1. Cell Cycle/DNA Damage
    Cytoskeleton
    Apoptosis
  2. Microtubule/Tubulin
    Apoptosis
  3. Indibulin

Indibulin (Synonyms: ZIO 301; D 24851)

Cat. No.: HY-13649
Handling Instructions

Indibulin (ZIO 301), an orally applicable inhibitor of tubulin assembly, shows potent anticancer activity with a minimal neurotoxicity. Indibulin reduces inter-kinetochoric tension, produces aberrant spindles, activates mitotic checkpoint proteins Mad2 and BubR1, and induces mitotic arrest and apoptosis.

For research use only. We do not sell to patients.

Indibulin Chemical Structure

Indibulin Chemical Structure

CAS No. : 204205-90-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 121 In-stock
Estimated Time of Arrival: December 31
5 mg USD 110 In-stock
Estimated Time of Arrival: December 31
10 mg USD 160 In-stock
Estimated Time of Arrival: December 31
50 mg USD 580 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1020 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Indibulin (ZIO 301), an orally applicable inhibitor of tubulin assembly, shows potent anticancer activity with a minimal neurotoxicity. Indibulin reduces inter-kinetochoric tension, produces aberrant spindles, activates mitotic checkpoint proteins Mad2 and BubR1, and induces mitotic arrest and apoptosis[1].

IC50 & Target

Tubulin[1]

In Vitro

Indibulin (300-2100 nM; 48 hours) inhibits the proliferation of MCF-7 cells with an IC50 of 150 nM[1].
Indibulin (300, 600 nM; 48 hours) blockes the cells in the G2/M phase indicating that indibulin blockes the progression of the cell cycle at mitosis[1].
Indibulin (150-600 nM; 24 hours) induces apoptosis in MCF-7 cells[1].
Indibulin (150-600 nM; 48 hours) with 300 and 600 nM generates cleaved fragments of PARP protein the treatment of MCF-7 cells[1].

Cell Proliferation Assay[1]

Cell Line: MCF-7 cells
Concentration: 300, 600, 900, 1200, 1500, 1800, 2100 nM
Incubation Time: 48 hours
Result: Inhibited the proliferation of MCF-7 cells with an IC50 of 150 nM.

Cell Cycle Analysis[1]

Cell Line: MCF-7 cells
Concentration: 300, 600 nM
Incubation Time: 48 hours
Result: Blocked the cells in the G2/M phase of the cell cycle.

Apoptosis Analysis[1]

Cell Line: MCF-7 cells
Concentration: 150, 300 and 600 nM
Incubation Time: 24 hours
Result: Induced apoptosis in MCF-7 cells.

Western Blot Analysis[1]

Cell Line: MCF-7 cells
Concentration: 150, 300 and 600 nM
Incubation Time: 48 hours
Result: Generated cleaved fragments of PARP protein in 300 and 600 nM.
Molecular Weight

389.83

Formula

C₂₂H₁₆ClN₃O₂

CAS No.

204205-90-3

SMILES

O=C(NC1=CC=NC=C1)C(C2=CN(CC3=CC=C(Cl)C=C3)C4=C2C=CC=C4)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (128.26 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5652 mL 12.8261 mL 25.6522 mL
5 mM 0.5130 mL 2.5652 mL 5.1304 mL
10 mM 0.2565 mL 1.2826 mL 2.5652 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.08 mg/mL (5.34 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.34 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

IndibulinZIO 301D 24851ZIO301ZIO-301D24851D-24851Microtubule/TubulinApoptosisInhibitorinhibitorinhibit

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Indibulin
Cat. No.:
HY-13649
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