Substance P TFA
Based on 32 publication(s) in Google Scholar
Substance P TFA (Neurokinin P TFA) is a CNS-penetrant neuropeptide, acting as a neurotransmitter and as a neuromodulator in the CNS. The endogenous receptor for substance P is neurokinin 1 receptor (NK1-receptor, NK1R).
For research use only. We do not sell to patients.
- Purity: 99.34%
- CAS No.: 148470-19-3
- Formula: C65H99F3N18O15S
- Molecular Weight:1461.65
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Storage:
Stored under nitrogen, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Substance P TFA
More- Bioact Mater. 2026 Feb 16:61:258-271. [Abstract]
- Cell Discov. 2023 Jun 30;9(1):66. [Abstract]
- Int J Biol Sci. 2024 Apr 22;20(7):2507-2531. [Abstract]
- Free Radic Biol Med. 2023 Oct 5;209(Pt 1):1-8. [Abstract]
- J Headache Pain. 2024 Aug 28;25(1):141. [Abstract]
- J Transl Med. 2025 Dec 1;23(1):1367. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2026;20(5):101741. [Abstract]
- Prog Neurobiol. 2021 Jul:202:102041. [Abstract]
- J Invest Dermatol. 2025 Nov 4:S0022-202X(25)03517-1. [Abstract]
- J Invest Dermatol. 2023 May;143(5):812-821.e3. [Abstract]
- Cell Prolif. 2019 Jan;52(1):e12527. [Abstract]
- Cells. 2026 Feb 3;15(3):287. [Abstract]
- Eur J Pharmacol. 2025 Sep 24:178189. [Abstract]
- Eur J Pharmacol. 2023 Sep 15:955:175902. [Abstract]
- FASEB J. 2025 Jul 31;39(14):e70817. [Abstract]
- iScience. 2023 Apr 18;26(5):106688. [Abstract]
- Sci Rep. 2026 Feb 9;16(1):7851. [Abstract]
- Food Sci Nutr. 2025 Sep 1;13(9):e70877. [Abstract]
- J Immunol Res. 2022 May 12;2022:5582811. [Abstract]
- Mol Med Rep. 2022 Feb;25(2):50. [Abstract]
- Immunol Lett. 2024 Dec:270:106934. [Abstract]
- Mol Biol Rep. 2022 Mar;49(3):2227-2236. [Abstract]
- J Am Soc Mass Spectrom. 2020 Jul 5. [Abstract]
- Reprod Sci. 2026 Mar 19. [Abstract]
- Chin J Integr Med. 2025 Dec;31(12):1097-1104. [Abstract]
- SSRN. 2026 Jun 16.
- SSRN. 2025 Feb 24.
- Authorea. 2023 Aug 14.
- Research Square Preprint. 2021 Dec.
- Research Square Preprint. 2021 Sep.
- Research Square Preprint. 2021 Jul.
- Chin J Comp Med. May 2018,Vol. 28. No. 5
Customer Validation & Images
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Histological Imaging/Staining
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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RT-PCR
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Cell Imaging/Staining
All Endogenous Metabolite Isoforms
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Biological Activity
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NK1R |
Human Endogenous Metabolite |
The neuropeptide substance P (SP) that are mediated by the neurokinin 1 receptor (NK1-R) desensitize and resensitize, which may be associated with NK1-R endocytosis and recycling. SP and the NK1-R are internalized into the same clathrin immunoreactive vesicles, and then sorted into different compartments. SP is intact at the cell surface and in early endosomes, but slowly degraded in perinuclear vesicles. SP induces clathrin-dependent internalization of the NK1-R. The SP/NK1-R complex dissociates in acidified endosomes. SP is degraded, whereas the NK1-R recycles to the cell surface. SP induces internalization of the NK1-R both in transfected epithelial cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 148470-19-3
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Appearance Solid
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Molecular Weight 1461.65
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Formula C65H99F3N18O15S
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Color White to off-white
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Synonyms
Neurokinin P TFA
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Sequence
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2
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Sequence Shortening
RPKPQQFFGLM-NH2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Stored under nitrogen, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
Publications (32)
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Journal Impact Factor
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Most Recent
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Bioact Mater
Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy. [Abstract]2026 Feb 16:61:258-271. PMID: 41737639 -
Cell Discov
Structural insights into neurokinin 3 receptor activation by endogenous and analogue peptide agonists. [Abstract]2023 Jun 30;9(1):66. PMID: 37391393 -
Int J Biol Sci
Neuropeptide substance P attenuates colitis by suppressing inflammation and ferroptosis via the cGAS-STING signaling pathway. [Abstract]2024 Apr 22;20(7):2507-2531. PMID: 38725846
Substance P TFA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 22;20(7):2507-2531. [Abstract]
Substance P (SP, 5 nmol/kg; i.v.; once daily for 6 days) alleviates pathological indexes in DSS-induced colitis mice. Representative images of H&E sections and histological score. Scale bar = 100 μm.
