1. GPCR/G Protein Apoptosis
  2. LPL Receptor Apoptosis
  3. JTE-013

JTE-013 is a potent and specific S1P2 (Sphingosine-1-Phosphate 2; EDG-5) antagonist. JTE-013 inhibits the specific binding of radiolabeled S1P to human and rat S1P2 with IC50s of 17 nM and 22 nM, respectively.

For research use only. We do not sell to patients.

JTE-013 Chemical Structure

JTE-013 Chemical Structure

CAS No. : 383150-41-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 97 In-stock
Solution
10 mM * 1 mL in DMSO USD 97 In-stock
Solid
5 mg USD 88 In-stock
10 mg USD 132 In-stock
50 mg USD 495 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products

    JTE-013 purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2023 Jan 11;14(1):4.  [Abstract]

    JTE-013 significantly inhibits the proliferation ability of HCC cells.

    JTE-013 purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2023 Jan 11;14(1):4.  [Abstract]

    JTE-013 (0, 24, and 48 h) significantly inhibits the growth rate of Huh7 and HepG2 cells.

    JTE-013 purchased from MedChemExpress. Usage Cited in: Discov Oncol. 2023 Jan 11;14(1):4.  [Abstract]

    JTE-013 significantly inhibits the proliferation and invasive ability of HCC cells.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    JTE-013 is a potent and specific S1P2 (Sphingosine-1-Phosphate 2; EDG-5) antagonist. JTE-013 inhibits the specific binding of radiolabeled S1P to human and rat S1P2 with IC50s of 17 nM and 22 nM, respectively[1].

    IC50 & Target

    IC50: 17 nM (S1P2 for human) and 22 nM (S1P2 for rat)[1]

    In Vitro

    JTE-013 (50-200 μM; 1-3 days) reduces cell viability[1].
    ? JTE-013 (10-1000 nM; 30 mins) reverses S1P-induced Akt inhibition and inhibits S1P-induced ERK activation[1].
    ? JTE-013 displays 4.2% inhibition of S1P3 and does not antagonize S1P1 at concentrations up to 10 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: SK-N-AS cells
    Concentration: 50, 100, 150, 200 μM
    Incubation Time: 1-3 days
    Result: Reduced cell viability.

    Western Blot Analysis[1]

    Cell Line: SK-N-AS cells
    Concentration: 10, 100, 1000 nM
    Incubation Time: 30 mins
    Result: Reversed S1P-induced Akt inhibition and inhibited S1P-induced ERK activation.
    In Vivo

    JTE-013 (gavage; 30 mg/kg daily for 14 consecutive days) reduces tumor size and tumor weight[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Six-week-old female athymic NCr-nu/nu nude mice[1]
    Dosage: 30 mg/kg
    Administration: Gavage; daily for 14 consecutive days
    Result: Reduced tumor size and tumor weight.
    Molecular Weight

    408.29

    Formula

    C17H19Cl2N7O

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(C1=C2C(N(C)N=C2C)=NC(NNC(NC3=CC(Cl)=NC(Cl)=C3)=O)=C1)C

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (244.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4492 mL 12.2462 mL 24.4924 mL
    5 mM 0.4898 mL 2.4492 mL 4.8985 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months. When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.83 mg/mL (2.03 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 0.83 mg/mL (2.03 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.57%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 years; -20°C, 6 months. When stored at -80°C, please use it within 1 years. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.4492 mL 12.2462 mL 24.4924 mL 61.2310 mL
    5 mM 0.4898 mL 2.4492 mL 4.8985 mL 12.2462 mL
    10 mM 0.2449 mL 1.2246 mL 2.4492 mL 6.1231 mL
    15 mM 0.1633 mL 0.8164 mL 1.6328 mL 4.0821 mL
    20 mM 0.1225 mL 0.6123 mL 1.2246 mL 3.0615 mL
    25 mM 0.0980 mL 0.4898 mL 0.9797 mL 2.4492 mL
    30 mM 0.0816 mL 0.4082 mL 0.8164 mL 2.0410 mL
    40 mM 0.0612 mL 0.3062 mL 0.6123 mL 1.5308 mL
    50 mM 0.0490 mL 0.2449 mL 0.4898 mL 1.2246 mL
    60 mM 0.0408 mL 0.2041 mL 0.4082 mL 1.0205 mL
    80 mM 0.0306 mL 0.1531 mL 0.3062 mL 0.7654 mL
    100 mM 0.0245 mL 0.1225 mL 0.2449 mL 0.6123 mL
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    JTE-013 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    JTE-013
    Cat. No.:
    HY-100675
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