1. Stem Cell/Wnt
  2. Wnt
  3. iCRT 14

iCRT 14 

Cat. No.: HY-16665 Purity: 98.90%
Handling Instructions

iCRT 14 is a novel potent inhibitor of β-catenin-responsive transcription (CRT), with IC50 of 40.3 nM against Wnt responsive STF16 luciferase.

For research use only. We do not sell to patients.

iCRT 14 Chemical Structure

iCRT 14 Chemical Structure

CAS No. : 677331-12-3

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10 mM * 1 mL in DMSO USD 92 In-stock
Estimated Time of Arrival: December 31
5 mg USD 84 In-stock
Estimated Time of Arrival: December 31
10 mg USD 144 In-stock
Estimated Time of Arrival: December 31
50 mg USD 624 In-stock
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE iCRT 14

    iCRT 14 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Apr 1;9(4):433.

    rECA109 and rKyse150 cells show preferential activation of DNA damage checkpoint responses upon exposure to ionizing radiation (IR) relative to parental cells. And WNT1 promoted preferential activation of DNA damage response while iCRT14 hinders it.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    iCRT 14 is a novel potent inhibitor of β-catenin-responsive transcription (CRT), with IC50 of 40.3 nM against Wnt responsive STF16 luciferase.

    IC50 & Target

    IC50: 40.3 nM (Wnt responsive STF16 luciferase)[1]

    In Vitro

    iCRT14 can interfere with TCF binding to DNA in addition to its ability to influence TCF-β-cat interaction[1]. iCRT14 (10, 25, 50 μM) effectively inhibits cell proliferation in BT-549 cells in a dose- and time-dependent manner, but still less potent than iCRT3[2].

    In Vivo

    iCRT14 (50 mg/kg, i.p.) markedly decreases CycD1, proliferation of the tumors in HCT116 xenografts[1].

    Molecular Weight

    375.44

    Formula

    C₂₁H₁₇N₃O₂S

    CAS No.

    677331-12-3

    SMILES

    O=C(S/1)N(C2=CC=CC=C2)C(C1=C\C3=C(C)N(C(C)=C3)C4=CN=CC=C4)=O

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 29 mg/mL (77.24 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.6635 mL 13.3177 mL 26.6354 mL
    5 mM 0.5327 mL 2.6635 mL 5.3271 mL
    10 mM 0.2664 mL 1.3318 mL 2.6635 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [2]

    Cells are seeded at 20,000 cells/well into 96-well plates. After overnight incubation, cells are treated with DMSO or each Wnt inhibitor (iCRT-3, 75 μM; iCRT-5, 200 μM; iCRT-14, 50 μM; IWP-4, 5 μM and XAV-939, 10 μM) for 48 hours. Cell viability is determined using the Cell Titer-Glo luminescent cell viability assay kit. Luminescence is measured using FLUOstar microplate reader. All treatments are performed in triplicate, and each experiment is repeated three times.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 98.90%

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    Product Name:
    iCRT 14
    Cat. No.:
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