NSC-87877
Based on 3 publication(s) in Google Scholar
NSC-87877 is a potent inhibitor of Shp2 and Shp1 protein tyrosine phosphatases (SH-PTP2 and SH-PTP1), with IC50 values of 0.318 μM, 0.355 μM shp2 and shp1, respectively. NSC-87877 also inhibits dual-specificity phosphatase 26 (DUSP26).
For research use only. We do not sell to patients.
- Purity: 98.33%
- CAS No.: 56990-57-9
- Formula: C19H13N3O7S2
- Molecular Weight:459.45
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
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Kinase Inhibitor Library
HY-L009
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Metabolism/Protease Compound Library
HY-L012
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Protein Tyrosine Kinase Compound Library
HY-L016
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Oxygen Sensing Compound Library
HY-L045
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Phosphatase Inhibitor Library
HY-L081
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HY-L146
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Publications Citing Use of MedChemExpress (MCE) NSC-87877
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Others
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RT-PCR
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IF
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WB
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Histological Imaging/Staining
Biological Activity
IC50: 0.318 μM (shp2), 0.355 μM (shp1)[1].
NSC-87877 (0-0.5 μM, 5 days) inhibits DUSP26 function in NB cell lines[3].
NSC-87877 (0-0.5 μM, 5 days) results in increased p53 phosphorylation (Ser37 and Ser46) and activation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:p53 wild-type neuroblastoma (NB) cell lines[2].
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Concentration:0, 0.25, 0.5 μM.
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Incubation Time:5 dsys.
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Result:Resulted in increased p53 phosphorylation (Ser37 and Ser46) and activation, increased activation of downstream p38 effector proteins (heat shock protein 27 (HSP27) and MAP kinase-activated protein kinase 2 (MAPKAPK2)) and poly ADP ribose polymerase/caspase-3 cleavage.
Inhibited DUSP26 function in NB cell lines.
Resulted in apoptosis in many cell lines at varying IC50 levels of 1.84 μM (IMR32), 6.35 μM (SK-N-SH), 8.69 μM (NB-19), 12.6 μM (SMS-KCN), 15.7 μM (SH-SY5Y), 15.8 μM (JF) and 19.0 μM (CHLA-225), respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Intrarenal neuroblastoma (NB) tumor mouse model in female nude mice[3].
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Dosage:30 mg/kg.
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Administration:IP once daily for 15 days.
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Result:Significantly inhibited NB tumor growth.
Chemical Information
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CAS No. 56990-57-9
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Appearance Solid
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Molecular Weight 459.45
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Formula C19H13N3O7S2
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Color Brown to black
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SMILES
O=S(C1=C2C=CC=NC2=C(O)C(/N=N/C3=CC=C4C=C(S(=O)(O)=O)C=CC4=C3)=C1)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Immunity
B cell-derived acetylcholine promotes liver regeneration by regulating Kupffer cell and hepatic CD8+ T cell function. [Abstract]2025 Apr 23:S1074-7613(25)00165-7. PMID: 40286791 -
Sci Transl Med
Hematopoietic loss of Y chromosome activates immune checkpoints and contributes to impaired senescent cell clearance and renal disease. [Abstract]2025 Aug 6;17(810):eadv4071. PMID: 40768598
NSC-87877 purchased from MedChemExpress. Usage Cited in: Sci Transl Med. 2025 Aug 6;17(810):eadv4071. [Abstract]
The percentage of macrophages engulfing senescent cells with or without NSC-87877 (30 mg/kg).
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Neural Regen Res
Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model. [Abstract]2025 Mar 1;20(3):858-872. PMID: 38886958
NSC-87877 purchased from MedChemExpress. Usage Cited in: Neural Regen Res. 2025 Mar 1;20(3):858-872. [Abstract]
The mRNA expression levels of SHP2, iNOS, CD86, TNF-α, IL-1β, CD206 and Arg-1 in microglia treated with NSC-87877 and siSHP2 were detected by quantitative polymerase chain reaction (qPCR).
NSC-87877 purchased from MedChemExpress. Usage Cited in: Neural Regen Res. 2025 Mar 1;20(3):858-872. [Abstract]
Immunofluorescence staining was used to detect the immunoreactivity of CD163 (green - CoraLite488) and CD86 (red - CoraLite594) in microglia treated with NSC-87877.
NSC-87877 purchased from MedChemExpress. Usage Cited in: Neural Regen Res. 2025 Mar 1;20(3):858-872. [Abstract]
The expression of SHP2, iNOS, CD86, CD206, Arg-1 and other proteins in microglia treated with NSC-87877 was detected by Western blotting.
NSC-87877 purchased from MedChemExpress. Usage Cited in: Neural Regen Res. 2025 Mar 1;20(3):858-872. [Abstract]
HE staining of mouse spinal cord sections 14 days after SCI. HE staining of transverse and longitudinal sections showed that the lesion area in the SCI+NSC87877 (2 mM) group was significantly smaller than that in the SCI group.
Solvent & Solubility
DMSO : 25 mg/mL (54.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen L, et al. Discovery of a novel shp2 protein tyrosine phosphatase inhibitor. Mol Pharmacol. 2006 Aug;70(2):562-70. [Content Brief]
[2]. Song M, et al. NSC-87877, inhibitor of SHP-1/2 PTPs, inhibits dual-specificity phosphatase 26 (DUSP26). Biochem Biophys Res Commun. 2009 Apr 17;381(4):491-5. [Content Brief]
[3]. Y Shi, et al. NSC-87877 inhibits DUSP26 function in neuroblastoma resulting in p53-mediated apoptosis. Cell Death Dis. 2015 Aug 6;6(8):e1841. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.1765 mL | 10.8826 mL | 21.7652 mL | 54.4129 mL |
| 5 mM | 0.4353 mL | 2.1765 mL | 4.3530 mL | 10.8826 mL | |
| 10 mM | 0.2177 mL | 1.0883 mL | 2.1765 mL | 5.4413 mL | |
| 15 mM | 0.1451 mL | 0.7255 mL | 1.4510 mL | 3.6275 mL | |
| 20 mM | 0.1088 mL | 0.5441 mL | 1.0883 mL | 2.7206 mL | |
| 25 mM | 0.0871 mL | 0.4353 mL | 0.8706 mL | 2.1765 mL | |
| 30 mM | 0.0726 mL | 0.3628 mL | 0.7255 mL | 1.8138 mL | |
| 40 mM | 0.0544 mL | 0.2721 mL | 0.5441 mL | 1.3603 mL | |
| 50 mM | 0.0435 mL | 0.2177 mL | 0.4353 mL | 1.0883 mL |
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