2. Apoptosis Autophagy Anti-infection
  3. Apoptosis Autophagy Bacterial Parasite
  4. Sanguinarine chloride

Sanguinarine chloride  (Synonyms: Sanguinarin chloride; Sanguinarium chloride; Pseudochelerythrine chloride)

Cat. No.: HY-N0052A Purity: 98.71%
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Sanguinarine (Sanguinarin) chloride, a benzophenanthridine alkaloid derived from the root of Sanguinaria Canadensis, can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.

For research use only. We do not sell to patients.

CAS No. : 5578-73-4

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Based on 15 publication(s) in Google Scholar

Other Forms of Sanguinarine chloride:

Sanguinarine chloride Related Antibodies

Top Publications Citing Use of Products

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    MKN-45 and MFC cells were treated with different concentration of Sanguinarin chloride (0, 0.01, 0.1, 10, 100 μM) for 24h, and cell viability was measured by CCK-8 assays.

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    Cell viability was measured in MKN-45 and MFC cells treated with Sanguinarin chloride (2 μM) with Fer-1 (10 μM), Z-VAD (10 μM), or Nec-1 (10 μM) for 24h.

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    MKN-45 and MFC cells were treated with Sanguinarin chloride (2 μM) for 24 h. MDA level was detected by MDA assay kit.

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    Prediction of Sanguinarin chloride-targeted genes, GC-related proteins, and ferroptosis driver genes, respectively. Two overlapping proteins are displayed.

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    GETSA was performed after 24h of Sanguinarin chloride (2 μM) action, and the protein stability of NOS2 was examined by western at 40-80℃.

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    Cell lysates were incubated with Sanguinarin chloride (2 μM, 5 μM, 10 μM), digested with PronaseE, the reaction was terminated by the addition of allozyme inhibitor, and the protein level of NOS2 was detected by western.

    Sanguinarine chloride purchased from MedChemExpress. Usage Cited in: Am J Chin Med. 2025;53(5):1545-1571.  [Abstract]

    ROS level in MKN-45 and MFC cells transfected with NOS2 or siNOS2 incubated with Sanguinarin chloride was detected by DCFH-DA.

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    • Biological Activity

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    • Purity & Documentation

    • References

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    Description

    Sanguinarine (Sanguinarin) chloride, a benzophenanthridine alkaloid derived from the root of Sanguinaria Canadensis, can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.

    IC50 & Target

    Apoptosis[1]
    Cellular Effect
    Cell Line Type Value Description References
    A549 IC50
    1.59 μM
    Compound: 1; SA
    Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 33479636]
    HeLa IC50
    0.16 μg/mL
    Compound: 6
    Growth inhibition of human HeLa S3 cells after 72 hrs
    Growth inhibition of human HeLa S3 cells after 72 hrs
    		[PMID: 10395504]
    HeLa IC50
    2.43 μM
    Compound: 1; SA
    Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Antiproliferative activity against human HeLa cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 33479636]
    NCI-H1975 IC50
    1.32 μM
    Compound: 1; SA
    Antiproliferative activity against human H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    Antiproliferative activity against human H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
    		[PMID: 33479636]
    In Vitro

    Sanguinarine (SANG)-induced apoptosis is associated with the activation of JNK and NF-κB signal pathways.To determine the effects of Sanguinarine on cell viability, 22B-cFluc cells are stimulated with different concentrations of Sanguinarine for 24 h, and then a CKK-8 assay is performed. The treatment with Sanguinarine decreases the proliferation of 22B cells in a dose-dependent manner. Meanwhile, the cytosolic extracts of 22B-cFluc cells treated with different dose of Sanguinarine are measured to detect cellular caspase-3 activity using Ac-DEVD-pNA, which is a validated caspase-3 substrate. The absorbance at 450 nm increases in a dose-dependent manner, indicating increased caspase-3 activity stimulated by Sanguinarine[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sanguinarine chloride Related Antibodies
    In Vivo

    To evaluate the apoptosis induced by Sanguinarine (SANG) in vivo, 22B-cFluc cells are inoculated subcutaneously into one flank of nude mice and xenograft models are allowed to establish. Mice are treated intravenously with 10 mg/kg of Sanguinarine. At 24, 48 and 72 h after treatment, bioluminescent imaging is performed after i.p. injection of mice with 150 mg/kg of D-luciferin substrate. Sanguinarine treatment induces an obvious increase of luminescent signal as early as 48 h after initial treatment. A sustained bioluminescent imaging (BLI) intensity increased is observed throughout the course of experiment. At 72 h after treatment, the tumors are collected and subjected to TUNEL staining for evaluating apoptosis. Compared with the control tumors, the group treated with Sanguinarine exhibits significantly more cell apoptosis, indicated by the increased green signals from the sporadic apoptotic cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    367.78

    Formula

    C20H14ClNO4
    CAS No.
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    C[N+]1=CC2=C3C(OCO3)=CC=C2C(C=CC4=C5)=C1C4=CC6=C5OCO6.[Cl-]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 3.33 mg/mL (9.05 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7190 mL 13.5951 mL 27.1902 mL
    5 mM 0.5438 mL 2.7190 mL 5.4380 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.33 mg/mL (0.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.33 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (3.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.33 mg/mL (0.90 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.33 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (3.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 25 mg/mL (67.98 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.93%

    SDS (394 KB)

    COA (253 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    The caspase-3 activity is measured using a caspase-3 activity assay kit. Briefly, the cells treated by different concentrations of Sanguinarine (0.5 μM, 1 μM, 2 μM, 4 μM) or control DMSO are collected, washed and lysed in a lysis buffer for 30 min on ice. The supernatants are then collected by centrifuging at 1,2000 rpm for 10 min. The Ac-DEVD-pNA (2 mM) is added to each sample and incubated at 37°C for 1 h. The optical density (OD) of each sample is finally quantified at a wavelength of 405 nm using a spectrophotometer. The p-NA standard is used to calibrate the caspase-3 activity of each sample[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [1]

    The cell viability of Sanguinarine is determined by CCK-8 assay using a cell counting kit-8. Briefly, 22B-cFluc cells are seeded in a 96-well plate (5×103 cells/well) and treated with different concentrations of Sanguinarine (0.5 μM, 1 μM, 2 μM, 4 μM) for 24 h. Then 10 mL CKK-8 is added to each well for 4 h and the absorbance at 450 nm is measured with a microplate reader. The optical density (OD) values are determined to reflect the viable cell populations from each well[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice[1]
    Xenografted tumor models are prepared by injection of 1×107 22B-cFluc cells suspended in PBS into nude mouse (n=6). After tumors reach a volume of approximately 100 mm3, Sanguinarine (10 mg/kg) is i.v. injected into mice. After injection for 24 h, 48 h and 72 h, mice are given a single i.p. dose of 150 mg/kg D-luciferin and bioluminescence imaging are performed using a Xenogen Lumina II system. The signal intensity in the region of interest is expressed using the Living Image software 4.1. For the anti-tumor therapy studies, one group of tumor-bearing mice (n=6) receive intravenously 10 mg/kg of Sanguinarine every other day throughout the experimental period, while the control group of mice (n=6) receive DMSO only. Tumor growth measurement is calculated[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.7190 mL 13.5951 mL 27.1902 mL 67.9754 mL
    5 mM 0.5438 mL 2.7190 mL 5.4380 mL 13.5951 mL
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    Sanguinarine chloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sanguinarine5578-73-4SanguinarinSanguinariumPseudochelerythrineApoptosisAutophagyBacterialParasiteInhibitorinhibitorinhibit

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