Substance P TFA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 22;20(7):2507-2531. [Abstract]
Substance P (SP, 5 nmol/kg; i.v.; once daily for 6 days) alleviates pathological indexes in DSS-induced colitis mice. Photographs of colon length.
Substance P TFA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 22;20(7):2507-2531. [Abstract]
Substance P (SP, 5 nmol/kg; i.v.; once daily for 6 days) alleviates pathological indexes in DSS-induced colitis mice. (Images spleen)
Substance P TFA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 22;20(7):2507-2531. [Abstract]
RT-qPCR assay of inflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-8) in TNF-α-stimulated Caco-2 cells treated with/without Substance P (SP, 25, 50, and 100 nmol) for 24 h. Data are obtained from three independent experiments, and expressed as the mean ± SEM; n = 5-8.
Substance P TFA purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 22;20(7):2507-2531. [Abstract]
Representative picture of JC-1 staining in TNF-α-treated Caco-2 cells incubated with Substance P (SP, 100 nmol) for 24 h.
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Free Radic Biol Med
H2O2 enhances the spontaneous phasic contractions of isolated human-bladder strips via activation of TRPA1 channels on sensory nerves and the release of substance P and PGE2. [Abstract]2023 Oct 5;209(Pt 1):1-8. PMID: 37802373 -
J Headache Pain
Comparison of gepant effects at therapeutic plasma concentrations: connecting pharmacodynamics and pharmacokinetics. [Abstract]2024 Aug 28;25(1):141. PMID: 39198753 -
J Transl Med
The role of substance P in Th17/Treg imbalance and ocular surface damage in chronic allergic conjunctivitis. [Abstract]2025 Dec 1;23(1):1367. PMID: 41327447 -
Cell Mol Gastroenterol Hepatol
Mast Cells Inhibit Stem Cell-driven Epithelial Repair in Inflammatory Bowel Disease via Suppressing Wnt/lrp6/β-Catenin Signaling Pathway. [Abstract]2026;20(5):101741. PMID: 41616821 -
Prog Neurobiol
2021 Jul:202:102041. PMID: 33766679 -
J Invest Dermatol
MRGPRX2-Mediated Mast Cell Activation Promotes Malignant Progression of Cutaneous Squamous Cell Carcinoma through IL-17A Release. [Abstract]2025 Nov 4:S0022-202X(25)03517-1. PMID: 41197765 -
J Invest Dermatol
The key role of CB1R in the sensory neurons to regulate psoriasiform skin inflammation and pruritus. [Abstract]2023 May;143(5):812-821.e3. PMID: 36410425 -
Cell Prolif
MiR-34b/c-5p and the neurokinin-1 receptor regulate breast cancer cell proliferation and apoptosis. [Abstract]2019 Jan;52(1):e12527. PMID: 30334298 -
Cells
Identification of Natural Compounds Triggering MRGPRX2-Mediated Calcium Flux and Degranulation in RBL-2H3 Cells. [Abstract]2026 Feb 3;15(3):287. PMID: 41677650 -
Eur J Pharmacol
Salicylic acid inhibits Mas-related G protein-coupled receptor X2-mediated mast cell 2 activation and mitigates cutaneous pseudo-allergic reactions. [Abstract]2025 Sep 24:178189. PMID: 41005604 -
Eur J Pharmacol
Effects of 101BHG-D01, a novel M receptor antagonism, on allergic rhinitis in animal models and its mechanism. [Abstract]2023 Sep 15:955:175902. PMID: 37422119 -
FASEB J
