Isodecyl diphenyl phosphate (Synonyms: IDPP)

Cat. No.: HY-W718423 Purity: 99.40%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Isodecyl diphenyl phosphate (IDPP) is a flame retardant. Isodecyl diphenyl phosphate induces cytotoxicity, DNA damage, mitochondrial dysfunction and oxidative stress in cancer cells, and mediates DNA damage and cell cycle arrest via a p53-dependent pathway.

For research use only. We do not sell to patients.

Isodecyl diphenyl phosphate Chemical Structure

CAS No. : 29761-21-5

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Isodecyl diphenyl phosphate (0.25-1024 μM; 96 h) induces concentration-dependent cytotoxicity in A549 cells^[1].
Isodecyl diphenyl phosphate (0-100 μM; 96 h) induces a concentration-dependent increase in DNA content in A549 cells after 96 h of exposure, suggesting its potential DNA-damaging effect^[1].
Isodecyl diphenyl phosphate (2.5-50 μM; 96 h) induces concentration-dependent S-phase cell cycle arrest in A549 cells^[1].
Isodecyl diphenyl phosphate (1.02-100 μM; 96 h) induces concentration-dependent mitochondrial damage in A549 cells after 96 h of exposure, characterized by increased mitochondrial mass and decreased mitochondrial membrane potential^[1].
Isodecyl diphenyl phosphate (1.02-100 μM) induces concentration-dependent activation of the p53 pathway in p53-transfected A549 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	A549
Concentration:	0.25-1024 μM
Incubation Time:	96 h
Result:	Induced concentration-dependent cytotoxicity in A549 cells, with a calculated lethal concentration 50 (LC₅₀) value of 130.45 μM.

Cell Cycle Analysis^[1]

Cell Line:	A549
Concentration:	2.5, 25, 50 μM
Incubation Time:	96 h
Result:	Induced a concentration-dependent decrease in the S phase cell ratio in A549 cells, with a significant maximal reduction of 73.5% relative to control observed at the 50 μM concentration.

Molecular Weight

390.45

Formula

C₂₂H₃₁O₄P

CAS No.

29761-21-5

Appearance

Liquid (Density: 1.07 g/cm³)

Color

Colorless to light yellow

SMILES

O=P(OC1=CC=CC=C1)(OCCCCCCCC(C)C)OC2=CC=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (256.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5611 mL	12.8057 mL	25.6115 mL
5 mM	0.5122 mL	2.5611 mL	5.1223 mL
10 mM	0.2561 mL	1.2806 mL	2.5611 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.40 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (271 KB) SDS (254 KB)

COA (244 KB) HNMR (178 KB) RP-HPLC (239 KB) MS (242 KB)

Handling Instructions (2659 KB)

References

[1]. Yuan S, et al. Aryl-phosphorus-containing flame retardants induce oxidative stress, the p53-dependent DNA damage response and mitochondrial impairment in A549 cells. Environ Pollut. 2019 Jul;250:58-67. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5611 mL	12.8057 mL	25.6115 mL	64.0287 mL
	5 mM	0.5122 mL	2.5611 mL	5.1223 mL	12.8057 mL
	10 mM	0.2561 mL	1.2806 mL	2.5611 mL	6.4029 mL
	15 mM	0.1707 mL	0.8537 mL	1.7074 mL	4.2686 mL
	20 mM	0.1281 mL	0.6403 mL	1.2806 mL	3.2014 mL
	25 mM	0.1024 mL	0.5122 mL	1.0245 mL	2.5611 mL
	30 mM	0.0854 mL	0.4269 mL	0.8537 mL	2.1343 mL
	40 mM	0.0640 mL	0.3201 mL	0.6403 mL	1.6007 mL
	50 mM	0.0512 mL	0.2561 mL	0.5122 mL	1.2806 mL
	60 mM	0.0427 mL	0.2134 mL	0.4269 mL	1.0671 mL
	80 mM	0.0320 mL	0.1601 mL	0.3201 mL	0.8004 mL
	100 mM	0.0256 mL	0.1281 mL	0.2561 mL	0.6403 mL

Isodecyl diphenyl phosphate Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isodecyl diphenyl29761-21-5IDPPDNA/RNA SynthesisMDM-2/p53A549 cellsapoptosis pathwayp53/baxlung carcinomaDNA damagep53p53/mdm2cell cycle arrestmitochondrial impairmentInhibitorinhibitorinhibit

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