1. GPCR/G Protein
    Apoptosis
  2. LPL Receptor
    Apoptosis
  3. JTE-013

JTE-013 

Cat. No.: HY-100675 Purity: 99.57%
Handling Instructions

JTE-013 is a potent and specific S1P2 (Sphingosine-1-Phosphate 2; EDG-5) antagonist. JTE-013 inhibits the specific binding of radiolabeled S1P to human and rat S1P2 with IC50s of 17 nM and 22 nM, respectively.

For research use only. We do not sell to patients.

JTE-013 Chemical Structure

JTE-013 Chemical Structure

CAS No. : 383150-41-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 105 In-stock
Estimated Time of Arrival: December 31
25 mg USD 225 In-stock
Estimated Time of Arrival: December 31
50 mg USD 390 In-stock
Estimated Time of Arrival: December 31
100 mg USD 670 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

JTE-013 is a potent and specific S1P2 (Sphingosine-1-Phosphate 2; EDG-5) antagonist. JTE-013 inhibits the specific binding of radiolabeled S1P to human and rat S1P2 with IC50s of 17 nM and 22 nM, respectively[1].

IC50 & Target

IC50: 17 nM (S1P2 for human) and 22 nM (S1P2 for rat)[1]

In Vitro

JTE-013 (50-200 μM; 1-3 days) reduced cell viability[1].
JTE-013 (10-1000 nM; 30 mins) reverses S1P-induced Akt inhibition and inhibits S1P-induced ERK activation[1].
JTE-013 displays 4.2% inhibition of S1P3 and does not antagonize S1P1 at concentrations up to 10 μM[1].

Cell Viability Assay[1]

Cell Line: SK-N-AS cells
Concentration: 50, 100, 150, 200 μM
Incubation Time: 1-3 days
Result: Reduced cell viability.

Western Blot Analysis[1]

Cell Line: SK-N-AS cells
Concentration: 10, 100, 1000 nM
Incubation Time: 30 mins
Result: Reversed S1P-induced Akt inhibition and inhibited S1P-induced ERK activation.
In Vivo

JTE-013 (gavage; 30 mg/kg daily for 14 consecutive days) reduces tumor size and tumor weight[1].

Animal Model: Six-week-old female athymic NCr-nu/nu nude mice[1]
Dosage: 30 mg/kg
Administration: Gavage; daily for 14 consecutive days
Result: Reduced tumor size and tumor weight.
Molecular Weight

408.29

Formula

C₁₇H₁₉Cl₂N₇O

CAS No.

383150-41-2

SMILES

CC(C1=C2C(N(C)N=C2C)=NC(NNC(NC3=CC(Cl)=NC(Cl)=C3)=O)=C1)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (244.92 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4492 mL 12.2462 mL 24.4924 mL
5 mM 0.4898 mL 2.4492 mL 4.8985 mL
10 mM 0.2449 mL 1.2246 mL 2.4492 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 0.83 mg/mL (2.03 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.83 mg/mL (2.03 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

JTE-013JTE013JTE 013LPL ReceptorApoptosisLysophospholipid ReceptorInhibitorinhibitorinhibit

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Product name:
JTE-013
Cat. No.:
HY-100675
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