with the MTS dye reagent for 1 h at 37°C in a 5 % CO2 atmosphere. The absorbance is read at 490 nm using a reader[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    GC-7 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    GC-7150333-69-0GC7GC 7DNA/RNA SynthesisInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    GC-7
    Cat. No.:
    HY-108314
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.