2. Cell Cycle/DNA Damage
  3. Eukaryotic Initiation Factor (eIF)
  4. Kurarinone

Kurarinone is an orally active flavonoid isolated from matrine that inhibits the pathogenesis of experimental autoimmune encephalomyelitis by inhibiting cell differentiation of Th1 and Th17. Kurarinone has antitumor and anti-inflammatory activity.

For research use only. We do not sell to patients.

CAS No. : 34981-26-5

Size Price Stock Quantity
Free Sample (0.1 - 0.2 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Kurarinone Related Antibodies

Top Publications Citing Use of Products

    Kurarinone purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 27.  [Abstract]

    BMMs were treated with Kurarinone (KR) (0, 2.5, 5, 10, 15, 20, 30, 60 μM) at varying concentrations for 48 h. Subsequently, the cell viability was evaluated using the CCK-8 assay.

    Kurarinone purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 27.  [Abstract]

    Fluorescence microscopy images illustrate the characteristic structures of F-actin, stained with phalloidin, highlighting the presence of F-actin rings (green) alongside nuclei (blue) treated with Kurarinone (KR) (10, 20 μM).

    Kurarinone purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 27.  [Abstract]

    Quantitative PCR (qPCR) analysis revealed that the expression levels of specific osteoclast markers were diminished following treatment with varying concentrations of Kurarinone (KR) (0, 5, 10, 20 μM), in contrast to the positive control group.

    Kurarinone purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 27.  [Abstract]

    Protein Bands of Key Osteoclast Markers treated with Kurarinone (KR) (0, 5, 10, 20 μM).

    Kurarinone purchased from MedChemExpress. Usage Cited in: Inflammation. 2025 Jan 27.  [Abstract]

    Detection of ROS levels via flow cytometry treated with Kurarinone (KR) (0, 10, 20 μM).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Kurarinone is an orally active flavonoid isolated from matrine that inhibits the pathogenesis of experimental autoimmune encephalomyelitis by inhibiting cell differentiation of Th1 and Th17. Kurarinone has antitumor and anti-inflammatory activity[1][2][3].

    In Vitro

    Kurarinone (50 μM，6 h) activates ATF4 via the PERK-eIF2α signaling pathway and exerts cytostatic effects on cancer cells[1].
    Ergothioneine (0.25，1 mM，23 h) can regulate PC12 DNA damage，MAPKs activation and cell death induced by hydrogen peroxide[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Kurarinone Related Antibodies

    Cell Viability Assay[2]

    Cell Line: PC3
    Concentration: 10, 20, 50 μM
    Incubation Time: 48 h
    Result: Suppressed the proliferation of PC3 cells in a dose-dependent manner.

    Western Blot Analysis[2]

    Cell Line: PC3, HeLa, TIG1
    Concentration: 50 μM
    Incubation Time: 6 h
    Result: Upregulated the expression of TRB3 and ATF4 proteins.
    Induced PERK phosphorylation and p21 protein expression.
    Reduced the expression of cyclin D1 and cyclin A.
    In Vivo

    Kurarinone (100 mg/kg，口服，第 21-42 天) 在胶原诱导关节炎 (CIA) 小鼠模型中具有治疗作用[3]。
    ? Kurarinone (100 mg/kg，p.o.，from day 21-42) has a therapeutic effect in a mouse model of collagen-induced arthritis (CIA)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: CIA Mouse Model[3]
    Dosage: 100 mg/kg
    Administration: p.o.
    Result: Reduced the levels of proinflammatory cytokines, TNF-α, IL-6, IFN-γ, and IL-17A.
    Reduced STAT1 and STAT3 phosphorylation and the proportions of Th1 and Th17 cells in lymph nodes.
    Molecular Weight

    438.51

    Formula

    C26H30O6
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C1C[C@@H](C2=CC=C(O)C=C2O)OC3=C(C[C@H](C(C)=C)C/C=C(C)\C)C(O)=CC(OC)=C13
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (114.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2804 mL 11.4022 mL 22.8045 mL
    5 mM 0.4561 mL 2.2804 mL 4.5609 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  20% HP-β-CD in Saline

      Solubility: 10 mg/mL (22.80 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.47%

    SDS (393 KB)

    COA (250 KB) HNMR (235 KB) RP-HPLC (141 KB) MS (849 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2804 mL 11.4022 mL 22.8045 mL 57.0112 mL
    5 mM 0.4561 mL 2.2804 mL 4.5609 mL 11.4022 mL
    10 mM 0.2280 mL 1.1402 mL 2.2804 mL 5.7011 mL
    15 mM 0.1520 mL 0.7601 mL 1.5203 mL 3.8007 mL
    20 mM 0.1140 mL 0.5701 mL 1.1402 mL 2.8506 mL
    25 mM 0.0912 mL 0.4561 mL 0.9122 mL 2.2804 mL
    30 mM 0.0760 mL 0.3801 mL 0.7601 mL 1.9004 mL
    40 mM 0.0570 mL 0.2851 mL 0.5701 mL 1.4253 mL
    50 mM 0.0456 mL 0.2280 mL 0.4561 mL 1.1402 mL
    60 mM 0.0380 mL 0.1900 mL 0.3801 mL 0.9502 mL
    80 mM 0.0285 mL 0.1425 mL 0.2851 mL 0.7126 mL
    100 mM 0.0228 mL 0.1140 mL 0.2280 mL 0.5701 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Kurarinone Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Kurarinone34981-26-5Eukaryotic Initiation Factor (eIF)FlavonoidPC3HeLaTIG1antitumoranti-inflammatoryInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Kurarinone
    Cat. No.:
    HY-N2279
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.