2. Protein Tyrosine Kinase/RTK Metabolic Enzyme/Protease
  3. SHP1 Phosphatase
  4. TPI-1

TPI-1, also known as Tyrosine Phosphatase Inhibitor 1, is a SHP-1 inhibitor; inhibits recombinant SHP-1 with an IC50 of 40 nM.

For research use only. We do not sell to patients.

CAS No. : 79756-69-7

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Customer Review

Based on 20 publication(s) in Google Scholar

TPI-1 Related Antibodies

Top Publications Citing Use of Products

    TPI-1 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 Apr 17;13(4):e010879.  [Abstract]

    Mice bearing YUMM 1.1 tumors were treated intraperitoneally (i.p.) with TPI-1 (3 mg/kg) in combination with ICI (anti-PD-1 and anti-CTLA), or singly with either TPI-1 or ICI, every 3 days beginning at the time of tumor visibility (day 7–10).

    TPI-1 purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2024 Oct 29;20(10):e1012674.  [Abstract]

    Wild-type and VCP-deficient BMDM cells were pretreated with DMSO or the SHP1 inhibitor TPI-1 (10 μM) for 1 hour, then stimulated with ZymD at specified time points, followed by Western blot analysis.

    TPI-1 purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 Jun 10:13:865503.  [Abstract]

    TPI-1 (1 μg/mL). Supernatant was collected 18 hours post-infection, and cytokines were detected using a sandwich ELISA method.

    TPI-1 purchased from MedChemExpress. Usage Cited in: J Allergy Clin Immunol. 2022 Jan;149(1):223-236.e6.  [Abstract]

    TPI-1 (15 μM). Representative histograms of SHP-1 expression in WT and LAIR-1 KO ILC2s and their corresponding MFI quantification.

    TPI-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2020 Mar 9;17(1):81.  [Abstract]

    After overexpressing DJ-1 and inhibiting SHP-1 with virus and TPI-1 (10 ng/ml), respectively, the expression of NLRX1, TRAF6 and SHP-1 in rat brain tissue was detected by Western blotting.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    TPI-1, also known as Tyrosine Phosphatase Inhibitor 1, is a SHP-1 inhibitor; inhibits recombinant SHP-1 with an IC50 of 40 nM.

    IC50 & Target

    IC50: 40 nM (recombinant SHP-1)[1]
    In Vitro

    SHP-1 has been implicated as a potential cancer therapeutic target. TPI-1 is effective starting at 10 ng/mL in increasing SHP-1 phospho-substrates pLck-pY394. TPI-1 selectively increases SHP-1 phospho-substrates (pLck-pY394, pZap70 and pSlp76) in Jurkat T cells but has little effects on pERK1/2 or pLck-pY505. TPI-1 induces mouse splenic-IFNγ+ cells and induces IFNγ+ cells in human peripheral blood[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TPI-1 Related Antibodies
    In Vivo

    TPI-1 inhibits the growth of B16 melanoma tumors in mice at a tolerated oral dose in a T cell-dependent manner but has little effects on B16 cell growth in culture. TPI-1 thus also increases pLck-pY394 and IFNγ+ cells in mice. TPI-1 also inhibits B16 tumor growth and prolongs tumor mice survival as a tolerated s.c. agent[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    253.08

    Formula

    C12H6Cl2O2
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    ClC1=CC(C2=CC(C=CC2=O)=O)=C(Cl)C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (197.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9513 mL 19.7566 mL 39.5132 mL
    5 mM 0.7903 mL 3.9513 mL 7.9026 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (9.88 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (250 KB) HNMR (236 KB)

    Handling Instructions (2659 KB)
    References
    Animal Administration
    [1]

    Mice: For in vivo induction of pLck-pY394 and IFNγ+ cells in mice, C57BL/6J mice are treated with PBS or TPI-1 (1 or 3 mg/kg, s.c.) for 4 days. Spleens are harvested one hour post-treatment on day 4 and processed into splenocytes, which are used for assessing pLck-pY394 levels by SDS-PAGE/Western blotting and for quantification of IFNγ+ cells by ELISPOT assays. Mice are also treated with TPI-1 (10 mg/kg, daily, s.c.) to evaluate the toxicity of the compounds in vivo[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.9513 mL 19.7566 mL 39.5132 mL 98.7830 mL
    5 mM 0.7903 mL 3.9513 mL 7.9026 mL 19.7566 mL
    10 mM 0.3951 mL 1.9757 mL 3.9513 mL 9.8783 mL
    15 mM 0.2634 mL 1.3171 mL 2.6342 mL 6.5855 mL
    20 mM 0.1976 mL 0.9878 mL 1.9757 mL 4.9391 mL
    25 mM 0.1581 mL 0.7903 mL 1.5805 mL 3.9513 mL
    30 mM 0.1317 mL 0.6586 mL 1.3171 mL 3.2928 mL
    40 mM 0.0988 mL 0.4939 mL 0.9878 mL 2.4696 mL
    50 mM 0.0790 mL 0.3951 mL 0.7903 mL 1.9757 mL
    60 mM 0.0659 mL 0.3293 mL 0.6586 mL 1.6464 mL
    80 mM 0.0494 mL 0.2470 mL 0.4939 mL 1.2348 mL
    100 mM 0.0395 mL 0.1976 mL 0.3951 mL 0.9878 mL
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    TPI-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TPI-179756-69-7TPI1TPI 1SHP1PhosphataseShp1PTPN6Src homology region 2 (SH-2) domain-containing phosphatase 1Inhibitorinhibitorinhibit

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