Nitric Oxide Scavenging Alleviates Scar Pruritus by Inhibiting S-Nitrosylation of Transient Receptor Potential Channels and Limiting Calcium Influx. [Abstract]2025 Jul 31;39(14):e70817. PMID: 40637249 -
iScience
Trigeminal nerve-derived substance P regulates limbal stem cells by the PI3K-AKT pathway. [Abstract]2023 Apr 18;26(5):106688. PMID: 37187698 -
Sci Rep
2026 Feb 9;16(1):7851. PMID: 41663512 -
Food Sci Nutr
Evaluation of the Effects of Chitin and Chitosan on Pseudo-Allergic Reaction by Inhibiting MRGPRX2 Activation. [Abstract]2025 Sep 1;13(9):e70877. PMID: 40901656 -
J Immunol Res
A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641. [Abstract]2022 May 12;2022:5582811. PMID: 35600049 -
Mol Med Rep
Substance P prevents doxorubicin‑induced cardiomyocyte injury by regulating apoptosis and autophagy: In vitro and in vivo evidence. [Abstract]2022 Feb;25(2):50. PMID: 34913064 -
Immunol Lett
Ursolic acid attenuates pseudo-allergic reactions via reducing MRGPRX2-mediated mast cell degranulation. [Abstract]2024 Dec:270:106934. PMID: 39395727 -
Mol Biol Rep
Substance P modulates BMSCs migration for tissue repair through NK-1R/CXCR4/p-Akt signal activation. [Abstract]2022 Mar;49(3):2227-2236. PMID: 35034285 -
J Am Soc Mass Spectrom
Wire Desorption Combined with Electrospray Ionization Mass Spectrometry: Direct Analysis of Small Organic and Large Biological Compounds. [Abstract]2020 Jul 5. PMID: 32559077 -
Reprod Sci
Exposure to Green Light-emitting Diodes Alleviates Endometriosis-associated Pain in Mice with Induced Deep Endometriosis. [Abstract]2026 Mar 19. PMID: 41854839 -
Chin J Integr Med
Inhibitory Effects of Nardostachys Jatamansi DC. Volatile Oil on Psychological Factors SP/CORT-Induced Hyperpigmentation. [Abstract]2025 Dec;31(12):1097-1104. PMID: 41342982 -
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Solvent & Solubility
H2O : 100 mg/mL (68.42 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (34.21 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Grady EF, et al. Delineation of the endocytic pathway of substance P and its seven-transmembrane domain NK1 receptor. Mol Biol Cell. 1995 May;6(5):509-24. [Content Brief]
[2]. Zhang L, et al. MiR-34b/c-5p and the neurokinin-1 receptor regulate breast cancer cell proliferation and apoptosis. Cell Prolif. 2018 Oct 17:e12527. [Content Brief]
[3]. Chappa AK, et al. Characteristics of substance P transport across the blood-brain barrier. Pharm Res. 2006 Jun;23(6):1201-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O | 1 mM | 0.6842 mL | 3.4208 mL | 6.8416 mL | 17.1040 mL |
| 5 mM | 0.1368 mL | 0.6842 mL | 1.3683 mL | 3.4208 mL | |
| 10 mM | 0.0684 mL | 0.3421 mL | 0.6842 mL | 1.7104 mL | |
| 15 mM | 0.0456 mL | 0.2281 mL | 0.4561 mL | 1.1403 mL | |
| 20 mM | 0.0342 mL | 0.1710 mL | 0.3421 mL | 0.8552 mL | |
| 25 mM | 0.0274 mL | 0.1368 mL | 0.2737 mL | 0.6842 mL | |
| 30 mM | 0.0228 mL | 0.1140 mL | 0.2281 mL | 0.5701 mL | |
| 40 mM | 0.0171 mL | 0.0855 mL | 0.1710 mL | 0.4276 mL | |
| 50 mM | 0.0137 mL | 0.0684 mL | 0.1368 mL | 0.3421 mL | |
| 60 mM | 0.0114 mL | 0.0570 mL | 0.1140 mL | 0.2851 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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