Isodecyl diphenyl phosphate
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Isodecyl diphenyl phosphate (IDPP) is a flame retardant. Isodecyl diphenyl phosphate induces cytotoxicity, DNA damage, mitochondrial dysfunction and oxidative stress in cancer cells, and mediates DNA damage and cell cycle arrest via a p53-dependent pathway.
For research use only. We do not sell to patients.
- Purity : 99.40%
- CAS No.: 29761-21-5
- Formula: C22H31O4P
- Molecular Weight:390.45
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Storage:
4°C, stored under argon
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under argon)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Isodecyl diphenyl phosphate (0.25-1024 μM; 96 h) induces concentration-dependent cytotoxicity in A549 cells[1].
Isodecyl diphenyl phosphate (0-100 μM; 96 h) induces a concentration-dependent increase in DNA content in A549 cells after 96 h of exposure, suggesting its potential DNA-damaging effect[1].
Isodecyl diphenyl phosphate (2.5-50 μM; 96 h) induces concentration-dependent S-phase cell cycle arrest in A549 cells[1].
Isodecyl diphenyl phosphate (1.02-100 μM; 96 h) induces concentration-dependent mitochondrial damage in A549 cells after 96 h of exposure, characterized by increased mitochondrial mass and decreased mitochondrial membrane potential[1].
Isodecyl diphenyl phosphate (1.02-100 μM) induces concentration-dependent activation of the p53 pathway in p53-transfected A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549
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Concentration:0.25-1024 μM
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Incubation Time:96 h
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Result:Induced concentration-dependent cytotoxicity in A549 cells, with a calculated lethal concentration 50 (LC50) value of 130.45 μM.
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Cell Line:A549
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Concentration:2.5, 25, 50 μM
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Incubation Time:96 h
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Result:Induced a concentration-dependent decrease in the S phase cell ratio in A549 cells, with a significant maximal reduction of 73.5% relative to control observed at the 50 μM concentration.
Chemical Information
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CAS No. 29761-21-5
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Appearance Liquid (Density: 1.07 g/cm3)
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Molecular Weight 390.45
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Formula C22H31O4P
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Color Colorless to light yellow
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SMILES
O=P(OC1=CC=CC=C1)(OCCCCCCCC(C)C)OC2=CC=CC=C2
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Synonyms
IDPP
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under argon
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under argon)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (256.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under argon). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under argon). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.40 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under argon)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under argon). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5611 mL | 12.8057 mL | 25.6115 mL | 64.0287 mL |
| 5 mM | 0.5122 mL | 2.5611 mL | 5.1223 mL | 12.8057 mL | |
| 10 mM | 0.2561 mL | 1.2806 mL | 2.5611 mL | 6.4029 mL | |
| 15 mM | 0.1707 mL | 0.8537 mL | 1.7074 mL | 4.2686 mL | |
| 20 mM | 0.1281 mL | 0.6403 mL | 1.2806 mL | 3.2014 mL | |
| 25 mM | 0.1024 mL | 0.5122 mL | 1.0245 mL | 2.5611 mL | |
| 30 mM | 0.0854 mL | 0.4269 mL | 0.8537 mL | 2.1343 mL | |
| 40 mM | 0.0640 mL | 0.3201 mL | 0.6403 mL | 1.6007 mL | |
| 50 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2806 mL | |
| 60 mM | 0.0427 mL | 0.2134 mL | 0.4269 mL | 1.0671 mL | |
| 80 mM | 0.0320 mL | 0.1601 mL | 0.3201 mL | 0.8004 mL | |
| 100 mM | 0.0256 mL | 0.1281 mL | 0.2561 mL | 0.6403 mL |
Isodecyl diphenyl phosphate Related Classifications
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