2. Metabolic Enzyme/Protease
    Stem Cell/Wnt
    JAK/STAT Signaling
    Apoptosis
  3. Phosphatase
    STAT
    Apoptosis
  4. SC-43

SC-43 

Cat. No.: HY-136657 Purity: 98.61%
COA Handling Instructions

SC-43, a Sorafenib derivative, is a potent and orally active SHP-1 (PTPN6) agonist. SC-43 inhibits the phosphorylation of STAT3 and induces cell apoptosis. SC-43 has anti-fibrotic and anticancer effects.

For research use only. We do not sell to patients.

SC-43 Chemical Structure

SC-43 Chemical Structure

CAS No. : 1400989-25-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 275 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 275 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 250 In-stock
Estimated Time of Arrival: December 31
10 mg USD 350 In-stock
Estimated Time of Arrival: December 31
25 mg USD 650 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE SC-43

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SC-43, a Sorafenib derivative, is a potent and orally active SHP-1 (PTPN6) agonist. SC-43 inhibits the phosphorylation of STAT3 and induces cell apoptosis. SC-43 has anti-fibrotic and anticancer effects[1][2].

IC50 & Target[1][2]

SHP-1

 

p-STAT3

 

In Vitro

SC-43 (0-10 μM; 24-72 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment reveals the anti-proliferative effects in cholangiocarcinoma (CCA) cell lines in a dose-dependent manner after treating 24, 48 and 72 hours respectively[1].
SC-43 (0-10 μM; 24 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment shows increased sub-G1 cells and G2-M arrest, indicating SC-43 induced differential apoptotic effects in these cell lines[1].
SC-43 (0-10 μM; 24 hours; HuCCT-1, KKU-100, and CGCCA cells) treatment demonstrates significant increase in cleaved caspase-3 and PARP level[1].
SC-43 activates SH2 domain-containing phosphatase 1 (SHP-1) activity, leading to p-STAT3 and downstream cyclin B1 and Cdc2 downregulation. SC-43 augments SHP-1 activity by direct binding to N-SH2 and relief of its autoinhibition[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: HuCCT-1, KKU-100, and CGCCA cells
Concentration: 0 μM, 0.25 μM, 0.5 μM, 0.75 μM, 1 μM, 2.5 μM, 5 μM, 10 μM
Incubation Time: 24 hours, 48 hours, 72 hours
Result: Revealed the anti-proliferative effects in CCA cell lines in a dose-dependent manner after treating 24, 48 and 72 hours respectively.

Cell Cycle Analysis[1]

Cell Line: HuCCT-1, KKU-100, and CGCCA cells
Concentration: 0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM
Incubation Time: 24 hours
Result: Showed increased sub-G1 cells and G2-M arrest.

Western Blot Analysis[1]

Cell Line: HuCCT-1, KKU-100, and CGCCA cells
Concentration: 0 μM, 1 μM, 2.5 μM, 5 μM, 10 μM
Incubation Time: 24 hours
Result: Demonstrated significant increase in cleaved caspase-3 and PARP level.
In Vivo

SC-43 (10-30 mg/kg; oral gavage; daily; for 23 days; male NCr athymic nude mice) treatment exhibits xenograft tumor growth inhibition, p-STAT3 reduction and SHP-1 activity elevation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NCr athymic nude mice (5-7 weeks of age) injected with HuCCT-1 cells[1]
Dosage: 10 mg/kg or 30 mg/kg
Administration: Oral gavage; daily; for 23 days
Result: Exhibited xenograft tumor growth inhibition, p-STAT3 reduction and SHP-1 activity elevation.
Clinical Trial
Molecular Weight

431.80

Appearance

Solid

Formula

C21H13ClF3N3O2
CAS No.
SMILES

O=C(NC1=CC=CC(OC2=CC=C(C#N)C=C2)=C1)NC3=CC=C(Cl)C(C(F)(F)F)=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (578.97 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3159 mL 11.5794 mL 23.1589 mL
5 mM 0.4632 mL 2.3159 mL 4.6318 mL
10 mM 0.2316 mL 1.1579 mL 2.3159 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.08 mg/mL (4.82 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.82 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

SC-431400989-25-4SC43SC 43PhosphataseSTATApoptosisSHP-1PTPN6antiproliferativep-STAT3anti-fibroticanticancerorallycaspase-3PARPInhibitorinhibitorinhibit

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